Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 0 | 0 | 9 | – | 35 | 17 | 33 | 15 | 21 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 0 | 4 | 0 | 0 | 1 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 1 | 1 | 7 | 10 | – | 23 | 24 | 34 | 14 | 22 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Cytoplasm | 252 | 134 | 276 | 232 | 338 | 376 | – | 488 | 244 | 271 | 172 | 211 | 67 | 132 | 137 | 56 | 68 | 73 | 241 | 122 | 284 | 41 | 88 | 92 |
Endoplasmic Reticulum | 1 | 6 | 9 | 0 | 34 | 20 | – | 149 | 62 | 83 | 43 | 60 | 4 | 4 | 2 | 6 | 1 | 5 | 1 | 3 | 13 | 0 | 2 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 2 | 1 | 0 | 1 | 4 | – | 22 | 18 | 8 | 4 | 16 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4 | 4 | 2 | 18 | – | 54 | 84 | 57 | 74 | 92 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 0 | 1 | 0 | – | 13 | 12 | 10 | 2 | 5 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 1 | 2 | 0 | 1 | 1 |
Unique Cell Count | 253 | 139 | 291 | 235 | 372 | 415 | 706 | 384 | 420 | 270 | 358 | 70 | 135 | 138 | 59 | 70 | 74 | 250 | 133 | 311 | 45 | 98 | 103 | |
Labelled Cell Count | 258 | 143 | 299 | 237 | 384 | 438 | 786 | 462 | 499 | 326 | 427 | 72 | 137 | 141 | 69 | 73 | 78 | 250 | 133 | 311 | 45 | 98 | 103 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 59.8 | 69.2 | 38.3 | 47.3 | 38.5 | 28.4 | – | 23.0 | 21.3 | 21.2 | 20.2 | 20.8 | 58.3 | 55.6 | 54.7 | 85.0 | 59.3 | 84.4 | 60.1 | 67.4 | 64.1 |
Std Deviation (1e-4) | 13.3 | 15.6 | 10.1 | 10.0 | 8.6 | 5.1 | – | 5.1 | 5.1 | 4.7 | 4.3 | 4.8 | 14.1 | 11.0 | 8.8 | 32.3 | 24.2 | 29.2 | 12.6 | 16.8 | 16.7 |
Intensity Change (Log2) | – | – | – | 0.31 | 0.01 | -0.43 | – | -0.74 | -0.85 | -0.86 | -0.92 | -0.88 | 0.61 | 0.54 | 0.51 | 1.15 | 0.63 | 1.14 | 0.65 | 0.82 | 0.74 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 3.2 | 2.9 | 4.3 | 3.4 | 3.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 2.7 | 4.0 | 4.7 | 3.6 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.4 | -1.9 | -2.1 | – | -8.7 | -9.6 | -9.4 | -9.2 | -10.5 | 0.3 | 1.4 | 2.3 | 0 | 0.8 | 1.4 |
Endoplasmic Reticulum | 0 | 3.1 | 1.1 | – | 7.1 | 5.5 | 6.5 | 5.2 | 5.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | – | 2.7 | 3.4 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 2.2 | – | 3.8 | 7.8 | 5.7 | 8.9 | 8.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | – | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 60.082 | 67.4418 | 65.4626 | 56.5814 | 66.3324 | 62.1582 | 57.3077 | 71.6949 | 61.9054 | 59.1343 | 57.1316 | 65.9236 | 60.875 | 75.6579 | 66.9925 | 70.5389 | 63.096 | 65.715 |
Actin | 0.0013 | 0.0008 | 0.0016 | 0.005 | 0.0005 | 0.0023 | 0.0024 | 0.0003 | 0.0117 | 0.0187 | 0.0004 | 0.0027 | 0.0008 | 0.0003 | 0.0013 | 0.0003 | 0.001 | 0.0085 |
Bud | 0.0009 | 0.0005 | 0.0011 | 0.0009 | 0.0004 | 0.0014 | 0.0016 | 0.0003 | 0.0016 | 0.0011 | 0.0004 | 0.0021 | 0.0014 | 0.0005 | 0.0009 | 0.0028 | 0.0015 | 0.0014 |
Bud Neck | 0.0008 | 0.0006 | 0.0008 | 0.0023 | 0.0007 | 0.0017 | 0.0011 | 0.0004 | 0.0007 | 0.0018 | 0.0005 | 0.0016 | 0.0006 | 0.0003 | 0.0004 | 0.0006 | 0.0008 | 0.0013 |
Bud Periphery | 0.0015 | 0.0005 | 0.0017 | 0.0007 | 0.0002 | 0.0018 | 0.0012 | 0.0002 | 0.0012 | 0.0009 | 0.0002 | 0.0027 | 0.0017 | 0.0003 | 0.0009 | 0.0031 | 0.0014 | 0.0022 |
Bud Site | 0.0005 | 0.0003 | 0.0005 | 0.0007 | 0.0001 | 0.0005 | 0.0077 | 0.0003 | 0.0033 | 0.0043 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0004 |
Cell Periphery | 0.0144 | 0.0091 | 0.0101 | 0.003 | 0.0034 | 0.0025 | 0.0033 | 0.0039 | 0.004 | 0.0005 | 0.0001 | 0.0024 | 0.003 | 0.0013 | 0.0021 | 0.0009 | 0.0011 | 0.0023 |
Cytoplasm | 0.7362 | 0.894 | 0.8319 | 0.7655 | 0.8962 | 0.7967 | 0.7967 | 0.9434 | 0.8131 | 0.7708 | 0.8497 | 0.8316 | 0.7881 | 0.9719 | 0.8872 | 0.8443 | 0.8283 | 0.8066 |
Cytoplasmic Foci | 0.0021 | 0.0019 | 0.003 | 0.0058 | 0.0065 | 0.0052 | 0.0093 | 0.0014 | 0.0184 | 0.0255 | 0.0308 | 0.0077 | 0.0111 | 0.0015 | 0.0043 | 0.004 | 0.0105 | 0.0107 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0005 | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0931 | 0.0414 | 0.0463 | 0.0369 | 0.0149 | 0.0969 | 0.0421 | 0.0244 | 0.0379 | 0.0042 | 0.003 | 0.0305 | 0.0567 | 0.0064 | 0.0196 | 0.0064 | 0.0047 | 0.0576 |
Endosome | 0.0097 | 0.0043 | 0.0099 | 0.0146 | 0.0237 | 0.0252 | 0.0203 | 0.0025 | 0.0147 | 0.0526 | 0.0558 | 0.0331 | 0.0193 | 0.0027 | 0.0116 | 0.0261 | 0.0214 | 0.0317 |
Golgi | 0.0029 | 0.0011 | 0.0015 | 0.0046 | 0.004 | 0.0069 | 0.0019 | 0.0004 | 0.0043 | 0.006 | 0.0071 | 0.0106 | 0.0025 | 0.0007 | 0.0014 | 0.0016 | 0.0022 | 0.011 |
Lipid Particles | 0.001 | 0.0018 | 0.001 | 0.0087 | 0.0011 | 0.0008 | 0.0028 | 0.0003 | 0.0059 | 0.0055 | 0.0052 | 0.0006 | 0.004 | 0.0001 | 0.0008 | 0.0001 | 0.0159 | 0.0056 |
Mitochondria | 0.0047 | 0.0022 | 0.0048 | 0.0038 | 0.0004 | 0.0048 | 0.007 | 0.0003 | 0.0088 | 0.0018 | 0.0106 | 0.0119 | 0.0171 | 0.0005 | 0.0067 | 0.0022 | 0.0088 | 0.0075 |
None | 0.0014 | 0.0002 | 0.0006 | 0.001 | 0.0002 | 0.0015 | 0.0036 | 0.0001 | 0.0044 | 0.0023 | 0.0003 | 0.0036 | 0.0032 | 0.0002 | 0.0032 | 0.0003 | 0.0047 | 0.0019 |
Nuclear Periphery | 0.0183 | 0.0032 | 0.0073 | 0.004 | 0.0024 | 0.0064 | 0.0159 | 0.0015 | 0.0039 | 0.0044 | 0.0018 | 0.0047 | 0.011 | 0.0009 | 0.0039 | 0.0033 | 0.0072 | 0.0043 |
Nucleolus | 0.0018 | 0.0001 | 0.0003 | 0.0007 | 0.0001 | 0.0007 | 0.0014 | 0.0001 | 0.0009 | 0.0005 | 0 | 0.0001 | 0.0022 | 0 | 0.0006 | 0 | 0.005 | 0.0005 |
Nucleus | 0.0064 | 0.0015 | 0.0026 | 0.0028 | 0.0012 | 0.0031 | 0.006 | 0.0009 | 0.0029 | 0.0023 | 0.001 | 0.0019 | 0.0045 | 0.0006 | 0.0035 | 0.0015 | 0.0045 | 0.0033 |
Peroxisomes | 0 | 0 | 0.0001 | 0.0068 | 0 | 0.0001 | 0.0004 | 0 | 0.0013 | 0.0016 | 0.0014 | 0.0001 | 0.0004 | 0 | 0.0004 | 0 | 0.003 | 0.0063 |
Punctate Nuclear | 0.0005 | 0 | 0.0001 | 0.0016 | 0 | 0.0061 | 0.0015 | 0 | 0.0014 | 0.0018 | 0.0003 | 0.0001 | 0.0014 | 0 | 0.004 | 0 | 0.0189 | 0.0003 |
Vacuole | 0.0877 | 0.0333 | 0.0638 | 0.1135 | 0.038 | 0.0263 | 0.0602 | 0.0176 | 0.0464 | 0.073 | 0.0205 | 0.0345 | 0.0534 | 0.0101 | 0.0388 | 0.0703 | 0.0379 | 0.0229 |
Vacuole Periphery | 0.0148 | 0.0028 | 0.011 | 0.017 | 0.0061 | 0.009 | 0.0137 | 0.0018 | 0.0128 | 0.0202 | 0.0105 | 0.0172 | 0.0172 | 0.0016 | 0.0083 | 0.0321 | 0.0209 | 0.0136 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2274.3168 | 2251.6013 | 2294.6275 | 2578.4556 | 2734.4611 | 1810.0705 | 2071.446 | 2130.147 | 2091.1497 | 2074.9688 |
Translational Efficiency | 2.0803 | 2.0747 | 2.2264 | 1.5774 | 2.0644 | 2.9046 | 2.0391 | 1.849 | 2.1644 | 2.4947 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|