Standard name
Description Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.13 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.19 0.34 0.31 0.24 0.31 0 0.07 0.05 0.05 0.07 0.06
Mitochondria 0.86 0.9 0.94 0.97 0.82 0.89 0.89 0.87 0.94 0.92 0.93 0.92 0.91 0.79 0.77 0.07 0.13 0.11 0.74 0.74 0.83 0.81 0.77 0.74
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0.05 0.05 0 0 0.07 0.12 0.1 0 0.09 0.11 0.11 0.06 0.07 0 0.05 0.13 0.07 0 0 0 0 0 0
Nucleolus 0.21 0 0 0 0.06 0.12 0.15 0.14 0.09 0.08 0.11 0.08 0.05 0 0 0.09 0.06 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0.06 0.16 0.11 0.18 0 0 0.05 0.06 0.06 0.07
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.13 0 0 0.09 0.05 0 0 0 0 0 0 0.05 0.09 0.09 0.14 0.17 0.22 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 1 0 1 2 1 2 1 1 1 3 2 0 3 4 3 0 4 5 3 8 18
Bud 2 3 3 4 9 11 7 11 14 11 10 15 2 1 4 2 6 3 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 1 0
Bud Site 1 0 1 0 0 1 2 6 3 2 2 5 0 0 0 1 0 0
Cell Periphery 2 12 4 6 3 5 4 3 2 4 5 9 1 0 6 9 7 2 0 0 0 0 0 1
Cytoplasm 0 6 1 2 13 6 7 6 3 8 2 6 0 4 4 3 6 1 0 1 0 0 0 0
Endoplasmic Reticulum 0 4 1 1 1 8 2 5 3 8 12 2 2 1 2 0 2 1 0 1 0 0 0 0
Endosome 0 4 3 2 6 0 2 7 1 0 1 4 2 6 1 1 1 1 8 11 3 6 12 8
Golgi 0 8 6 2 12 3 1 3 0 1 2 4 19 32 67 18 13 14 3 25 10 11 27 25
Mitochondria 87 293 202 230 239 419 379 660 362 479 434 589 162 134 153 4 7 5 90 251 181 177 327 305
Nucleus 2 1 0 0 1 2 4 4 0 1 0 1 0 1 1 2 1 0 0 2 0 0 0 0
Nuclear Periphery 3 15 11 5 8 34 53 77 15 48 50 70 10 12 8 3 7 3 3 2 0 0 6 6
Nucleolus 21 11 8 5 18 56 65 107 36 42 51 51 9 6 4 5 3 2 0 1 0 1 3 3
Peroxisomes 1 4 8 8 17 1 5 17 8 6 0 7 3 2 12 9 6 8 4 9 11 14 25 27
SpindlePole 1 1 0 1 0 4 3 5 3 2 3 6 1 1 1 2 2 0 2 1 0 1 0 1
Vac/Vac Membrane 6 44 7 3 27 23 10 11 11 10 6 13 9 15 18 8 9 10 5 23 0 2 8 10
Unique Cell Count 101 327 216 238 291 471 427 756 384 519 465 640 179 169 200 58 54 45 123 338 219 220 426 413
Labelled Cell Count 126 407 256 270 354 574 546 923 463 623 579 784 223 217 281 70 75 53 123 338 219 220 426 413


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 8.6 11.0 11.4 12.2 10.7 10.7 11.1 10.9 11.5 10.9 10.9 13.5 13.1 13.4 12.1 11.4 12.7 12.0 12.2 12.9
Std Deviation (1e-4) 3.0 2.3 2.6 2.6 3.5 3.2 3.1 3.6 3.5 4.1 4.0 3.7 3.0 3.1 2.5 3.0 3.0 3.2 2.7 3.0 3.4
Intensity Change (Log2) 0.05 0.14 -0.04 -0.04 0.0 -0.02 0.06 -0.02 -0.02 0.29 0.25 0.28 0.13 0.05 0.2 0.12 0.14 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1.2 0 0 0 1.6 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.7 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.8 0 0 0 0 0 0 0 3.2 5.3 8.2 6.8 0 0
Mitochondria 1.5 -3.8 -1.9 -1.9 -2.5 0.4 -0.6 -0.1 -0.7 -1.1 -4.2 -4.9 -13.6 -12.7 -12.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.7 -1.4 1.0 2.9 2.3 -0.7 1.9 2.4 2.5 0.2 0.8 -0.5 0 0 0
Nucleolus -1.0 1.3 3.4 4.3 4.2 2.6 2.2 3.1 2.1 0.6 -0.1 -1.0 0 0 0
Peroxisomes -0.2 1.1 0 0 -1.2 -1.2 0 0 0 0 0 1.1 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.7 1.0 -0.7 0 -0.3 0 0 -1.0 0.9 2.4 2.5 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mas1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available