Standard name | |
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Description | Ribosomal 60S subunit protein L10; homologous to mammalian ribosomal protein L10 and bacterial L16; responsible for joining the 40S and 60S subunits; regulates translation initiation; similar to members of the QM gene family; protein abundance increases under DNA replication stress; mutations in human homolog implicated in T-cell acute lymphoblastic leukemia and also autism spectrum disorders (ASD); human RPL10 can complement yeast null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 8 | 3 | – | 6 | 11 | 10 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 4 | 0 | – | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 5 | 2 | 11 | 20 | 22 | – | 14 | 33 | 24 | 35 | 10 | 4 | 4 | 0 | 5 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 219 | 413 | 369 | 250 | 291 | 258 | 292 | – | 261 | 268 | 254 | 199 | 174 | 408 | 329 | 330 | 285 | 304 | 193 | 397 | 333 | 184 | 370 | 404 |
Endoplasmic Reticulum | 0 | 2 | 1 | 3 | 20 | 20 | 69 | – | 52 | 84 | 45 | 86 | 1 | 4 | 4 | 22 | 12 | 20 | 2 | 1 | 5 | 4 | 12 | 13 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 1 | 0 | 2 | 5 | – | 7 | 19 | 16 | 20 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 6 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 1 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 2 | 0 | 2 | 1 | 6 | – | 0 | 1 | 1 | 3 | 0 | 3 | 0 | 4 | 1 | 1 | 0 | 4 | 2 | 2 | 3 | 5 |
Unique Cell Count | 219 | 417 | 371 | 253 | 318 | 298 | 374 | 333 | 393 | 330 | 328 | 182 | 413 | 332 | 337 | 288 | 306 | 201 | 417 | 355 | 199 | 396 | 436 | |
Labelled Cell Count | 219 | 419 | 377 | 256 | 324 | 314 | 399 | 342 | 418 | 357 | 360 | 185 | 420 | 337 | 359 | 303 | 327 | 201 | 417 | 355 | 199 | 396 | 436 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 184.5 | 145.5 | 148.8 | 125.5 | 104.5 | 63.9 | 71.7 | – | 62.3 | 64.7 | 59.8 | 62.2 | 159.1 | 152.9 | 140.8 | 185.7 | 201.5 | 186.3 | 151.5 | 161.2 | 162.4 |
Std Deviation (1e-4) | 37.9 | 36.0 | 33.1 | 23.8 | 21.3 | 20.3 | 15.5 | – | 14.7 | 18.1 | 14.4 | 20.1 | 33.8 | 34.8 | 31.7 | 58.2 | 59.3 | 55.6 | 37.5 | 34.1 | 43.9 |
Intensity Change (Log2) | – | – | – | -0.25 | -0.51 | -1.22 | -1.05 | – | -1.26 | -1.2 | -1.32 | -1.26 | 0.1 | 0.04 | -0.08 | 0.32 | 0.44 | 0.32 | 0.03 | 0.12 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 3.2 | 0 | 3.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 1.8 | 3.6 | 3.3 | – | 2.3 | 4.5 | 3.9 | 5.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.9 | -5.2 | -6.8 | -9.2 | – | -9.1 | -11.6 | -9.5 | -13.1 | -3.2 | -1.0 | -0.6 | -1.8 | -0.7 | -0.2 |
Endoplasmic Reticulum | 0 | 4.6 | 4.7 | 8.5 | – | 7.7 | 9.3 | 7.1 | 10.4 | 0 | 0 | 0 | 4.7 | 3.6 | 4.7 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 4.3 | 4.3 | 4.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |