ORF
Description Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.98 0.98 0.97 0.96 0.91 0.89 0.85 0.85 0.72 0.82 1.0 1.0 1.0 0.97 0.94 0.95 0.69 0.85 0.8 0.88 0.89 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0.05 0.12 0.14 0.28 0.19 0.44 0.25 0 0 0 0 0 0.05 0.1 0.06 0.1 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 3 1 3 0 2 2
Bud 0 0 1 2 0 0 3 6 6 6 8 5 0 0 0 0 0 0 1 2 4 0 2 4
Bud Neck 0 0 7 4 14 6 8 19 8 15 3 11 5 0 0 1 1 0 0 0 1 0 0 2
Bud Site 0 0 0 0 0 0 0 1 2 0 1 1 0 0 0 0 0 0
Cell Periphery 2 0 5 3 0 5 1 4 5 1 0 1 1 0 1 4 2 1 0 0 1 1 3 2
Cytoplasm 155 169 179 335 407 387 549 568 403 432 219 289 237 202 294 97 91 62 113 150 144 96 246 275
Endoplasmic Reticulum 1 1 2 1 2 2 2 2 1 0 1 0 0 0 0 7 6 4 3 0 0 0 0 1
Endosome 0 0 0 0 3 3 0 4 0 0 0 1 0 0 0 0 0 1 2 1 1 0 0 2
Golgi 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 1 0 3 3 0 0 1 1
Mitochondria 25 3 0 3 6 22 72 86 132 95 135 87 0 0 1 2 2 3 16 10 17 3 11 10
Nucleus 0 0 0 2 2 3 2 1 3 4 0 1 0 0 0 1 0 0 0 0 0 0 0 1
Nuclear Periphery 2 1 0 1 0 0 0 2 2 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 2 3 4 10 12 2 6 3 6 0 0 1 0 0 0 1 0 1 0 2 5
Vac/Vac Membrane 1 0 0 2 6 6 13 11 2 6 4 6 1 0 0 1 5 0 3 4 1 1 0 5
Unique Cell Count 164 172 182 341 420 405 603 637 476 508 306 353 238 202 295 100 97 65 163 178 181 110 277 322
Labelled Cell Count 186 174 194 355 443 438 662 719 567 568 374 408 244 202 297 113 108 71 163 178 181 110 277 322


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.3 4.6 4.4 4.6 4.9 4.1 4.1 3.3 3.5 2.9 3.4 5.0 5.0 4.8 5.3 6.4 5.7 4.4 4.6 5.1
Std Deviation (1e-4) 1.2 1.2 1.2 1.7 1.6 2.7 1.9 1.6 1.0 1.3 0.8 1.0 1.5 1.2 1.5 1.7 2.8 2.9 1.8 1.1 1.9
Intensity Change (Log2) -0.08 0.0 0.07 -0.15 -0.19 -0.5 -0.38 -0.65 -0.42 0.11 0.11 0.06 0.2 0.48 0.31 -0.07 -0.01 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yml131w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available