Standard name
Human Ortholog
Description Structural component of 3 complexes; subunit of the Nup84p nuclear pore subcomplex that contributes to nucleocytoplasmic transport and NPC biogenesis; subunit of the COPII vesicle coat required for ER-to-Golgi transport; subunit of SEACAT, a subcomplex of the coatomer-related, vacuolar-associated SEA complex, that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p, thereby resulting in activation of TORC1 signaling; human SEC13 homolog
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.26 0.39 0.4 0.43 0.4 0.33 0.17 0.28 – 0.27 0.27 0.26 0.48 0.29 0.23 0.33 0.4 0.33 0.45 0.5 0.6 0.44 0.48 0.38
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.06 0 0 0 0.05 0 0.07 – 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.37 0.78 0.52 0.53 0.64 0.5 0.48 0.41 – 0.25 0.25 0.25 0.67 0.41 0.38 0 0.07 0 0.14 0.28 0.12 0.12 0.11 0.17
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.32 0.43 0.66 0.72 0.67 0.34 0.4 0.38 – 0.34 0.25 0.34 0.56 0.54 0.77 0.96 0.9 0.91 0.37 0.18 0.25 0.32 0.35 0.35
Mitochondria 0.42 0.05 0.48 0.42 0.36 0.56 0.6 0.6 – 0.73 0.87 0.8 0 0.27 0 0.14 0.13 0.11 0 0 0 0 0 0
Nucleus 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 84 42 46 51 59 35 57 – 44 44 49 23 12 13 51 67 46 26 114 68 31 56 47
Bud 0 4 2 2 4 7 3 2 – 5 8 6 0 0 1 6 6 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 3 2 6 0 1 5 – 4 2 5 1 1 0 2 3 2 0 0 1 0 0 0
Cytoplasm 2 12 0 3 4 8 7 14 – 2 2 4 2 2 3 1 4 5 0 1 0 1 0 0
Endoplasmic Reticulum 7 167 55 56 81 89 97 83 – 40 42 47 32 17 21 4 11 6 8 64 13 8 13 21
Endosome 0 0 0 0 0 0 2 2 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 6 91 70 76 85 61 80 78 – 55 41 63 27 22 43 149 149 129 21 42 28 22 41 43
Mitochondria 8 10 51 44 45 99 121 122 – 119 143 150 0 11 2 22 21 15 0 1 0 2 1 1
Nucleus 1 0 0 1 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 3 2 – 3 3 4 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 1 0 0 0 0 1 0 0 – 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 0 0 0 1 1 – 4 0 1 2 1 3 2 2 1 0 0 0 0 1 2
Unique Cell Count 19 214 106 106 126 177 202 203 162 165 187 48 41 56 155 166 141 60 230 114 72 118 125
Labelled Cell Count 30 373 224 230 276 325 350 366 278 285 334 87 67 86 237 263 205 60 230 114 72 118 125


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.1 32.6 22.3 21.8 20.9 19.1 17.9 18.1 – 17.7 17.3 17.0 33.7 34.5 33.6 41.1 40.6 42.2 33.8 35.4 37.1
Std Deviation (1e-4) 9.5 6.0 4.6 4.7 3.8 4.0 3.9 3.9 – 4.1 3.8 4.0 9.6 13.5 10.7 9.3 9.6 10.1 12.4 7.5 10.9
Intensity Change (Log2) – – – -0.04 -0.09 -0.22 -0.32 -0.3 – -0.33 -0.37 -0.4 0.59 0.63 0.59 0.88 0.86 0.92 0.6 0.66 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 0.1 -1.1 -4.3 -2.1 – -2.1 -2.2 -2.4 1.0 -1.2 -2.1 -1.1 0.1 -1.1
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.1 1.9 -0.3 -0.6 -1.8 – -4.6 -4.4 -4.6 1.7 -1.1 -1.7 -9.4 -8.5 -8.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0.9 0.2 -5.2 -4.4 -4.6 – -5.1 -6.7 -5.3 -1.2 -1.4 1.4 6.5 4.8 5.0
Mitochondria -1.0 -1.9 1.3 2.0 2.0 – 4.2 6.9 5.7 -5.9 -2.3 -5.7 -6.0 -6.5 -6.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 32.7715 33.2064 33.4025 32.399 31.0112 33.303 36.0193 37.054 36.281 35.7569 34.1429 36.7227 34.9262 35.7214 33.8445 34.3966 34.0792 33.8679
Actin 0.0462 0.014 0.0161 0.0043 0.0046 0.0111 0.0351 0.0082 0.0274 0.0069 0.0072 0.0099 0.042 0.007 0.0124 0.004 0.0042 0.0086
Bud 0 0.0001 0 0 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0
Bud Neck 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0008 0.0002 0.0001 0.0001 0.0004 0 0.0001 0.0001 0 0.0001
Bud Periphery 0.0001 0.0001 0 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0 0 0 0
Bud Site 0.0001 0.0001 0 0 0 0 0.0001 0 0.0011 0 0 0 0.0002 0 0 0 0 0
Cell Periphery 0.0003 0.0005 0.0004 0.0002 0.0002 0.0002 0.0003 0.0002 0.0003 0.0005 0.0003 0.0003 0.0005 0.0002 0.0002 0.0003 0.0004 0.0003
Cytoplasm 0.0001 0.0007 0.0001 0 0 0 0.0003 0.0001 0.0003 0.0001 0.0011 0.0001 0.0007 0.0001 0.0001 0.0001 0 0.0001
Cytoplasmic Foci 0.0018 0.005 0.0016 0.0005 0.0006 0.0005 0.004 0.0014 0.0029 0.0009 0.0022 0.0007 0.0021 0.0011 0.001 0.0007 0.0004 0.0006
Eisosomes 0.0084 0.0029 0.0072 0.0024 0.0034 0.0109 0.0056 0.0012 0.0108 0.0131 0.0065 0.0081 0.0082 0.0018 0.0027 0.0032 0.0023 0.0071
Endoplasmic Reticulum 0.0069 0.0079 0.0084 0.0156 0.0048 0.0096 0.0085 0.0099 0.009 0.0138 0.0045 0.0119 0.0046 0.0066 0.0111 0.015 0.0309 0.0157
Endosome 0.0086 0.0136 0.009 0.0027 0.0048 0.0045 0.0102 0.006 0.0064 0.0058 0.0072 0.0048 0.0067 0.0052 0.005 0.0066 0.0019 0.0045
Golgi 0.9109 0.9241 0.9468 0.9728 0.9791 0.9603 0.9012 0.9681 0.9288 0.9419 0.9317 0.9532 0.9117 0.9705 0.9569 0.9647 0.9473 0.9586
Lipid Particles 0.0085 0.0233 0.0068 0.0008 0.0016 0.0013 0.0164 0.0032 0.0077 0.0155 0.0038 0.0026 0.011 0.0061 0.0076 0.0031 0.0112 0.003
Mitochondria 0.0032 0.0006 0.0011 0.0001 0.0002 0.0002 0.0073 0.0006 0.0004 0.0003 0.0179 0.0018 0.0051 0.0002 0.0017 0.0003 0.0001 0.0002
None 0.0001 0.0003 0 0 0 0 0.0002 0 0.0004 0 0.0061 0 0.0017 0 0 0 0 0
Nuclear Periphery 0.0026 0.0019 0.0012 0.0003 0.0002 0.0007 0.0041 0.0006 0.0011 0.0004 0.0063 0.0054 0.0014 0.0005 0.0005 0.0008 0.001 0.0008
Nucleolus 0 0.0005 0 0 0 0 0.0001 0 0 0 0.0002 0 0.0003 0 0 0 0 0
Nucleus 0.0001 0.0003 0 0 0 0 0.0002 0 0.0001 0 0.0006 0 0.0003 0 0 0 0 0
Peroxisomes 0.0002 0.0001 0 0 0 0 0.0006 0 0.0007 0 0.0002 0 0.0003 0 0 0 0 0
Punctate Nuclear 0.0001 0.0002 0 0 0 0 0.0001 0 0.0013 0 0.0008 0 0.0003 0 0 0 0 0
Vacuole 0.0002 0.0016 0.0001 0 0 0.0001 0.0005 0.0001 0.0001 0.0001 0.0008 0.0001 0.0003 0 0.0001 0.0001 0.0001 0
Vacuole Periphery 0.0014 0.0022 0.001 0.0002 0.0003 0.0005 0.0053 0.0003 0.0004 0.0003 0.0023 0.0009 0.0019 0.0006 0.0005 0.001 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 165.3872 130.1928 146.5505 139.3269 140.0208 152.9602 122.7132 129.8818 150.1365 141.2099
Translational Efficiency 1.3475 1.6724 1.5306 1.8491 1.5999 1.6497 1.9263 1.6393 1.5773 1.5013

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Structural component of 3 complexes; subunit of the Nup84p nuclear pore subcomplex that contributes to nucleocytoplasmic transport and NPC biogenesis; subunit of the COPII vesicle coat required for ER-to-Golgi transport; subunit of SEACAT, a subcomplex of the coatomer-related, vacuolar-associated SEA complex, that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p, thereby resulting in activation of TORC1 signaling; human SEC13 homolog
Localization
Cell Percentages ER (76%)
Cell Cycle Regulation No
Subcompartmental Group ER-1

Sec13

Sec13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sec13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available