Standard name
Human Ortholog
Description Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex allows SRP-independent protein import into ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.09 0.22 0.13 0.11 0.14 0.09 0.17 0.18 0.2 0.07 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 0.07 0.13 0.08 0.08 0.12 0.06 0.15 0.12 0.15 0.55 0.26 0.42 0 0 0 0 0.18 0
Cytoplasm 0.18 0.07 0.06 0.09 0.13 0.13 0.2 0.22 0.22 0.21 0.2 0.24 0.19 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.74 0.84 0.72 0.75 0.8 0.73 0.68 0.6 0.58 0.63 0 0.07 0 0 0.8 0.88 0.99 0.52 0.75
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.14 0.24 0.18 0.05 0 0 0 0 0.07 0.17 0.43 0.38 0 0 0 0 0.09 0
Mitochondria 0 0 0.06 0 0 0 0 0 0.07 0.05 0.12 0.16 0 0 0 0 0 0.07 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 23 41 25 26 37 23 32 39 39 8 4 3 0 3 9 0 0 0
Bud 1 4 2 0 2 4 5 2 4 3 0 3 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 12 17 24 16 18 32 16 28 26 29 63 32 31 0 4 7 0 2 1
Cytoplasm 23 17 11 18 30 36 55 41 47 40 23 29 14 0 11 1 0 0 0
Endoplasmic Reticulum 93 210 136 144 187 197 182 113 125 122 3 9 3 0 142 236 2 8 18
Endosome 0 0 0 0 1 0 1 0 1 0 0 0 0 0 0 1 0 0 0
Golgi 5 35 45 35 12 12 9 8 5 13 20 52 28 0 1 2 0 1 0
Mitochondria 1 5 12 1 5 3 2 3 16 9 14 19 3 0 6 7 0 1 0
Nucleus 0 0 0 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 2 1 0 3 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 2 1 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 3 2 3 6 7 9 1 6 3 3 5 3 0 3 0 0 0 0
Unique Cell Count 126 251 189 192 234 271 269 188 217 194 115 122 73 1 178 270 3 16 25
Labelled Cell Count 141 314 273 242 289 328 304 230 271 263 134 154 85 1 178 270 3 16 25


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 58.7 32.3 33.3 32.3 28.6 26.9 25.2 25.4 22.9 24.3 25.1 29.5 35.3
Std Deviation (1e-4) 17.1 8.6 8.4 7.6 6.7 5.3 6.0 7.3 6.8 6.4 7.5 10.3 12.1
Intensity Change (Log2) 0.04 0.0 -0.18 -0.26 -0.36 -0.35 -0.49 -0.41 -0.36 -0.13 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.7 1.3 0.7 1.6 -0.2 2.5 2.8 3.3 -0.7 -2.1 -1.4
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.1 0.6 0.4 2.0 -0.4 2.8 1.9 2.8 10.3 5.2 7.6
Cytoplasm -0.4 1.0 2.2 2.5 4.5 4.6 4.7 4.3 3.8 4.7 3.2
Endoplasmic Reticulum -3.0 -2.3 -1.1 -3.0 -4.2 -5.5 -6.2 -5.0 -14.6 -14.0 -12.6
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 2.7 1.2 -3.3 -3.8 -4.3 -3.4 -4.5 -2.4 0.9 6.1 4.6
Mitochondria 2.3 0 0 0 0 0 2.8 1.6 4.1 5.0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 41.9299 45.1613 43.8676 40.9945 38.2308 45.2422 37.8505 36.0782 35.7977 37.3664 33.2346 42.1665 46.9603 47.141 49.6898 50.4847 43.6263 52.0283
Actin 0.0015 0.001 0.0012 0.0005 0.0007 0.0006 0.0338 0.0221 0.0296 0 0.0781 0.0017 0.0374 0.0039 0.0047 0.0004 0.007 0.0006
Bud 0.0003 0.0002 0.0005 0.0001 0.0019 0.0001 0.0016 0.0007 0.001 0 0.0011 0.0001 0.0003 0.0001 0.0002 0.0001 0.0015 0
Bud Neck 0.0004 0.0005 0.0005 0.002 0.0008 0.0023 0.0102 0.004 0.0012 0.0002 0.0031 0.0017 0.0008 0.0006 0.0004 0.0001 0.0009 0.0009
Bud Periphery 0.0014 0.0004 0.0008 0.0002 0.0012 0.0003 0.0103 0.0018 0.0012 0.0001 0.0016 0.0007 0.0013 0.0006 0.0003 0.0001 0.0031 0.0002
Bud Site 0.0003 0.0008 0.001 0.0004 0.006 0.0003 0.0039 0.0089 0.0047 0 0.0026 0 0.0009 0.0008 0.0007 0.0002 0.0018 0
Cell Periphery 0.024 0.057 0.0266 0.0225 0.0063 0.0155 0.0242 0.0695 0.0428 0.0227 0.0307 0.0077 0.0136 0.0208 0.0207 0.0057 0.0129 0.0053
Cytoplasm 0.0227 0.0343 0.0135 0.0103 0.074 0.0208 0.0132 0.014 0.032 0.0006 0.026 0.0127 0.007 0.0457 0.0298 0.0027 0.0018 0.0018
Cytoplasmic Foci 0.0128 0.0039 0.0034 0.003 0.0061 0.0003 0.0304 0.0018 0.0083 0 0.0172 0.0005 0.0067 0.0088 0.0055 0.0047 0.0064 0.0004
Eisosomes 0.0008 0.0014 0.0018 0.0062 0.0001 0.0006 0.0037 0.0111 0.0013 0.0002 0.0032 0.0005 0.0015 0.0002 0.0011 0.0003 0.0008 0.0002
Endoplasmic Reticulum 0.757 0.8131 0.8852 0.8162 0.7895 0.8838 0.5979 0.7962 0.7424 0.9601 0.5637 0.8627 0.7235 0.833 0.8494 0.8596 0.8284 0.9222
Endosome 0.0121 0.0007 0.0025 0.0302 0.0173 0.0025 0.0159 0.0027 0.0079 0.0002 0.0143 0.0033 0.0069 0.0062 0.0087 0.0017 0.0209 0.0015
Golgi 0.0016 0.0028 0.0053 0.003 0.0041 0.0014 0.0059 0.0053 0.0053 0.0005 0.0266 0.0038 0.0065 0.008 0.0051 0.0009 0.0109 0.0019
Lipid Particles 0.0297 0.0294 0.012 0.0059 0.033 0.0141 0.025 0.0089 0.028 0.0009 0.0321 0.0014 0.0143 0.0202 0.0196 0.0125 0.0138 0.0037
Mitochondria 0.0042 0.0004 0.0057 0.0039 0.0007 0.0127 0.0031 0.0146 0.0034 0.0001 0.09 0.0059 0.008 0.0004 0.0089 0.0057 0.0209 0.0016
None 0.0043 0.0002 0.002 0.0092 0.007 0.0002 0.0042 0.0034 0.0067 0 0.0436 0.0006 0.0088 0.0004 0.0043 0.0042 0.0016 0.0004
Nuclear Periphery 0.0849 0.0279 0.0239 0.0666 0.027 0.0306 0.0986 0.0256 0.0434 0.0106 0.0376 0.0836 0.0987 0.0334 0.0261 0.0575 0.0239 0.0502
Nucleolus 0.0011 0.0044 0.0007 0.0035 0.0001 0.0008 0.0033 0.0005 0.003 0 0.0004 0.0001 0.011 0 0.001 0.0024 0.004 0.0004
Nucleus 0.0079 0.0018 0.0024 0.0032 0.0034 0.0057 0.0088 0.0008 0.0044 0.0001 0.0059 0.0028 0.0088 0.0027 0.0011 0.0034 0.0046 0.0007
Peroxisomes 0.0042 0.0004 0.0005 0.0002 0.0002 0.0001 0.004 0.0005 0.0012 0 0.0101 0 0.0019 0.0038 0.0011 0.0005 0.0026 0
Punctate Nuclear 0.0026 0.0003 0.0003 0.0009 0.001 0.0004 0.082 0.0002 0.0093 0 0.003 0.0006 0.0285 0.0006 0.0012 0.0003 0.0024 0.0001
Vacuole 0.021 0.0176 0.0086 0.0073 0.0165 0.0055 0.0105 0.0057 0.0188 0.0032 0.0069 0.0051 0.007 0.0083 0.0078 0.0099 0.0185 0.004
Vacuole Periphery 0.0052 0.0016 0.0019 0.0046 0.0032 0.0015 0.0094 0.0017 0.004 0.0005 0.0023 0.0044 0.0064 0.0013 0.0021 0.027 0.0115 0.0038

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 295.9234 282.31 240.4273 238.1758 258.2637 282.7676 314.2987 253.2971 266.386 287.3364
Translational Efficiency 0.8761 0.9549 0.9651 1.0019 1.0058 0.9729 0.8626 0.9462 0.9161 0.8397

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency