Standard name
Description Component of the mannan polymerase I; complex contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0.07 0.07 0.09 0.1 0.13 0.1 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.27 0.31 0.26 0.29 0.23 0.1 0.08 0.1 0.07 0.07 0.07 0.1 0.21 0.32 0.74 0.35 0.28 0.34 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0.08 0 0 0 0 0 0
Endosome 0 0.15 0.18 0.4 0.45 0.27 0.4 0.33 0.1 0.1 0.05 0.05 0.22 0.37 0.17 0.11 0.19 0.11 0.26 0.14 0.32 0.29 0.16 0.1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.19 0 0 0 0 0 0.11 0.12 0.11 0.15 0.05 0
Mitochondria 0.41 0.1 0.32 0.17 0.22 0.57 0.49 0.39 0.72 0.72 0.81 0.81 0.38 0.18 0 0.06 0 0 0.06 0.08 0.08 0.08 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0.05 0.1 0.1 0.07 0.13 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0.11 0.16 0.05 0.09 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.6 0.62 0.35 0.16 0.15 0.06 0 0 0 0 0 0 0.15 0.23 0.15 0.65 0.66 0.69 0.54 0.62 0.43 0.39 0.65 0.74
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 5 4 7 4 23 16 11 2 5 1 3 0 0 1 0 0 1 2 0 2 2
Bud 1 1 5 9 8 23 27 50 38 48 14 22 1 5 2 0 0 1 0 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 4 3 6 1 2 0 0 0 0 0 0
Cell Periphery 0 1 1 2 1 1 0 3 2 4 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 22 71 62 54 50 35 31 57 27 25 10 19 35 96 293 90 51 46 0 5 3 3 7 4
Endoplasmic Reticulum 5 1 9 6 5 15 9 5 13 6 4 2 11 3 1 7 4 11 1 2 1 3 7 5
Endosome 1 35 44 76 99 93 152 186 38 39 7 10 36 110 67 29 34 15 26 35 90 48 53 23
Golgi 0 2 2 2 6 15 3 14 10 1 3 2 32 8 1 3 0 0 11 30 32 25 16 6
Mitochondria 33 23 78 32 47 194 187 215 278 269 108 160 63 53 14 16 4 3 6 19 24 13 17 10
Nucleus 0 2 0 0 0 1 1 0 1 2 0 0 1 0 0 12 7 1 0 0 0 2 0 1
Nuclear Periphery 1 2 1 0 0 0 0 1 2 0 0 0 1 0 2 0 1 1 0 0 1 1 1 2
Nucleolus 1 2 1 0 1 5 15 20 8 7 3 1 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 1 2 1 7 17 39 58 27 48 3 5 0 4 5 0 1 0 0 0 4 0 0 1
SpindlePole 2 1 7 9 9 15 41 90 21 35 10 7 1 5 2 2 2 1 0 0 0 0 1 0
Vac/Vac Membrane 49 141 86 30 32 19 16 25 9 12 3 8 24 68 61 166 118 93 55 159 122 64 211 178
Unique Cell Count 81 229 243 189 218 340 384 558 385 372 134 197 165 299 395 254 180 135 103 257 286 168 327 242
Labelled Cell Count 115 283 300 226 269 441 526 751 493 513 169 244 206 355 449 325 223 172 103 257 286 168 327 242


Golgi, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 6.6 5.8 6.3 6.6 5.8 5.7 6.2 5.9 5.6 5.5 5.3 6.4 5.7 5.1 6.9 7.9 8.5 6.2 6.4 7.2
Std Deviation (1e-4) 0.8 1.0 1.5 2.5 1.8 0.9 1.6 1.3 2.4 1.4 1.2 1.1 1.1 1.4 1.5 1.6 1.6 1.8 1.5 1.9 2.4
Intensity Change (Log2) 0.1 0.17 -0.01 -0.02 0.09 0.02 -0.06 -0.09 -0.14 0.14 -0.04 -0.18 0.24 0.43 0.55 0.08 0.13 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 2.5 2.4 1.8 0 0 0 0 0 0 0 0
Bud 1.6 1.0 2.6 2.8 3.6 3.8 4.7 3.6 4.0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 -0.6 -4.9 -6.0 -5.6 -6.5 -6.5 -4.3 -4.3 -1.0 1.7 12.0 2.4 0.6 1.8
Endoplasmic Reticulum -0.3 -0.9 0.4 -1.0 0 -0.2 -1.6 0 0 1.4 -2.1 0 -0.6 -0.9 1.8
Endosome 5.1 6.3 2.6 5.7 4.4 -3.0 -2.7 -3.5 -4.1 0.9 4.8 -0.4 -2.1 0.2 -1.8
Golgi 0 0 2.5 0 0 0 0 0 0 6.7 0 0 0 0 0
Mitochondria -3.6 -2.5 6.0 4.1 1.7 9.9 9.8 9.0 10.3 1.3 -3.9 -10.0 -7.3 -7.7 -6.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 3.4 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.7 2.6 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 2.8 4.6 4.7 3.6 5.4 0 0 0 0 0 0 0 0
SpindlePole 1.0 0.7 1.0 3.6 5.3 1.5 3.1 2.1 0.4 0 -1.0 0 0 0 0
Vacuole -4.5 -5.1 -9.2 -10.3 -11.6 -11.3 -10.7 -7.3 -8.0 -4.7 -3.2 -5.8 6.7 6.1 6.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Van1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available