Standard name
Description Alpha 2 subunit of the 20S proteasome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.05 0 0 0.06 0.06 0 0.05 0 0 0 0 0.08 0.1 0.05 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.79 0.75 0.79 0.69 0.82 0.68 0.71 0.73 0.75 0.78 0.72 0.87 0.83 0.76 0.64 0.59 0.55 0.77 0.75 0.61 0.77 0.64 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.15 0.24 0.23 0.42 0.11 0.23 0.16 0.28 0.11 0.32 0.45 0 0.11 0.11 0 0.06 0.1 0.17 0.1 0.25 0.14 0.14 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.05 0.09 0.08 0.17 0.08 0.1 0 0 0.09 0 0.09 0.21 0.27 0.21 0 0.11 0.09 0.07 0.19 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 9 2 3 0 0 4 7 1 4 0 0 0 6 9 12 5 15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 1
Mitochondria 0 2 1 2 0 0 5 0 3 2 2 1 0 0 0 1 4 1 0 0 0 0 1 2
Nucleus 18 104 56 48 41 80 45 81 62 65 29 29 20 117 89 74 57 56 40 114 64 79 91 85
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4 20 18 14 25 11 15 18 24 10 12 18 1 15 13 5 6 10 9 15 26 14 19 40
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Vac/Vac Membrane 0 7 0 2 3 9 5 19 7 9 1 1 2 4 11 24 26 21 2 16 9 7 27 49
Unique Cell Count 19 132 75 61 59 97 66 114 85 87 37 40 23 141 117 116 97 101 52 153 105 103 143 188
Labelled Cell Count 22 143 77 69 69 101 76 127 97 90 44 49 23 142 123 119 102 109 52 153 105 103 143 188


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 49.6 38.7 43.8 37.3 23.1 36.3 19.4 35.4 33.5 36.1 31.7 32.8 46.1 40.2 41.9 34.0 38.8 35.8 38.3 42.0 41.4
Std Deviation (1e-4) 13.6 13.8 13.9 13.8 8.8 11.2 9.0 12.7 10.2 10.1 7.9 10.2 17.2 16.2 15.8 16.9 19.6 17.8 17.1 15.2 16.9
Intensity Change (Log2) – – – -0.23 -0.93 -0.27 -1.17 -0.31 -0.39 -0.28 -0.47 -0.42 0.07 -0.12 -0.06 -0.36 -0.17 -0.29 -0.19 -0.06 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 2.0 0 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.5 -0.7 1.2 -0.9 -0.5 -0.2 0 0.4 -0.3 1.2 1.5 0.2 -1.6 -2.2 -2.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.1 2.3 -2.2 -0.2 -1.4 0.6 -2.1 0.9 2.3 0 -2.6 -2.4 -4.1 -3.3 -2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 0 0 0 0 0 0 0 4.2 4.9 4.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0251 0 0.0001 0.0078 0.0017 0.0067 0 0 0.004 0.0001 0 0 0 0.0002 0.0001 0 0.0445 0.0009
Bud 0.0009 0 0 0.0014 0.0008 0.0002 0 0 0.0047 0 0 0.0001 0 0.0001 0.0001 0 0.0005 0.0004
Bud Neck 0.0004 0.0001 0.0001 0.0003 0.0007 0.0004 0 0 0.0002 0 0 0.0002 0 0.0001 0 0 0.0003 0.0002
Bud Periphery 0.0015 0 0.0001 0.0015 0.0008 0.0005 0 0 0.0013 0 0 0.0001 0 0 0.0001 0 0.0008 0.0007
Bud Site 0.0019 0 0 0.005 0.0009 0.0001 0 0.0001 0.0092 0 0 0 0 0.0003 0.0002 0 0.0006 0.0006
Cell Periphery 0.0004 0.0002 0.0002 0.0003 0.0023 0.0004 0 0 0.0001 0 0 0.0002 0 0.0001 0 0 0.0004 0.0003
Cytoplasm 0.0029 0.0001 0.0018 0.0528 0.0046 0.0002 0 0 0.0144 0.0004 0 0 0.0001 0.0116 0.041 0.0001 0.0009 0.0001
Cytoplasmic Foci 0.0273 0 0 0.0006 0.0269 0.0023 0 0 0.0039 0 0 0 0 0.0013 0.0004 0 0.0037 0.0011
Eisosomes 0.0004 0 0 0.0002 0.0007 0 0 0 0 0 0 0 0 0 0 0 0.0004 0.0002
Endoplasmic Reticulum 0.0026 0 0.0006 0.0006 0.0012 0.001 0 0 0.0008 0 0 0 0 0.0034 0.0004 0 0.0019 0
Endosome 0.0275 0 0.0004 0.0018 0.0519 0.0065 0 0 0.0004 0.0001 0 0 0 0.0221 0.0016 0 0.0115 0.0001
Golgi 0.0106 0 0 0.0007 0.0065 0.0055 0 0 0.0002 0 0 0 0 0.0004 0 0 0.0079 0.0002
Lipid Particles 0.0354 0 0 0.0006 0.0146 0.0179 0 0 0.0002 0 0 0 0 0.0009 0 0 0.0173 0.002
Mitochondria 0.0379 0.0015 0.0023 0.0237 0.0362 0.0035 0.0025 0.0022 0.0025 0.001 0.0002 0.0009 0.0022 0.003 0.0027 0.0018 0.0221 0.0083
None 0.0036 0 0.0001 0.0008 0.0084 0.0002 0 0 0.0017 0.0001 0 0 0 0.0017 0.0002 0 0.0026 0.0003
Nuclear Periphery 0.0573 0.0049 0.0179 0.0123 0.1033 0.0463 0.0007 0.0085 0.006 0.0826 0 0.0003 0.0014 0.0215 0.0093 0.0011 0.1081 0.001
Nucleolus 0.1313 0.0882 0.0727 0.0347 0.1998 0.2135 0.0187 0.1786 0.0161 0.0679 0.1701 0.1772 0.0267 0.0514 0.0692 0.0528 0.1975 0.2813
Nucleus 0.591 0.9035 0.8988 0.849 0.4899 0.6864 0.9777 0.8097 0.9084 0.8383 0.8295 0.8199 0.969 0.8725 0.8707 0.9439 0.5489 0.698
Peroxisomes 0.0073 0 0 0.0006 0.0029 0.0004 0 0 0.0003 0 0 0 0 0 0 0 0.0029 0.001
Punctate Nuclear 0.0198 0.0001 0.0003 0.0024 0.0076 0.0032 0 0.0003 0.0242 0.0079 0.0001 0.0002 0 0.001 0.0004 0 0.0189 0.0012
Vacuole 0.0072 0.0004 0.001 0.0013 0.0171 0.0011 0 0.0001 0.0004 0.0001 0 0.0003 0 0.0064 0.0019 0 0.0012 0.0006
Vacuole Periphery 0.0077 0.001 0.0034 0.0018 0.0214 0.0037 0.0002 0.0003 0.0008 0.0012 0 0.0006 0.0004 0.0021 0.0016 0.0002 0.0071 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 132.6137 103.4844 120.5309 126.7115 94.9942 127.4144 143.3736 144.816 150.719 119.8179
Translational Efficiency 1.8103 2.3405 2.0068 2.1236 1.9659 2.2207 1.9891 1.9588 1.8523 1.8833

Hit Data

Dataset Hit
Protein Abundance –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘