Standard name
Human Ortholog
Description Putative RNA-binding protein; associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.05 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.95 0.92 0.86 0.77 0.7 0.63 0.62 0.51 0.5 0.99 0.99 0.99 0 0 0.77 0.88 0.83 0.83
Endoplasmic Reticulum 0 0 0.08 0.05 0.07 0.06 0.05 0 0.06 0 0 0 0 0 0.11 0.06 0.11 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.09 0.29 0.33 0.36 0.47 0.59 0.58 0 0 0 0 0.8 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.05 0.05 0 0.06 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 1 3 4 2 1 3 1 3 0 0 0 2 0 3 1
Bud 5 5 2 6 10 5 6 9 9 3 3 7 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 8 4 10 10 14 15 27 17 17 29 17 42 0 0 0 0 0 0
Cytoplasm 534 244 313 278 438 418 318 170 206 643 638 650 0 0 469 399 395 455
Endoplasmic Reticulum 13 3 29 17 46 42 24 11 24 9 4 7 0 0 67 29 51 50
Endosome 1 0 2 4 1 7 2 1 1 0 0 0 0 0 3 2 2 6
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 2
Mitochondria 14 32 33 107 207 237 238 197 237 0 0 4 0 0 36 2 5 2
Nucleus 1 0 0 6 2 6 1 1 3 0 1 1 0 0 0 0 0 0
Nuclear Periphery 1 0 2 0 1 4 6 0 3 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 12 3 8 6 42 33 25 11 26 0 1 1 0 0 13 9 5 10
Unique Cell Count 562 266 362 363 629 662 510 336 412 652 647 659 1 1 607 452 477 546
Labelled Cell Count 590 294 399 435 764 771 649 418 529 685 667 712 1 1 607 452 477 546


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.9 6.3 5.8 5.7 5.8 5.2 5.2 4.9 8.3 8.5 7.6 8.7 9.3 9.8
Std Deviation (1e-4) 0.7 0.8 0.9 1.1 1.2 1.1 1.0 0.8 0.9 1.0 1.0 0.9 1.2 1.3 1.4
Intensity Change (Log2) -0.02 0.1 -0.03 -0.05 -0.03 -0.18 -0.18 -0.27 0.49 0.52 0.36 0.56 0.65 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.1 0 0.5 2.1 1.7 0 0 0.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.4 1.4 1.0 1.1 3.6 3.2 2.6 3.1 1.5 4.4
Cytoplasm -1.9 -4.6 -8.4 -11.3 -13.4 -13.2 -15.7 -16.2 3.6 3.6 3.7
Endoplasmic Reticulum -1.2 4.1 2.0 4.0 3.4 2.1 0.9 2.8 -1.2 -2.5 -1.7
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.6 4.5 11.9 13.5 14.4 17.0 19.2 19.4 -4.1 -4.0 -2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.1 -0.5 3.8 2.6 2.5 1.0 3.3 -3.7 -3.3 -3.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.7156 6.9627 6.811 5.969 6.6304 6.2397 4.0746 5.3061 4.8218 4.6793 4.7833 5.0209 8.9026 9.1922 9.3096 9.1947 9.6673 9.3477
Actin 0.008 0.0021 0.0146 0.1204 0.0009 0.001 0.0053 0.0025 0.0056 0.001 0.0015 0.0018 0.0707 0.0024 0.015 0.0035 0.0447 0.0014
Bud 0.0002 0.0022 0.0012 0.0006 0.0001 0.0002 0.0002 0.0038 0.0014 0.0003 0.0003 0.0011 0.0008 0.0003 0.0007 0.0007 0.0012 0.0008
Bud Neck 0.0005 0.0004 0.0038 0.0018 0.0004 0.0017 0.0005 0.0004 0.0009 0.0004 0.0012 0.002 0.0128 0.0003 0.0005 0.0003 0.001 0.0048
Bud Periphery 0.0003 0.0008 0.001 0.0011 0.0002 0.0003 0.0002 0.0012 0.0009 0.0002 0.0001 0.0009 0.0013 0.0001 0.0009 0.0003 0.0018 0.0005
Bud Site 0.0004 0.0102 0.0228 0.001 0.0001 0.0002 0.0005 0.006 0.0063 0.0002 0.0002 0.0049 0.0037 0.0034 0.0029 0.0002 0.0026 0.0022
Cell Periphery 0.001 0.0011 0.0021 0.0056 0.0013 0.0006 0.0004 0.0012 0.0007 0.0001 0.0001 0.0008 0.0005 0.0004 0.0007 0.0001 0.0003 0.0002
Cytoplasm 0.5988 0.8691 0.7909 0.5534 0.8719 0.7898 0.7354 0.8324 0.7666 0.7706 0.826 0.7764 0.6386 0.8821 0.7787 0.7149 0.7461 0.84
Cytoplasmic Foci 0.0062 0.0089 0.0123 0.0201 0.0038 0.0221 0.0188 0.0208 0.0144 0.0155 0.0283 0.019 0.0303 0.0207 0.0208 0.019 0.0277 0.0266
Eisosomes 0.0001 0 0.0001 0.0007 0 0 0.0003 0 0.0001 0 0 0 0.0005 0 0.0001 0 0.0006 0
Endoplasmic Reticulum 0.2064 0.0682 0.1022 0.0903 0.0957 0.0956 0.119 0.0575 0.1238 0.1362 0.0531 0.0875 0.1113 0.0454 0.084 0.1588 0.037 0.0701
Endosome 0.0484 0.0147 0.018 0.0603 0.0111 0.0364 0.0679 0.0382 0.0452 0.0439 0.0554 0.0595 0.0399 0.0259 0.0446 0.0755 0.0598 0.0375
Golgi 0.0123 0.0017 0.0046 0.0139 0.0018 0.004 0.0058 0.0079 0.0079 0.0046 0.0114 0.0052 0.0123 0.0045 0.0141 0.0078 0.0217 0.0046
Lipid Particles 0.0013 0.0004 0.0018 0.0064 0.0002 0.0005 0.0015 0.0008 0.0008 0.0001 0.0004 0.0006 0.004 0.0009 0.0055 0.0007 0.0078 0.0002
Mitochondria 0.0035 0.0011 0.0012 0.0027 0.0013 0.0074 0.0051 0.0031 0.0068 0.0043 0.0041 0.0115 0.0134 0.0008 0.0064 0.0012 0.0237 0.0021
None 0.0543 0.0063 0.0095 0.0729 0.0035 0.0248 0.016 0.0098 0.0057 0.0074 0.0047 0.0074 0.0093 0.0058 0.0056 0.0048 0.0023 0.0038
Nuclear Periphery 0.0258 0.0034 0.0042 0.0097 0.0023 0.0029 0.0079 0.0024 0.004 0.0058 0.0026 0.0035 0.0144 0.0017 0.0045 0.0053 0.01 0.0013
Nucleolus 0.0003 0.0002 0.0005 0.0002 0 0.0001 0.0001 0.0002 0.0001 0 0 0.0003 0.0008 0.0001 0.0001 0 0.0007 0
Nucleus 0.0211 0.0017 0.0033 0.0027 0.0011 0.0022 0.003 0.0023 0.0022 0.0023 0.0021 0.0046 0.0242 0.0012 0.0017 0.0016 0.0035 0.001
Peroxisomes 0.0001 0.0001 0.0005 0.0073 0 0.0001 0.0012 0.0002 0.0004 0.0001 0.0004 0.0004 0.0028 0.0002 0.0072 0.0001 0.0013 0.0003
Punctate Nuclear 0.0022 0.0005 0.0014 0.0019 0.0001 0.0045 0.0007 0.0012 0.0005 0.0004 0.0003 0.0024 0.0024 0.0005 0.0009 0.0003 0.0014 0.0003
Vacuole 0.0069 0.006 0.0036 0.0252 0.0032 0.0047 0.0083 0.0071 0.0047 0.0048 0.0063 0.0079 0.004 0.0028 0.004 0.0036 0.0022 0.0017
Vacuole Periphery 0.0019 0.0008 0.0005 0.0016 0.0008 0.0011 0.0017 0.001 0.001 0.0018 0.0016 0.0024 0.002 0.0006 0.0011 0.0012 0.0026 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.2255 22.3057 18.9163 28.4729 29.6206 25.5861 27.2397 32.3219 28.3776 34.4739
Translational Efficiency 0.7747 0.8295 0.6721 0.559 0.6113 0.7232 0.6359 0.4947 0.5719 0.6151

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative RNA-binding protein; associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Nab6

Nab6


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nab6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available