Standard name
Description Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma; Small Subunit processome is also known as SSU processome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 – – 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0.07 0.06 0.09 0.06 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cytoplasm – – 0 0 0 0 0 0 0 0 0 0 0.08 0.07 0.1 0 0 0 – – 0 0 0 0
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Mitochondria – – 0.28 0.22 0.12 0.27 0.25 0.2 0.34 0.29 0.28 0.24 0 0 0.1 0.06 0 0.07 – – 0 0 0 0
Nucleus – – 0.29 0.16 0.07 0.1 0.08 0.07 0 0.05 0.11 0.09 0.13 0.06 0.07 0.14 0.16 0.16 – – 0.17 0.14 0.13 0.06
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Nucleolus – – 0.83 0.88 0.93 0.87 0.84 0.86 0.82 0.87 0.81 0.85 0.86 0.89 0.84 0.88 0.86 0.87 – – 0.68 0.73 0.71 0.76
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
SpindlePole – – 0.06 0.14 0.2 0.2 0.3 0.32 0.25 0.31 0.3 0.35 0.08 0.06 0 0.05 0.05 0 – – 0.08 0 0.07 0.09
Vac/Vac Membrane – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Bud – – 0 1 2 3 0 13 3 4 6 2 0 0 1 0 0 0 – – 2 0 4 1
Bud Neck – – 1 5 4 3 6 9 3 8 2 4 1 7 3 5 1 3 – – 0 0 0 2
Bud Site – – 1 5 7 8 13 17 12 20 16 18 0 0 1 0 0 0 – – – – – –
Cell Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 0
Cytoplasm – – 1 7 7 3 9 7 5 6 7 6 11 10 21 15 8 15 – – 0 0 0 0
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 2 – – 0 0 0 0
Endosome – – 0 0 9 1 7 7 2 8 6 3 0 6 3 2 10 3 – – 1 2 3 3
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – 0 0 0 1
Mitochondria – – 35 44 40 56 80 91 60 96 49 66 5 5 21 24 6 24 – – 0 0 0 1
Nucleus – – 36 32 21 22 25 32 5 18 20 24 18 9 13 56 41 59 – – 21 11 23 11
Nuclear Periphery – – 1 0 0 2 0 3 0 2 2 2 0 0 0 0 0 0 – – 0 0 0 0
Nucleolus – – 104 178 299 183 270 392 147 293 143 238 118 127 169 362 220 316 – – 84 58 129 141
Peroxisomes – – 0 0 1 1 2 2 4 1 0 1 1 1 2 4 1 0 – – 0 0 0 0
SpindlePole – – 8 28 65 43 95 145 45 105 53 99 11 9 9 21 12 12 – – 9 1 13 17
Vac/Vac Membrane – – 1 0 1 0 0 1 0 0 0 0 1 0 0 2 1 3 – – 2 2 4 2
Unique Cell Count 125 203 321 210 320 457 179 336 176 280 137 143 200 411 255 364 123 81 182 187
Labelled Cell Count 188 300 456 325 507 719 286 561 304 463 166 174 243 494 301 437 123 81 182 187


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 7.4 8.5 8.5 7.0 6.3 5.8 5.5 5.6 5.2 5.0 11.7 11.8 10.1 17.8 20.3 19.5 11.4 12.7 14.5
Std Deviation (1e-4) – – 1.6 1.8 1.8 1.8 1.6 1.2 1.4 1.5 1.5 1.3 3.1 2.9 3.2 5.4 6.9 5.7 2.3 3.5 4.2
Intensity Change (Log2) – – – 0.19 0.19 -0.08 -0.25 -0.37 -0.43 -0.4 -0.52 -0.57 0.66 0.67 0.45 1.26 1.45 1.39 0.62 0.77 0.97


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 2.5 2.4 3.1 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.8 2.5 3.4 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.3 -3.9 -0.3 -0.6 -1.9 1.0 0.1 0 -1.0 -5.5 -5.6 -4.1 -6.9 -7.6 -6.3
Nucleus -2.8 -6.3 -4.3 -5.8 -6.7 -6.5 -7.0 -3.8 -5.3 -3.1 -4.9 -5.5 -3.9 -2.9 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.1 3.2 1.0 0.3 0.7 -0.2 1.1 -0.4 0.5 0.7 1.3 0.3 1.4 0.8 1.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.1 3.6 3.5 5.2 5.7 4.2 5.5 5.0 6.1 0.5 0 -0.7 -0.6 -0.7 -1.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.1516 13.3038 11.758 12.0138 8.8549 11.7987 11.7993 16.4916 14.1575 13.0621 13.6043 14.421 10.3475 12.0916 11.2931 10.3589 10.6856 10.6977
Actin 0.0019 0 0.0053 0 0.0003 0 0.0035 0 0.0007 0.0041 0.0012 0 0.0117 0 0.0024 0 0 0
Bud 0.0001 0.0001 0.0003 0 0.0003 0.0001 0.0002 0.0001 0.0002 0.0005 0.0003 0 0.0006 0.0043 0.0001 0.0001 0.0001 0.0002
Bud Neck 0.0002 0.0011 0.0005 0.0006 0.002 0.0021 0.0004 0.0072 0.0005 0.0012 0.0006 0.0004 0.0006 0.0014 0.0004 0.001 0.0008 0.0004
Bud Periphery 0.0001 0.0001 0.0004 0.0001 0.0004 0.0002 0.0003 0.0003 0.0003 0.0006 0.0006 0 0.0011 0.0022 0.0001 0.0001 0.0001 0.0001
Bud Site 0.0003 0.0017 0.0017 0.0004 0.001 0.0002 0.0014 0.0008 0.0055 0.002 0.0005 0.0001 0.001 0.0098 0.0003 0.0005 0.0003 0.0003
Cell Periphery 0.0002 0.0001 0.0005 0.0003 0.0006 0.001 0.0002 0.0003 0.0004 0.0006 0.0002 0.0001 0.0008 0.0006 0.0001 0.0004 0.0002 0.0001
Cytoplasm 0.0024 0 0.0021 0 0.0003 0 0.0006 0 0.0007 0.0006 0.001 0 0.0002 0.0004 0.0002 0 0 0
Cytoplasmic Foci 0.0055 0.0001 0.0019 0.0003 0.0356 0.0004 0.006 0.0001 0.0025 0.0083 0.0013 0.0001 0.0057 0.0126 0.003 0.0011 0.0035 0.0001
Eisosomes 0 0 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0012 0 0.0014 0 0.0001 0 0.0005 0 0.0005 0.0011 0.0033 0 0.0002 0 0 0 0 0
Endosome 0.0057 0 0.0074 0 0.0097 0.0002 0.0109 0.0001 0.0091 0.0168 0.0287 0 0.0026 0.0001 0.0021 0.0001 0.0002 0
Golgi 0.0035 0 0.0005 0 0.0052 0.0001 0.0035 0 0.0015 0.0096 0.0028 0 0.0025 0.0001 0.0054 0 0.0003 0
Lipid Particles 0.007 0.0001 0.0031 0.0006 0.0457 0.002 0.0107 0.0002 0.0054 0.0115 0.0008 0.0003 0.0064 0.002 0.0037 0.0019 0.0255 0.0002
Mitochondria 0.0048 0 0.0031 0.0001 0.0317 0.0022 0.0033 0.0001 0.0059 0.0109 0.0157 0.0001 0.0012 0.0003 0.0018 0.002 0.0092 0
None 0.0003 0 0.0008 0 0.0005 0 0.0006 0 0.0004 0.0002 0.0004 0 0.0003 0.0008 0.0001 0 0 0
Nuclear Periphery 0.0055 0 0.0026 0 0.0011 0 0.0075 0.0001 0.0032 0.0007 0.0081 0 0.0002 0.0004 0.0004 0.0001 0.0001 0.0001
Nucleolus 0.8582 0.8668 0.8865 0.914 0.7394 0.8863 0.8474 0.8454 0.8317 0.8243 0.8334 0.8911 0.8673 0.8018 0.8436 0.7768 0.8533 0.8709
Nucleus 0.0948 0.1287 0.072 0.0792 0.1007 0.0983 0.0906 0.1425 0.1178 0.0933 0.0926 0.1069 0.0653 0.1474 0.1222 0.2137 0.0895 0.1197
Peroxisomes 0.0015 0 0.0004 0 0.0036 0.0002 0.0015 0 0.0005 0.0021 0.0003 0 0.0027 0.0002 0.0017 0.0004 0.003 0
Punctate Nuclear 0.0051 0.0006 0.0058 0.0038 0.0112 0.0034 0.0086 0.0009 0.0066 0.0037 0.0025 0.0007 0.0249 0.0139 0.0119 0.0007 0.0133 0.0077
Vacuole 0.0009 0.0004 0.0025 0.0006 0.0061 0.0023 0.0015 0.0014 0.0046 0.0056 0.0027 0.0001 0.0037 0.0014 0.0004 0.0009 0.0004 0.0001
Vacuole Periphery 0.0007 0 0.0009 0 0.0043 0.0009 0.0009 0.0004 0.0018 0.0022 0.0031 0 0.0008 0.0002 0.0001 0.0002 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 52.2356 39.8748 33.2225 43.8708 59.1015 31.2849 38.4606 31.2693 28.0346 50.82
Translational Efficiency 0.9782 1.0081 1.1822 0.6232 0.5726 1.126 0.9355 0.6323 0.7355 0.6995

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘