Standard name
Description DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0.06 0.12 0.16 0.11 0.10 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.08 0 0 0 0.05 0.09 0.08 0.40 0.26 0.24 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.96 0.93 0.95 0.92 0.92 0.90 0.88 0.83 0.82 0.81 0.80 0.87 0.86 0.80 0.76 0.78 0.87 0.95 0.91 0.92 0.85 0.74 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0.10 0.10 0.11 0.12 0 0 0 0.05 0 0.07 0 0 0 0 0.05 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0.06 0.14 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 3 3 2 2 5 0 0 0 0 0 0 0 2 1 0 0 2
Bud Neck 0 0 0 0 0 0 1 5 2 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 2 2 6 9 10 16 17 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 5 8 6 12 11 11 19 7 14 12 12 6 23 33 7 6 2 0 1 1 1 3 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0 0 0 3
Endosome 0 1 2 0 4 0 3 1 0 0 0 0 1 0 1 2 1 0 0 2 2 1 0 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 19 1 1 0 18 32 33 127 103 84 131 1 1 1 0 1 2 1 1 0 0 0 3
Nucleus 116 232 231 186 233 308 319 353 265 323 284 333 82 163 165 48 47 65 123 224 230 85 113 79
Nuclear Periphery 0 0 0 0 1 4 3 2 4 6 2 6 0 1 1 0 0 2 0 0 0 0 1 2
Nucleolus 4 0 3 1 0 6 11 15 31 40 39 49 1 1 2 3 0 5 0 2 2 2 7 19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 1 1 3 9 6 3 5 3 8 0 1 3 0 0 1 0 1 0 0 2 3
Vac/Vac Membrane 3 0 4 1 7 2 6 6 2 4 5 2 4 3 7 3 4 2 2 6 8 6 21 41
Unique Cell Count 123 242 248 195 254 333 355 401 321 396 349 418 94 189 206 63 60 75 130 247 250 101 154 172
Labelled Cell Count 129 258 250 196 258 355 397 449 453 508 447 563 95 193 216 64 60 81 130 247 250 101 154 172

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 6.1 8.3 7.8 7.4 5.8 5.5 5.8 4.6 4.9 5.0 4.8 7.8 7.4 7.1 8.8 10.0 10.3 9.1 9.2 8.7
Std Deviation (1e-4) 2.8 1.1 1.4 1.4 1.6 1.1 1.2 1.4 1.1 1.1 1.1 0.9 1.6 1.5 1.7 4.1 3.1 3.0 1.4 1.4 1.8
Intensity Change (Log2) -0.08 -0.17 -0.52 -0.6 -0.51 -0.85 -0.74 -0.74 -0.8 -0.09 -0.17 -0.21 0.1 0.28 0.32 0.14 0.15 0.08

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.4 4.6 4.3 0 8.6 8.2 0 0 0 0 0 0 0
Nucleus 0 3.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1.5 1.9 0 4.4 4.7 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0