Standard name
Human Ortholog
Description eIF3j component of translation initiation factor 3 (eIF3); dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of eIF3; required for 20S pre-rRNA processing; required at post-transcriptional step for efficient retrotransposition; absence decreases Ty1 Gag:GFP protein levels; binds eIF3 subunits Rpg1p, Prt1p and 18S rRNA; eIF3 also involved in programmed stop codon read through; human homolog EIF3J can complement yeast hcr1 mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0.06 0.05 0.07 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0.07 0.06 0.06 0.06 0.06 0.05 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.98 0.95 0.91 0.93 0.83 0.8 0.71 0.7 0.68 0.71 0.73 0.97 0.91 0.94 0.96 0.93 0.97 0.99 0.9 0.88 0.9 0.95 0.85
Endoplasmic Reticulum – 0 0.05 0.05 0.06 0.08 0.15 0.19 0.12 0.19 0.07 0.09 0 0.07 0.05 0 0.05 0 0 0 0.06 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0.1 0.08 0.11 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 1 1 4 8 19 9 15 13 14 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 1 0 0 0 0 1 0 3 0 1 1 0 0 0 0 0 0 0 0 1 0 1 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 1 1 4 13 14 15 20 11 11 15 21 0 0 3 4 5 2 0 0 0 0 0 1
Cytoplasm – 108 117 118 177 189 198 241 124 138 112 145 28 107 134 199 145 153 23 111 114 69 126 115
Endoplasmic Reticulum – 2 6 6 11 19 37 64 21 38 11 17 1 8 7 6 8 4 0 5 7 2 3 5
Endosome – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 3 0 9 9 5 6 5 0 0 0 4 6 3 0 0 0 0 0 0
Mitochondria – 1 0 3 0 4 1 8 17 16 17 21 0 1 0 5 3 1 0 2 2 0 0 0
Nucleus – 0 0 1 0 1 2 3 1 0 2 2 0 3 1 0 1 0 0 0 0 1 0 2
Nuclear Periphery – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 1 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 2 1 0 7 3 10 6 7 6 3 0 0 1 3 5 2 0 1 1 2 0 5
Unique Cell Count 110 123 129 190 228 248 338 176 202 157 198 29 117 143 208 156 158 24 124 129 77 133 137
Labelled Cell Count 114 126 134 202 241 265 374 203 231 183 231 29 119 146 221 173 165 24 124 129 77 133 137


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 22.0 29.9 28.9 29.3 24.6 23.2 21.3 19.0 18.7 18.8 18.0 35.9 35.6 37.6 19.9 27.6 28.4 32.4 36.0 32.7
Std Deviation (1e-4) – 6.0 6.6 6.6 5.6 5.4 5.4 4.8 5.1 4.6 5.0 4.2 6.4 10.9 9.1 5.7 11.5 6.8 9.3 7.4 10.7
Intensity Change (Log2) – – – -0.05 -0.03 -0.28 -0.37 -0.49 -0.65 -0.68 -0.67 -0.73 0.26 0.25 0.33 -0.59 -0.11 -0.07 0.12 0.27 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.7 0 3.1 3.3 3.0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2.5 2.4 2.3 2.3 0 0 3.1 3.4 0 0 0 0 0 0
Cytoplasm -1.2 -0.7 -3.3 -3.9 -5.4 -5.3 -5.7 -5.1 -4.9 0.3 -1.1 -0.5 0.2 -0.8 0.7
Endoplasmic Reticulum -0.1 0.3 1.2 2.8 3.7 2.1 3.6 0.7 1.3 0 0.6 0 0 0.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.5 3.2 3.8 3.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.2053 35.5454 31.877 30.6242 28.4422 31.6094 28.4502 42.688 37.5639 34.5215 31.8257 37.4938 24.6882 37.3582 35.243 33.9706 31.169 34.4992
Actin 0.007 0.0013 0.0064 0.0208 0.0006 0.0043 0.0106 0.0005 0.0036 0.0053 0.0118 0.0142 0.0142 0.0025 0.0022 0.0003 0.0006 0.0036
Bud 0.0012 0.0007 0.0019 0.0043 0.0005 0.0012 0.0014 0.0003 0.0008 0.001 0.001 0.0005 0.0009 0.0004 0.001 0.0011 0.0005 0.0004
Bud Neck 0.0011 0.0007 0.0012 0.0011 0.0008 0.0075 0.0009 0.0002 0.0005 0.0015 0.0005 0.0013 0.0006 0.0006 0.0006 0.001 0.0004 0.0022
Bud Periphery 0.0017 0.0007 0.0027 0.0038 0.0006 0.002 0.0011 0.0001 0.0005 0.0006 0.0006 0.0007 0.0006 0.0002 0.0003 0.0004 0.0001 0.0001
Bud Site 0.0015 0.0008 0.0018 0.0021 0.0002 0.001 0.0024 0.0004 0.0024 0.0374 0.001 0.0002 0.001 0.0026 0.0013 0.0003 0.0005 0.0001
Cell Periphery 0.0068 0.0031 0.0033 0.0028 0.0047 0.0026 0.0021 0.0003 0.001 0.0012 0.0002 0.0006 0.0014 0.001 0.0006 0.0083 0.0001 0.0002
Cytoplasm 0.6569 0.906 0.7835 0.6798 0.7262 0.77 0.7833 0.9733 0.9076 0.7922 0.8614 0.8727 0.765 0.9497 0.9143 0.8221 0.9017 0.9259
Cytoplasmic Foci 0.0227 0.0025 0.0159 0.0096 0.0114 0.0154 0.0184 0.0021 0.0118 0.0043 0.0172 0.0065 0.0213 0.0022 0.0079 0.0044 0.0248 0.0059
Eisosomes 0.0001 0 0.0001 0.0002 0 0 0.0003 0 0 0.0001 0 0.0001 0.0001 0 0.0001 0 0 0
Endoplasmic Reticulum 0.1067 0.0497 0.0434 0.0092 0.1064 0.0961 0.051 0.012 0.0129 0.007 0.0215 0.0649 0.0675 0.0163 0.0103 0.0109 0.0086 0.0319
Endosome 0.0503 0.0049 0.0255 0.0306 0.0693 0.0453 0.0417 0.0027 0.0165 0.0138 0.04 0.0173 0.0266 0.0035 0.0207 0.0159 0.0204 0.0119
Golgi 0.0063 0.0016 0.0035 0.0026 0.0043 0.011 0.0063 0.0006 0.0045 0.0052 0.0081 0.0037 0.0035 0.001 0.002 0.0003 0.0007 0.0019
Lipid Particles 0.0128 0.0003 0.0024 0.0107 0.0081 0.003 0.0093 0.0001 0.0009 0.0011 0.0025 0.0002 0.007 0.0007 0.0014 0.0007 0.0013 0.0002
Mitochondria 0.006 0.0039 0.0191 0.0192 0.0079 0.0079 0.0059 0.0001 0.0104 0.0405 0.0018 0.0047 0.0014 0.0019 0.0023 0.0001 0.0008 0.0013
None 0.0033 0.0002 0.0034 0.0043 0.0008 0.0006 0.0068 0.0001 0.0009 0.0004 0.0035 0.0005 0.0088 0.0002 0.0055 0.0002 0.0025 0.0003
Nuclear Periphery 0.0236 0.0018 0.0045 0.0111 0.0088 0.0066 0.008 0.0006 0.0026 0.0091 0.0029 0.0022 0.0121 0.0038 0.0022 0.0065 0.003 0.0036
Nucleolus 0.0004 0 0.0003 0.0006 0.0001 0.0001 0.0004 0 0 0.0002 0.0001 0 0.0003 0 0.0002 0.0001 0.0004 0
Nucleus 0.0064 0.0009 0.0019 0.0052 0.002 0.0019 0.0029 0.0005 0.001 0.0018 0.0011 0.0008 0.0044 0.0013 0.0016 0.0029 0.0024 0.0035
Peroxisomes 0.0018 0 0.0011 0.0028 0.0001 0.0006 0.0032 0 0.0007 0.0004 0.0002 0.0001 0.0024 0.0006 0.0003 0 0.0003 0.0001
Punctate Nuclear 0.003 0 0.0011 0.0026 0.0001 0.0006 0.0017 0 0.0003 0.0002 0.0006 0.0001 0.0033 0.0001 0.0004 0.0001 0.0013 0.0001
Vacuole 0.0702 0.0189 0.067 0.1489 0.0353 0.0157 0.037 0.0054 0.0171 0.0642 0.0196 0.0064 0.0528 0.0105 0.0214 0.1135 0.024 0.0051
Vacuole Periphery 0.0105 0.002 0.0101 0.0276 0.0119 0.0065 0.0055 0.0006 0.0038 0.0126 0.0045 0.0023 0.0048 0.0009 0.0033 0.0109 0.0056 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 195.6538 140.9109 155.0085 144.7215 168.5182 207.292 163.4087 177.1385 204.5235 178.6339
Translational Efficiency 2.6287 3.0079 2.5078 3.1246 2.5639 2.655 2.3003 2.1905 1.9732 2.2183

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘