Standard name
Description Alpha 2 subunit of the 20S proteasome

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.07 0 0.05 0 0 0.06 0.06 0 0.05 0 0 0 0 0.08 0.1 0.05 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.08 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.79 0.75 0.79 0.69 0.82 0.68 0.71 0.73 0.75 0.78 0.72 0.87 0.83 0.76 0.64 0.59 0.55 0.77 0.75 0.61 0.77 0.64 0.45
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.15 0.24 0.23 0.42 0.11 0.23 0.16 0.28 0.11 0.32 0.45 0 0.11 0.11 0 0.06 0.1 0.17 0.1 0.25 0.14 0.14 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.05 0.09 0.08 0.17 0.08 0.1 0 0 0.09 0 0.09 0.21 0.27 0.21 0 0.11 0.09 0.07 0.19 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 9 2 3 0 0 4 7 1 4 0 0 0 6 9 12 5 15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2 5 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 1
Mitochondria 0 2 1 2 0 0 5 0 3 2 2 1 0 0 0 1 4 1 0 0 0 0 1 2
Nucleus 18 104 56 48 41 80 45 81 62 65 29 29 20 117 89 74 57 56 40 114 64 79 91 85
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4 20 18 14 25 11 15 18 24 10 12 18 1 15 13 5 6 10 9 15 26 14 19 40
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Vac/Vac Membrane 0 7 0 2 3 9 5 19 7 9 1 1 2 4 11 24 26 21 2 16 9 7 27 49
Unique Cell Count 19 132 75 61 59 97 66 114 85 87 37 40 23 141 117 116 97 101 52 153 105 103 143 188
Labelled Cell Count 22 143 77 69 69 101 76 127 97 90 44 49 23 142 123 119 102 109 52 153 105 103 143 188


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 49.6 38.7 43.8 37.3 23.1 36.3 19.4 35.4 33.5 36.1 31.7 32.8 46.1 40.2 41.9 34.0 38.8 35.8 38.3 42.0 41.4
Std Deviation (1e-4) 13.6 13.8 13.9 13.8 8.8 11.2 9.0 12.7 10.2 10.1 7.9 10.2 17.2 16.2 15.8 16.9 19.6 17.8 17.1 15.2 16.9
Intensity Change (Log2) -0.23 -0.93 -0.27 -1.17 -0.31 -0.39 -0.28 -0.47 -0.42 0.07 -0.12 -0.06 -0.36 -0.17 -0.29 -0.19 -0.06 -0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 2.0 0 2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.5 -0.7 1.2 -0.9 -0.5 -0.2 0 0.4 -0.3 1.2 1.5 0.2 -1.6 -2.2 -2.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.1 2.3 -2.2 -0.2 -1.4 0.6 -2.1 0.9 2.3 0 -2.6 -2.4 -4.1 -3.3 -2.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 3.7 0 0 0 0 0 0 0 4.2 4.9 4.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pre8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available