Standard name
Description Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.14 0.2 0.38 0.45 0.25 0.26 0.18 0.15 0.1 0.09 0.1 0.2 0.16 0.12 0.05 0.06 0.06 0 0.05 0 0 0 0
Bud 0 0 0.06 0 0 0.07 0.1 0.12 0.12 0.1 0.11 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 0.88 0.87 0.87 0.72 0.55 0.31 0.19 0.14 0.19 0.14 0.87 0.88 0.85 0.85 0.85 0.8 0.81 0.76 0.88 0.82 0.83 0.76
Cytoplasm 0.18 0.13 0.12 0.13 0.17 0.35 0.4 0.49 0.66 0.68 0.66 0.12 0.08 0.1 0.23 0.23 0.24 0.05 0.06 0 0 0 0
Endoplasmic Reticulum 0 0.08 0 0 0.16 0.1 0.12 0.05 0 0 0 0.05 0 0.05 0 0 0 0.07 0.09 0 0.07 0.09 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.12 0.15 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.08 0.14 0.07 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 12 96 108 85 66 78 76 42 26 38 72 77 45 10 20 8 3 4 4 3 7 14
Bud 4 0 15 8 8 18 42 63 51 28 41 12 8 6 10 5 0 2 0 2 3 2 5
Bud Neck 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 1 1 3 3 1 6
Bud Site 0 0 0 0 0 1 4 14 7 3 4 0 0 1 0 0 0
Cell Periphery 139 53 217 208 243 139 132 98 62 53 53 318 423 330 159 307 107 135 79 244 236 378 370
Cytoplasm 28 8 29 31 57 88 169 253 289 191 248 45 37 38 42 82 32 8 6 3 3 6 9
Endoplasmic Reticulum 3 5 3 5 53 25 49 25 5 6 7 17 19 18 7 11 3 11 9 10 20 39 53
Endosome 0 0 1 0 0 2 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 0 1 0 0 0 0 4 22 11 1 1 0 0 0 0 0 0 0
Mitochondria 1 0 2 1 1 5 8 23 52 42 52 0 0 2 6 3 4 1 0 5 2 5 4
Nucleus 1 1 0 0 1 1 8 9 5 1 4 0 1 2 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 2 21 10 6 3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1 3 1 0 1 2 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 3 1 1 1 4 1 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 1 0 2 4 19 34 9 6 12 0 3 2 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 3 9 36 72 33 10 26 1 3 2 0 0 2 1 1 1 9 8 16
Unique Cell Count 155 60 250 239 339 252 425 521 441 279 378 367 482 386 186 362 133 168 105 279 288 454 486
Labelled Cell Count 198 79 366 361 453 359 553 692 567 373 494 473 593 457 235 431 156 168 105 279 288 454 486


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.8 30.0 19.8 21.3 17.6 11.4 9.9 9.0 6.1 6.0 5.6 30.7 30.5 28.4 28.4 34.4 40.7 23.7 21.3 20.1
Std Deviation (1e-4) 7.1 8.7 6.2 6.5 5.6 4.9 4.8 4.3 3.2 3.0 2.9 7.9 8.2 8.5 7.0 9.6 13.0 7.4 5.3 5.8
Intensity Change (Log2) 0.11 -0.17 -0.79 -1.0 -1.14 -1.7 -1.72 -1.83 0.64 0.62 0.52 0.52 0.8 1.04 0.26 0.11 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 -3.5 -2.9 -5.8 -7.4 -9.1 -7.9 -8.5 -5.1 -6.8 -7.9 -8.0 -10.2 -6.8
Bud -1.4 -2.3 0.5 1.8 2.6 2.4 1.7 2.1 -1.6 -3.2 -3.1 -0.3 -3.2 -2.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.1 -4.4 -7.8 -14.0 -18.0 -18.7 -15.6 -18.0 -0.1 0.4 -0.5 -0.4 -0.7 -1.6
Cytoplasm 0.5 1.8 6.2 7.8 10.0 13.7 13.2 13.3 0.2 -1.8 -0.7 3.1 3.5 3.2
Endoplasmic Reticulum 0 5.9 4.3 4.9 2.5 0 0 0 2.4 2.1 2.4 0 1.5 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 3.1 0 0 0 0
Mitochondria 0 0 0 0 2.7 5.2 5.9 5.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 2.8 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3.0 3.8 0 0 2.4 0 0 0 0 0 0
Vacuole 0 0 0 4.7 6.2 4.4 0 4.2 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available