Standard name
Description Acetyl-coA synthetase isoform; along with Acs1p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.15 0.1 0.06 0.06 0.06 0.06 0.07 0.1 0.05 0.05 0.05 0.05 0.05 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.11 0.09 0.11 0.12 0.11 0.15 0.09 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.91 0.93 0.91 0.8 0.68 0.69 0.63 0.59 0.58 0.53 0.62 0.89 0.85 0.81 0.91 0.85 0.85 0.8 0.7 0.59
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.1 0.07 0 0.12 0.16 0.35 0.33 0.38 0.43 0.47 0.56 0.42 0.1 0.14 0.12 0 0.05 0 0 0.1 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.08 0.14 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 3 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 2 0 0 1 0 0 0
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 79 19 12 13 14 21 25 19 13 12 15 13 16 37 0 1 0 3 4 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 0 0 0 1
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 1 0 0 1 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 0
Mitochondria 10 24 2 3 2 25 33 36 24 29 32 27 1 2 1 0 0 0 0 0 2
Nucleus 245 470 177 193 167 158 253 216 113 154 116 195 216 260 306 234 461 163 191 260 230
Nuclear Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 10 0 0 3 4
Nucleolus 26 35 5 26 33 81 122 130 82 124 123 132 23 42 46 8 27 7 10 36 53
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0
Vac/Vac Membrane 0 3 0 0 2 2 7 6 2 9 6 12 2 2 6 5 16 13 19 51 74
Unique Cell Count 257 516 190 212 208 232 365 341 192 265 220 314 242 305 378 258 546 192 239 372 387
Labelled Cell Count 288 613 204 234 218 281 436 414 242 329 289 383 257 326 397 258 546 192 239 372 387


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 104.3 144.8 111.0 99.9 94.9 91.9 88.7 83.7 76.4 74.1 65.1 68.0 113.7 118.0 111.6 99.8 103.2 110.1
Std Deviation (1e-4) 29.4 43.1 38.5 33.1 37.5 29.6 28.8 30.3 31.7 25.6 25.8 25.4 36.0 38.6 41.5 37.4 41.0 38.4
Intensity Change (Log2) -0.15 -0.23 -0.27 -0.32 -0.41 -0.54 -0.58 -0.77 -0.71 0.04 0.09 0.01 -0.15 -0.1 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -1.4 -1.5 -1.8 -1.1 0 -2.1 -1.7 -2.3 -1.8 -2.0 -0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 3.7 4.1 4.4 4.1 4.9 3.5 0 0 0
Nucleus -0.8 -3.7 -6.3 -6.4 -7.5 -7.8 -8.3 -9.0 -7.7 -1.4 -2.7 -3.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.6 4.5 8.2 8.2 9.0 9.3 10.3 11.6 9.6 2.9 4.1 3.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Acs2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available