Standard name
Description Endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0.07 0.06 0.12 0.07 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0.16 0.18 0.14 0.39 0.43 0.47 0.24 0.07 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0.91 0.89 0.93 0.94 0.94 0.91 0.91 0.91 0.84 0.81 0.83 0.85 0.92 0.91 0.82 0.86 0.84 0.86 0.9 0.85 0.9 0.73 0.67 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.06 0 0 0 0 0 0.06 0.13 0.09 0.16 0.05 0.06 0.06 0.11 0 0 0.06 0 0 0 0.1 0.15 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.06 0 0.06 0.08 0.13
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0
Bud 0 1 0 0 1 1 2 4 1 4 3 3 0 0 0 1 0 0 0 1 1 1 3 5
Bud Neck 0 0 0 0 3 0 0 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 0 0 0 0 0 2 8 4 10 10 11 12 0 0 0 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 10 13 10 11 14 14 10 8 6 6 8 17 16 43 18 13 6 1 2 0 0 1 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 3 3 4 0 0 0 0 0 2
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 2 2 1 1 4 0 1 2 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 1 0 1 0 2
Mitochondria 6 22 8 5 4 44 58 43 111 100 116 68 16 5 4 8 4 10 0 2 2 6 4 5
Nucleus 131 204 238 185 259 257 291 286 239 189 207 246 226 242 282 229 148 164 128 208 231 120 192 185
Nuclear Periphery 1 1 0 0 0 1 1 1 2 5 1 2 2 3 4 3 0 1 0 1 1 0 0 1
Nucleolus 17 13 1 2 3 9 12 18 36 22 41 15 15 16 37 9 7 11 6 4 6 15 43 28
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 2 2 2 4 2 4 4 0 1 4 1 2 3 0 0 1 1 1 0 2 4 5
Vac/Vac Membrane 1 6 4 0 2 1 1 0 2 0 2 1 0 2 5 7 8 12 1 13 8 10 23 37
Unique Cell Count 144 228 257 197 275 281 320 313 285 233 249 288 245 266 346 266 177 191 143 245 257 165 288 296
Labelled Cell Count 165 258 266 204 286 333 389 372 413 336 389 359 278 286 386 281 187 210 143 245 257 165 288 296


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.5 6.8 6.4 6.4 5.1 5.2 5.5 4.4 4.3 4.1 4.8 6.8 7.1 6.8 9.6 9.8 9.2 6.3 6.5 6.8
Std Deviation (1e-4) 1.5 1.2 1.2 1.1 1.3 1.1 1.4 1.1 0.9 1.1 1.0 1.0 1.6 1.7 2.1 2.4 2.2 2.2 1.5 1.9 1.6
Intensity Change (Log2) -0.09 -0.08 -0.41 -0.39 -0.31 -0.63 -0.65 -0.74 -0.49 0.01 0.07 0.0 0.51 0.53 0.44 -0.1 -0.06 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 -0.6 0 -0.4 -1.1 -1.4 -1.4 -1.6 -1.4 0.9 0.5 3.1 0.8 1.0 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.4 -1.3 4.9 5.6 4.4 0 0 0 0 1.8 -0.9 -1.7 -0.1 0 1.1
Nucleus 0.5 0.7 -0.5 -0.7 -0.5 -3.1 -3.8 -3.3 -2.7 -0.2 -0.7 -3.9 -2.4 -2.9 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.7 3.5 0 0 0 0 3.7 3.6 5.1 0 0 3.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0.9 0 2.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ysh1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available