Standard name
Description Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.6 0.56 0.67 0.61 0.44 0.37 0.53 0.45 0.49 0.5 0.57 0.56 0.62 0.66 0.57 0.57 0.57 0.59 0.84 0.81 0.81 0.92 0.89 0.82
Bud 0.05 0.12 0.11 0.09 0.2 0.13 0.16 0.12 0.1 0.13 0.13 0.13 0.05 0.05 0.07 0.14 0.15 0.17 0 0.06 0.05 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.08 0.09 0.26 0.13 0 0 0.05 0 0 0 0 0.06 0.23 0.28 0.32 0.1 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0 0.06 0 0.05 0.12 0.1 0 0.05 0.06 0 0 0.05 0.06 0.05 0.06 0.09 0.06 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0.12 0.27 0.07 0.13 0 0.08 0.06 0.06 0.07 0.06 0.1 0 0.22 0.14 0.13 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.35 0.17 0.64 0.47 0.54 0.17 0.7 0.6 0.52 0.54 0.45 0.47 0.42 0.46 0.48 0.44 0.46 0.42 0 0 0 0 0 0.05
Mitochondria 0.23 0.08 0 0.11 0 0.57 0.08 0.19 0.45 0.33 0.43 0.39 0 0.05 0.05 0.13 0.14 0.12 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0 0 0.08 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 47 71 51 67 64 50 119 124 87 123 118 108 119 205 178 264 185 328 101 187 101 228 293 298
Bud 4 15 8 10 29 18 36 32 18 32 26 25 10 16 23 67 50 92 4 14 6 2 6 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 3 2 1 0 1
Bud Site 0 1 0 0 0 6 1 2 2 4 1 5 0 0 0 19 7 22
Cell Periphery 6 11 20 14 4 2 11 12 2 7 9 12 45 88 101 45 33 41 0 1 2 3 1 1
Cytoplasm 3 7 3 6 18 13 8 14 11 10 7 10 12 15 18 40 18 37 0 0 1 0 0 0
Endoplasmic Reticulum 9 34 5 14 6 11 13 17 13 14 20 6 43 45 41 10 11 19 0 4 1 1 2 2
Endosome 2 1 1 0 0 4 1 1 1 2 2 2 0 0 1 16 12 21 2 5 1 1 1 7
Golgi 27 22 49 52 78 23 157 164 92 135 93 91 82 143 150 206 148 230 2 6 3 3 8 19
Mitochondria 18 10 1 12 4 78 17 53 79 82 88 74 7 15 14 62 46 65 1 3 1 1 3 5
Nucleus 0 0 0 0 0 3 0 2 1 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 1 0 0 1 2 1 0 0 1 0 1 1 0 2 0 0 0 1 0
Nucleolus 5 2 0 1 5 11 5 8 8 9 5 6 0 0 2 4 0 7 0 0 0 0 0 1
Peroxisomes 0 0 2 4 0 4 1 6 2 6 6 8 0 6 7 19 20 35 2 0 1 1 7 11
SpindlePole 0 0 0 0 6 4 4 8 0 7 2 3 0 1 0 12 6 13 0 0 0 0 0 3
Vac/Vac Membrane 3 4 1 2 3 5 2 5 1 3 2 2 0 0 0 26 18 13 0 1 0 0 0 0
Unique Cell Count 78 127 76 110 145 136 223 274 176 248 206 192 193 312 311 465 324 552 121 231 125 248 331 365
Labelled Cell Count 124 179 141 183 217 233 375 448 318 436 380 352 318 535 535 791 557 924 121 231 125 248 331 365


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.0 28.5 25.2 22.7 24.4 15.4 22.3 21.5 19.6 20.0 19.8 19.7 27.8 29.1 30.1 12.7 12.4 12.5 25.1 26.4 28.4
Std Deviation (1e-4) 7.5 8.2 6.9 6.0 6.6 5.5 5.7 6.4 5.7 6.2 6.5 6.3 5.8 7.3 9.0 4.3 4.8 3.2 6.4 6.8 7.0
Intensity Change (Log2) -0.16 -0.05 -0.71 -0.18 -0.23 -0.36 -0.34 -0.35 -0.36 0.14 0.21 0.26 -0.99 -1.03 -1.01 -0.01 0.07 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.9 -3.2 -4.2 -2.1 -3.4 -2.6 -2.7 -1.5 -1.6 -0.8 -0.2 -1.6 -1.7 -1.6 -1.3
Bud -0.3 1.8 0.6 1.2 0.3 -0.1 0.6 0.5 0.6 -1.6 0 -0.9 0.9 1.1 1.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -2.4 -5.3 -5.7 -5.3 -5.9 -6.5 -6.5 -5.4 -4.6 -0.5 0.3 1.0 -4.1 -3.7 -5.2
Cytoplasm 0 2.0 1.5 0 0 0 0 0 0 0 0 0 1.4 0 0
Endoplasmic Reticulum 1.4 0 0.4 0 0 0.2 0 0.8 0 3.0 1.8 1.6 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -2.3 -1.5 -7.0 1.0 -0.7 -1.8 -1.5 -2.9 -2.5 -3.2 -2.9 -2.5 -3.3 -2.9 -3.8
Mitochondria 2.5 0 8.1 0 3.8 6.8 5.5 6.6 6.1 0 0 0 3.0 3.1 2.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Arc18-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available