Standard name
Description Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs; acts on large and small rRNAs, on snRNA U2, and on some mRNAs; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita; small nucleolar ribonucleoprotein particles are also known as snoRNPs

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0.05 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.72 0.59 0.43 0.51 0.47 0.48 0.31 0.28 0.33 0.38 0.33 0.39 0.58 0.58 0.5 0 0.11 0.06 0 0 0 0 0 0
Nucleus 0.09 0.16 0.06 0 0 0 0 0 0 0 0 0 0.07 0 0 0.08 0.1 0.12 0.07 0.12 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.93 0.94 0.93 0.97 0.94 0.94 0.91 0.92 0.9 0.91 0.85 0.92 0.93 0.92 0.87 0.94 0.89 0.87 0.88 0.84 0.85 0.9 0.88 0.84
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0.08 0.06 0 0.06 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 1 0 0 0 0 0 2 1 0 0 0 0 0 2 1 0 0 0 0 0 1 2 5 11
Bud Neck 1 0 3 1 1 0 0 0 0 2 3 3 0 1 1 0 0 0 1 1 2 2 9 13
Bud Site 0 0 0 1 0 1 5 6 4 7 5 11 0 3 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0
Cytoplasm 5 1 3 2 3 6 17 11 14 13 9 8 33 46 69 15 15 16 0 0 2 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 5 0 0 0 0 1 0
Endosome 0 0 0 0 0 1 2 2 0 0 1 0 4 7 9 0 0 1 1 0 0 2 3 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5 4
Mitochondria 238 140 50 80 102 130 128 137 103 169 74 131 422 590 578 20 31 14 0 0 2 5 6 16
Nucleus 29 39 7 2 9 7 5 12 11 13 10 10 50 45 49 35 29 28 23 27 5 13 10 26
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 2 1 0 1 0 0 0 0 0 0 0 2 2 6
Nucleolus 308 224 109 154 206 252 375 451 282 405 190 309 671 935 1000 426 259 204 291 194 111 550 699 802
Peroxisomes 2 0 1 3 1 2 8 5 2 3 2 1 2 6 12 2 2 2 0 0 0 1 3 5
SpindlePole 1 1 0 3 3 6 33 30 10 25 16 17 3 15 33 1 2 1 6 1 1 14 31 25
Vac/Vac Membrane 1 3 0 0 0 0 3 1 0 1 0 0 1 4 12 1 1 1 4 3 2 6 9 26
Unique Cell Count 331 238 117 158 218 269 412 488 314 447 223 337 724 1014 1150 454 292 235 332 232 132 609 797 958
Labelled Cell Count 586 408 173 246 325 405 578 656 426 639 312 491 1186 1656 1768 502 342 273 332 232 132 609 797 958


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.2 39.3 32.7 36.1 31.2 22.9 20.1 19.9 18.4 17.6 17.7 17.7 52.6 53.0 50.8 45.0 52.7 62.0 46.2 47.2 47.0
Std Deviation (1e-4) 18.3 12.3 11.6 13.0 11.3 7.6 7.9 8.7 7.9 7.4 9.0 6.8 21.1 24.3 25.5 19.7 33.3 35.1 16.6 18.6 19.5
Intensity Change (Log2) 0.14 -0.07 -0.51 -0.7 -0.72 -0.83 -0.89 -0.88 -0.89 0.69 0.7 0.64 0.46 0.69 0.92 0.5 0.53 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 1.0 1.0 1.5 0 1.1 1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.3 0.7 1.0 -2.4 -3.1 -1.9 -1.0 -1.7 -0.7 3.1 3.2 1.6 -11.3 -7.4 -8.4
Nucleus 0 -0.8 0 0 0 0 0 -0.6 0 0.4 -0.8 -0.9 0.6 1.3 1.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.7 0.5 0.2 -0.7 -0.3 -1.1 -0.9 -2.1 -0.5 -0.2 -0.4 -1.9 0.3 -1.4 -1.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3.2 2.8 0 2.6 3.0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cbf5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available