| ORF | |
|---|---|
| Description | Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 0 | 2 | 2 |
| Bud | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 6 | 6 | 6 | 8 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 2 | 4 |
| Bud Neck | 0 | 0 | 7 | 4 | 14 | 6 | 8 | 19 | 8 | 15 | 3 | 11 | 5 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 2 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 2 | 0 | 5 | 3 | 0 | 5 | 1 | 4 | 5 | 1 | 0 | 1 | 1 | 0 | 1 | 4 | 2 | 1 | 0 | 0 | 1 | 1 | 3 | 2 |
| Cytoplasm | 155 | 169 | 179 | 335 | 407 | 387 | 549 | 568 | 403 | 432 | 219 | 289 | 237 | 202 | 294 | 97 | 91 | 62 | 113 | 150 | 144 | 96 | 246 | 275 |
| Endoplasmic Reticulum | 1 | 1 | 2 | 1 | 2 | 2 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 7 | 6 | 4 | 3 | 0 | 0 | 0 | 0 | 1 |
| Endosome | 0 | 0 | 0 | 0 | 3 | 3 | 0 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 2 |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 3 | 0 | 0 | 1 | 1 |
| Mitochondria | 25 | 3 | 0 | 3 | 6 | 22 | 72 | 86 | 132 | 95 | 135 | 87 | 0 | 0 | 1 | 2 | 2 | 3 | 16 | 10 | 17 | 3 | 11 | 10 |
| Nucleus | 0 | 0 | 0 | 2 | 2 | 3 | 2 | 1 | 3 | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| Nuclear Periphery | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 0 | 2 | 3 | 4 | 10 | 12 | 2 | 6 | 3 | 6 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 5 |
| Vac/Vac Membrane | 1 | 0 | 0 | 2 | 6 | 6 | 13 | 11 | 2 | 6 | 4 | 6 | 1 | 0 | 0 | 1 | 5 | 0 | 3 | 4 | 1 | 1 | 0 | 5 |
| Unique Cell Count | 164 | 172 | 182 | 341 | 420 | 405 | 603 | 637 | 476 | 508 | 306 | 353 | 238 | 202 | 295 | 100 | 97 | 65 | 163 | 178 | 181 | 110 | 277 | 322 |
| Labelled Cell Count | 186 | 174 | 194 | 355 | 443 | 438 | 662 | 719 | 567 | 568 | 374 | 408 | 244 | 202 | 297 | 113 | 108 | 71 | 163 | 178 | 181 | 110 | 277 | 322 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.3 | 4.3 | 4.6 | 4.4 | 4.6 | 4.9 | 4.1 | 4.1 | 3.3 | 3.5 | 2.9 | 3.4 | 5.0 | 5.0 | 4.8 | 5.3 | 6.4 | 5.7 | 4.4 | 4.6 | 5.1 |
| Std Deviation (1e-4) | 1.2 | 1.2 | 1.2 | 1.7 | 1.6 | 2.7 | 1.9 | 1.6 | 1.0 | 1.3 | 0.8 | 1.0 | 1.5 | 1.2 | 1.5 | 1.7 | 2.8 | 2.9 | 1.8 | 1.1 | 1.9 |
| Intensity Change (Log2) | – | – | – | -0.08 | 0.0 | 0.07 | -0.15 | -0.19 | -0.5 | -0.38 | -0.65 | -0.42 | 0.11 | 0.11 | 0.06 | 0.2 | 0.48 | 0.31 | -0.07 | -0.01 | 0.14 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Yml131w-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
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Images are not yet available
Images are not yet available
