Standard name
Description Structural component of 3 complexes; subunit of the Nup84p nuclear pore subcomplex that contributes to nucleocytoplasmic transport and NPC biogenesis; subunit of the COPII vesicle coat required for ER-to-Golgi transport; subunit of SEACAT, a subcomplex of the coatomer-related, vacuolar-associated SEA complex, that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p, thereby resulting in activation of TORC1 signaling; human SEC13 homolog
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ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.26 0.39 0.4 0.43 0.4 0.33 0.17 0.28 – 0.27 0.27 0.26 0.48 0.29 0.23 0.33 0.4 0.33 0.45 0.5 0.6 0.44 0.48 0.38
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.06 0 0 0 0.05 0 0.07 – 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.37 0.78 0.52 0.53 0.64 0.5 0.48 0.41 – 0.25 0.25 0.25 0.67 0.41 0.38 0 0.07 0 0.14 0.28 0.12 0.12 0.11 0.17
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.32 0.43 0.66 0.72 0.67 0.34 0.4 0.38 – 0.34 0.25 0.34 0.56 0.54 0.77 0.96 0.9 0.91 0.37 0.18 0.25 0.32 0.35 0.35
Mitochondria 0.42 0.05 0.48 0.42 0.36 0.56 0.6 0.6 – 0.73 0.87 0.8 0 0.27 0 0.14 0.13 0.11 0 0 0 0 0 0
Nucleus 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 84 42 46 51 59 35 57 – 44 44 49 23 12 13 51 67 46 26 114 68 31 56 47
Bud 0 4 2 2 4 7 3 2 – 5 8 6 0 0 1 6 6 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 3 2 6 0 1 5 – 4 2 5 1 1 0 2 3 2 0 0 1 0 0 0
Cytoplasm 2 12 0 3 4 8 7 14 – 2 2 4 2 2 3 1 4 5 0 1 0 1 0 0
Endoplasmic Reticulum 7 167 55 56 81 89 97 83 – 40 42 47 32 17 21 4 11 6 8 64 13 8 13 21
Endosome 0 0 0 0 0 0 2 2 – 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 6 91 70 76 85 61 80 78 – 55 41 63 27 22 43 149 149 129 21 42 28 22 41 43
Mitochondria 8 10 51 44 45 99 121 122 – 119 143 150 0 11 2 22 21 15 0 1 0 2 1 1
Nucleus 1 0 0 1 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 3 2 – 3 3 4 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 1 0 0 0 0 1 0 0 – 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 1 0 0 0 1 1 – 4 0 1 2 1 3 2 2 1 0 0 0 0 1 2
Unique Cell Count 19 214 106 106 126 177 202 203 162 165 187 48 41 56 155 166 141 60 230 114 72 118 125
Labelled Cell Count 30 373 224 230 276 325 350 366 278 285 334 87 67 86 237 263 205 60 230 114 72 118 125


ER to Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.1 32.6 22.3 21.8 20.9 19.1 17.9 18.1 – 17.7 17.3 17.0 33.7 34.5 33.6 41.1 40.6 42.2 33.8 35.4 37.1
Std Deviation (1e-4) 9.5 6.0 4.6 4.7 3.8 4.0 3.9 3.9 – 4.1 3.8 4.0 9.6 13.5 10.7 9.3 9.6 10.1 12.4 7.5 10.9
Intensity Change (Log2) – – – -0.04 -0.09 -0.22 -0.32 -0.3 – -0.33 -0.37 -0.4 0.59 0.63 0.59 0.88 0.86 0.92 0.6 0.66 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.6 0.1 -1.1 -4.3 -2.1 – -2.1 -2.2 -2.4 1.0 -1.2 -2.1 -1.1 0.1 -1.1
Bud 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.1 1.9 -0.3 -0.6 -1.8 – -4.6 -4.4 -4.6 1.7 -1.1 -1.7 -9.4 -8.5 -8.6
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Golgi 0.9 0.2 -5.2 -4.4 -4.6 – -5.1 -6.7 -5.3 -1.2 -1.4 1.4 6.5 4.8 5.0
Mitochondria -1.0 -1.9 1.3 2.0 2.0 – 4.2 6.9 5.7 -5.9 -2.3 -5.7 -6.0 -6.5 -6.6
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0