Standard name
Description Putative RNA-binding protein; associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0.05 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.95 0.92 0.86 0.77 0.7 0.63 0.62 0.51 0.5 0.99 0.99 0.99 0 0 0.77 0.88 0.83 0.83
Endoplasmic Reticulum 0 0 0.08 0.05 0.07 0.06 0.05 0 0.06 0 0 0 0 0 0.11 0.06 0.11 0.09
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0.09 0.29 0.33 0.36 0.47 0.59 0.58 0 0 0 0 0.8 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.07 0.05 0.05 0 0.06 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 3 0 1 3 4 2 1 3 1 3 0 0 0 2 0 3 1
Bud 5 5 2 6 10 5 6 9 9 3 3 7 0 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 8 4 10 10 14 15 27 17 17 29 17 42 0 0 0 0 0 0
Cytoplasm 534 244 313 278 438 418 318 170 206 643 638 650 0 0 469 399 395 455
Endoplasmic Reticulum 13 3 29 17 46 42 24 11 24 9 4 7 0 0 67 29 51 50
Endosome 1 0 2 4 1 7 2 1 1 0 0 0 0 0 3 2 2 6
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 2
Mitochondria 14 32 33 107 207 237 238 197 237 0 0 4 0 0 36 2 5 2
Nucleus 1 0 0 6 2 6 1 1 3 0 1 1 0 0 0 0 0 0
Nuclear Periphery 1 0 2 0 1 4 6 0 3 0 0 0 0 0 1 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 12 3 8 6 42 33 25 11 26 0 1 1 0 0 13 9 5 10
Unique Cell Count 562 266 362 363 629 662 510 336 412 652 647 659 1 1 607 452 477 546
Labelled Cell Count 590 294 399 435 764 771 649 418 529 685 667 712 1 1 607 452 477 546


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.9 6.3 5.8 5.7 5.8 5.2 5.2 4.9 8.3 8.5 7.6 8.7 9.3 9.8
Std Deviation (1e-4) 0.7 0.8 0.9 1.1 1.2 1.1 1.0 0.8 0.9 1.0 1.0 0.9 1.2 1.3 1.4
Intensity Change (Log2) -0.02 0.1 -0.03 -0.05 -0.03 -0.18 -0.18 -0.27 0.49 0.52 0.36 0.56 0.65 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.1 0 0.5 2.1 1.7 0 0 0.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.4 1.4 1.0 1.1 3.6 3.2 2.6 3.1 1.5 4.4
Cytoplasm -1.9 -4.6 -8.4 -11.3 -13.4 -13.2 -15.7 -16.2 3.6 3.6 3.7
Endoplasmic Reticulum -1.2 4.1 2.0 4.0 3.4 2.1 0.9 2.8 -1.2 -2.5 -1.7
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 5.6 4.5 11.9 13.5 14.4 17.0 19.2 19.4 -4.1 -4.0 -2.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.1 -0.5 3.8 2.6 2.5 1.0 3.3 -3.7 -3.3 -3.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nab6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available