Standard name
Human Ortholog
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.6 0.56 0.67 0.61 0.44 0.37 0.53 0.45 0.49 0.5 0.57 0.56 0.62 0.66 0.57 0.57 0.57 0.59 0.84 0.81 0.81 0.92 0.89 0.82
Bud 0.05 0.12 0.11 0.09 0.2 0.13 0.16 0.12 0.1 0.13 0.13 0.13 0.05 0.05 0.07 0.14 0.15 0.17 0 0.06 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.08 0.09 0.26 0.13 0 0 0.05 0 0 0 0 0.06 0.23 0.28 0.32 0.1 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.12 0.1 0 0.05 0.06 0 0 0.05 0.06 0.05 0.06 0.09 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0.12 0.27 0.07 0.13 0 0.08 0.06 0.06 0.07 0.06 0.1 0 0.22 0.14 0.13 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.35 0.17 0.64 0.47 0.54 0.17 0.7 0.6 0.52 0.54 0.45 0.47 0.42 0.46 0.48 0.44 0.46 0.42 0 0 0 0 0 0.05
Mitochondria 0.23 0.08 0 0.11 0 0.57 0.08 0.19 0.45 0.33 0.43 0.39 0 0.05 0.05 0.13 0.14 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0.08 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 47 71 51 67 64 50 119 124 87 123 118 108 119 205 178 264 185 328 101 187 101 228 293 298
Bud 4 15 8 10 29 18 36 32 18 32 26 25 10 16 23 67 50 92 4 14 6 2 6 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3 2 1 0 1
Bud Site 0 1 0 0 0 6 1 2 2 4 1 5 0 0 0 19 7 22
Cell Periphery 6 11 20 14 4 2 11 12 2 7 9 12 45 88 101 45 33 41 0 1 2 3 1 1
Cytoplasm 3 7 3 6 18 13 8 14 11 10 7 10 12 15 18 40 18 37 0 0 1 0 0 0
Endoplasmic Reticulum 9 34 5 14 6 11 13 17 13 14 20 6 43 45 41 10 11 19 0 4 1 1 2 2
Endosome 2 1 1 0 0 4 1 1 1 2 2 2 0 0 1 16 12 21 2 5 1 1 1 7
Golgi 27 22 49 52 78 23 157 164 92 135 93 91 82 143 150 206 148 230 2 6 3 3 8 19
Mitochondria 18 10 1 12 4 78 17 53 79 82 88 74 7 15 14 62 46 65 1 3 1 1 3 5
Nucleus 0 0 0 0 0 3 0 2 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 1 0 0 1 2 1 0 0 1 0 1 1 0 2 0 0 0 1 0
Nucleolus 5 2 0 1 5 11 5 8 8 9 5 6 0 0 2 4 0 7 0 0 0 0 0 1
Peroxisomes 0 0 2 4 0 4 1 6 2 6 6 8 0 6 7 19 20 35 2 0 1 1 7 11
SpindlePole 0 0 0 0 6 4 4 8 0 7 2 3 0 1 0 12 6 13 0 0 0 0 0 3
Vac/Vac Membrane 3 4 1 2 3 5 2 5 1 3 2 2 0 0 0 26 18 13 0 1 0 0 0 0
Unique Cell Count 78 127 76 110 145 136 223 274 176 248 206 192 193 312 311 465 324 552 121 231 125 248 331 365
Labelled Cell Count 124 179 141 183 217 233 375 448 318 436 380 352 318 535 535 791 557 924 121 231 125 248 331 365


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.0 28.5 25.2 22.7 24.4 15.4 22.3 21.5 19.6 20.0 19.8 19.7 27.8 29.1 30.1 12.7 12.4 12.5 25.1 26.4 28.4
Std Deviation (1e-4) 7.5 8.2 6.9 6.0 6.6 5.5 5.7 6.4 5.7 6.2 6.5 6.3 5.8 7.3 9.0 4.3 4.8 3.2 6.4 6.8 7.0
Intensity Change (Log2) -0.16 -0.05 -0.71 -0.18 -0.23 -0.36 -0.34 -0.35 -0.36 0.14 0.21 0.26 -0.99 -1.03 -1.01 -0.01 0.07 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -3.2 -4.2 -2.1 -3.4 -2.6 -2.7 -1.5 -1.6 -0.8 -0.2 -1.6 -1.7 -1.6 -1.3
Bud -0.3 1.8 0.6 1.2 0.3 -0.1 0.6 0.5 0.6 -1.6 0 -0.9 0.9 1.1 1.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.4 -5.3 -5.7 -5.3 -5.9 -6.5 -6.5 -5.4 -4.6 -0.5 0.3 1.0 -4.1 -3.7 -5.2
Cytoplasm 0 2.0 1.5 0 0 0 0 0 0 0 0 0 1.4 0 0
Endoplasmic Reticulum 1.4 0 0.4 0 0 0.2 0 0.8 0 3.0 1.8 1.6 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.3 -1.5 -7.0 1.0 -0.7 -1.8 -1.5 -2.9 -2.5 -3.2 -2.9 -2.5 -3.3 -2.9 -3.8
Mitochondria 2.5 0 8.1 0 3.8 6.8 5.5 6.6 6.1 0 0 0 3.0 3.1 2.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.8727 15.7594 14.3598 10.9379 12.0376 15.3392 22.0249 20.0899 20.5445 20.0019 17.524 21.0116 5.8932 23.8469 24.6855 24.5627 14.7282 19.8348
Actin 0.6911 0.9382 0.8846 0.7751 0.7384 0.8835 0.7533 0.9153 0.9453 0.9926 0.8708 0.8961 0.2076 0.8312 0.9806 0.9623 0.4942 0.8359
Bud 0.002 0.0005 0.0011 0.0007 0.0019 0.0007 0.0022 0.0009 0.0013 0.0001 0.0028 0.0004 0.0006 0.0004 0.0004 0.0005 0.0009 0.0117
Bud Neck 0.0079 0.002 0.0033 0.0042 0.0007 0.012 0.0015 0.0027 0.0117 0.0035 0.0011 0.0045 0.0004 0.0073 0.0072 0.0159 0.001 0.0022
Bud Periphery 0.003 0.0004 0.0013 0.0011 0.002 0.0015 0.0027 0.0012 0.0018 0.0001 0.0037 0.0004 0.0007 0.0003 0.0005 0.0007 0.0012 0.0123
Bud Site 0.0236 0.0187 0.0088 0.0015 0.002 0.0016 0.0036 0.0421 0.0106 0.0001 0.0052 0.0007 0.0019 0.1003 0.0021 0.0009 0.0045 0.0065
Cell Periphery 0.0012 0.0005 0.0004 0.0003 0.0002 0.0005 0.0005 0.001 0.0005 0 0.0003 0.0002 0.0004 0.0011 0.0002 0.0003 0.0007 0.0006
Cytoplasm 0.0115 0.0015 0.0121 0.0026 0.002 0.0008 0.0028 0.0013 0.0054 0 0.001 0.0029 0.1143 0.0002 0 0 0.0508 0.008
Cytoplasmic Foci 0.0221 0.0016 0.0103 0.0361 0.0069 0.0133 0.0066 0.001 0.0006 0.0001 0.0038 0.0029 0.0289 0.0105 0.0002 0.0001 0.0064 0.0223
Eisosomes 0.025 0.0214 0.0043 0.0021 0.0181 0.0171 0.0102 0.0124 0.0035 0.0014 0.001 0.0103 0.0017 0.0153 0.0039 0.017 0.0003 0.0014
Endoplasmic Reticulum 0.0057 0.0001 0.0025 0.012 0.0014 0.0005 0.0031 0.0002 0.0004 0 0.0012 0.0006 0.0309 0 0 0 0.0096 0.0019
Endosome 0.0555 0.0009 0.0218 0.0461 0.0238 0.0123 0.0202 0.0004 0.001 0 0.0507 0.0173 0.1747 0.0002 0.0001 0 0.0993 0.0219
Golgi 0.0379 0.0056 0.0151 0.0645 0.0536 0.0143 0.0541 0.0057 0.0032 0.0017 0.0451 0.0231 0.0092 0.0038 0.0043 0.0019 0.0064 0.033
Lipid Particles 0.0134 0.0003 0.0039 0.0278 0.0043 0.019 0.0141 0.0006 0.0004 0 0.0007 0.0004 0.0187 0.0005 0.0001 0.0001 0.0048 0.0062
Mitochondria 0.0368 0.0015 0.0147 0.008 0.0726 0.0089 0.055 0.0039 0.0012 0 0.0086 0.0007 0.0095 0.0004 0.0001 0.0001 0.0046 0.0103
None 0.0121 0.0034 0.0015 0.0026 0.0301 0.0022 0.0019 0.0085 0.0006 0 0.0001 0.0002 0.0911 0 0 0 0.0355 0.0006
Nuclear Periphery 0.0146 0.0001 0.0035 0.0047 0.016 0.0011 0.0095 0.0004 0.002 0 0.0005 0.0135 0.1527 0 0 0 0.0506 0.0011
Nucleolus 0.0016 0.0011 0.0001 0.0001 0.0041 0.0006 0.0039 0.0005 0.0001 0 0 0.0016 0.004 0 0 0 0.0146 0.0002
Nucleus 0.0076 0.0004 0.0006 0.0003 0.0053 0.0005 0.0426 0.0005 0.0092 0 0.0001 0.0163 0.0422 0 0 0 0.1282 0.0012
Peroxisomes 0.0077 0.0007 0.0027 0.0072 0.0038 0.0057 0.0032 0.0007 0.0004 0.0001 0.0006 0.0002 0.002 0.0282 0.0001 0.0001 0.0008 0.0022
Punctate Nuclear 0.0047 0.0009 0.0009 0.0015 0.009 0.0023 0.0029 0.0003 0.0005 0 0.0001 0.0019 0.0823 0.0001 0 0 0.0105 0.0007
Vacuole 0.0092 0.0001 0.0036 0.0006 0.0017 0.0008 0.0028 0.0002 0.0001 0 0.001 0.004 0.0143 0 0 0 0.0636 0.016
Vacuole Periphery 0.0058 0.0001 0.0027 0.0009 0.0021 0.0006 0.0033 0.0001 0.0001 0 0.0014 0.0017 0.0119 0 0 0 0.0114 0.0037

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.8891 38.5734 51.7942 64.2414 47.9535 55.4477 59.4308 57.6607 54.9624 72.7546
Translational Efficiency 1.7317 2.3784 2.0768 1.3713 2.2491 2.9978 2.01 2.6916 3.0507 2.1866

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
Localization
Cell Percentages actin (96%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Arc18

Arc18


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arc18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available