Standard name
Description Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex; involved with interaction partners Rsa4p and Ytm1p, in the ATP-dependent remodeling of the pre-60S particle at successive maturation steps during ribosomal biogenesis; involved in the removal of biogenesis factors including GTPase Nog2p prior to nuclear export; contains a hexameric AAA-motor head domain and a long flexible tail with a MIDAS (metal ion-dependent adhesion site) domain

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.47 0.33 0.25 0.17 0.17 0.11 0.07 0.07 0.05 0.12 0.05 0.38 0.32 0.27 0.48 0.37 0.38 0.49 0.38 0.41 0.22 0.3 0.19
Bud 0.06 0.06 0.11 0.11 0.12 0.13 0.11 0.1 0.13 0.09 0.1 0.1 0.07 0.13 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0.07 0.05 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.14 0.14 0.15 0.12 0.1 0.11 0.11 0.06 0.09 0.13 0.11 0.31 0.4 0.36 0.27 0.26 0.23 0.11 0.19 0.22 0.15 0.22 0.27
Cytoplasm 0.14 0.36 0.53 0.56 0.39 0.39 0.39 0.41 0.35 0.25 0.33 0.37 0.45 0.48 0.23 0.28 0.34 0 0 0 0.08 0 0
Endoplasmic Reticulum 0.38 0.23 0.06 0.05 0.14 0.07 0.08 0.06 0 0.05 0 0.1 0.07 0.11 0.06 0.05 0.05 0.26 0.13 0.11 0.25 0.25 0.33
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Mitochondria 0 0.07 0.08 0.23 0.24 0.43 0.42 0.52 0.63 0.64 0.62 0 0 0 0.06 0.07 0.08 0 0.06 0.09 0.14 0.09 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0.06 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 108 162 132 66 67 68 49 57 22 38 18 126 113 100 307 131 183 154 267 295 95 127 90
Bud 13 31 56 41 46 81 81 82 63 27 38 34 26 48 15 7 21 3 8 3 3 3 3
Bud Neck 0 0 16 2 2 2 0 3 0 0 0 3 1 1 0 0 2 8 76 53 31 21 21
Bud Site 3 6 3 5 7 15 7 14 5 2 8 1 1 0 1 0 0
Cell Periphery 32 72 79 45 41 69 75 48 43 41 39 104 138 134 174 91 114 34 136 159 65 93 132
Cytoplasm 31 177 278 216 154 247 276 340 166 77 124 124 157 177 145 98 165 3 15 21 34 11 15
Endoplasmic Reticulum 88 116 33 20 56 42 57 51 15 15 11 32 26 40 41 19 24 83 94 75 107 108 162
Endosome 1 2 2 0 8 6 14 4 1 0 1 0 1 1 6 3 3 3 15 9 2 5 5
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 0 0 17 18 10 6 8 3 0 2 2
Mitochondria 9 36 42 89 95 273 300 431 297 197 231 7 2 14 39 24 40 9 40 63 59 37 29
Nucleus 0 1 0 0 1 0 5 3 2 2 0 0 0 0 8 3 6 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 4 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 6 4 8 2 21 24 42 6 7 6 10 2 0 2 12 5 9 2 1 3 0 1 0
SpindlePole 6 11 22 3 8 18 21 13 3 1 9 3 5 2 36 27 20 0 0 0 0 0 1
Vac/Vac Membrane 8 7 4 0 7 1 14 26 10 4 10 1 0 0 15 18 16 3 31 16 26 12 14
Unique Cell Count 229 498 528 384 391 628 711 825 472 309 371 336 349 370 641 353 488 318 704 722 433 430 485
Labelled Cell Count 306 626 676 489 513 846 941 1082 635 410 499 437 470 519 817 444 614 318 704 722 433 430 485


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 5.5 4.5 4.1 4.7 4.4 4.4 4.3 4.0 4.1 4.0 5.5 6.1 5.4 8.4 8.6 8.0 5.8 6.2 6.6
Std Deviation (1e-4) 1.0 0.8 0.7 0.7 0.9 1.0 1.2 0.9 1.3 1.1 1.0 0.8 0.9 0.9 1.3 1.7 1.6 1.0 1.4 1.1
Intensity Change (Log2) -0.13 0.06 -0.03 -0.03 -0.09 -0.17 -0.14 -0.19 0.29 0.43 0.26 0.89 0.92 0.83 0.37 0.46 0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 3.9 2.4 0.7 8.0 3.8 4.3
Bud 0 0 0 0 0 0 0 0 -0.2 -1.6 1.1 -5.9 -4.9 -3.8
Bud Neck 0 0 0 0 0 0 0 0 -2.1 -2.9 -3.0 -4.4 -3.3 -3.2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 5.6 8.3 7.4 5.0 4.0 3.4
Cytoplasm 1.1 -4.0 -4.5 -4.8 -4.1 -5.6 -7.8 -5.7 -4.5 -2.2 -1.4 -10.6 -7.3 -6.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 1.8 0.7 2.5 0.1 -0.5 -0.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.8 5.2 0
Mitochondria 0 0 0 0 0 0 0 0 -3.6 -4.9 -2.5 -1.3 -0.6 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0.5 -0.1 0.4
SpindlePole 0 0 0 0 0 0 0 0 -2.8 -2.3 -3.3 1.1 2.2 -0.1
Vacuole 0 0 0 1.8 2.9 1.8 0 2.3 0 0 0 2.1 4.0 2.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rea1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available