Standard name
Description Ribosomal 60S subunit protein L10; homologous to mammalian ribosomal protein L10 and bacterial L16; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; protein abundance increases under DNA replication stress; mutations in human homolog implicated in T-cell acute lymphoblastic leukemia and also autism spectrum disorders (ASD); human RPL10 can complement yeast null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.07 0.06 – 0 0.08 0.07 0.11 0.05 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.99 0.99 0.92 0.87 0.78 – 0.78 0.68 0.77 0.61 0.96 0.99 0.99 0.98 0.99 0.99 0.96 0.95 0.94 0.93 0.93 0.93
Endoplasmic Reticulum 0 0 0 0 0.06 0.07 0.18 – 0.16 0.21 0.14 0.26 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 8 3 – 6 11 10 13 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 4 0 – 0 2 1 2 0 0 0 1 0 0 0 1 2 1 2 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 5 2 11 20 22 – 14 33 24 35 10 4 4 0 5 1 0 0 1 0 1 0
Cytoplasm 219 413 369 250 291 258 292 – 261 268 254 199 174 408 329 330 285 304 193 397 333 184 370 404
Endoplasmic Reticulum 0 2 1 3 20 20 69 – 52 84 45 86 1 4 4 22 12 20 2 1 5 4 12 13
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 1 0 2 5 – 7 19 16 20 0 0 0 0 0 0 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 0 1 – 2 0 6 2 0 0 0 1 0 1 0 0 1 0 0 0
Nucleus 0 1 0 0 0 1 0 – 0 0 0 0 0 1 0 0 0 0 0 4 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 1 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 2 2 0 2 1 6 – 0 1 1 3 0 3 0 4 1 1 0 4 2 2 3 5
Unique Cell Count 219 417 371 253 318 298 374 333 393 330 328 182 413 332 337 288 306 201 417 355 199 396 436
Labelled Cell Count 219 419 377 256 324 314 399 342 418 357 360 185 420 337 359 303 327 201 417 355 199 396 436


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 184.5 145.5 148.8 125.5 104.5 63.9 71.7 – 62.3 64.7 59.8 62.2 159.1 152.9 140.8 185.7 201.5 186.3 151.5 161.2 162.4
Std Deviation (1e-4) 37.9 36.0 33.1 23.8 21.3 20.3 15.5 – 14.7 18.1 14.4 20.1 33.8 34.8 31.7 58.2 59.3 55.6 37.5 34.1 43.9
Intensity Change (Log2) – – – -0.25 -0.51 -1.22 -1.05 – -1.26 -1.2 -1.32 -1.26 0.1 0.04 -0.08 0.32 0.44 0.32 0.03 0.12 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 3.2 0 3.9 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.8 3.6 3.3 – 2.3 4.5 3.9 5.3 0 0 0 0 0 0
Cytoplasm -0.9 -5.2 -6.8 -9.2 – -9.1 -11.6 -9.5 -13.1 -3.2 -1.0 -0.6 -1.8 -0.7 -0.2
Endoplasmic Reticulum 0 4.6 4.7 8.5 – 7.7 9.3 7.1 10.4 0 0 0 4.7 3.6 4.7
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 4.3 4.3 4.8 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0