Standard name
Human Ortholog
Description Subunit of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 0.99 0.97 0.9 0.83 0.8 0.76 0.73 0.71 0.69 0.99 0.99 0.99 – – – 0.87 0.87 0.74 0.76 0.83 0.74
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.1 0.24 0.36 0.39 0.43 0.39 0.51 0.51 0 0 0 – – – 0.05 0 0.11 0.12 0.07 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – 4 2 7 2 1 4
Bud 0 2 1 0 1 6 8 11 9 7 12 11 0 0 0 – – – 0 6 2 1 0 5
Bud Neck 1 2 8 2 4 16 9 2 5 6 4 6 0 0 1 – – – 1 2 0 1 0 0
Bud Site 0 0 0 0 0 4 2 4 4 5 2 9 0 0 0 – – – – – – – – –
Cell Periphery 2 2 2 1 1 1 1 1 6 2 3 3 0 0 0 – – – 1 2 2 2 0 1
Cytoplasm 424 403 346 382 372 482 493 610 400 397 387 389 182 192 242 – – – 378 350 245 143 109 133
Endoplasmic Reticulum 1 0 1 0 0 0 1 0 0 0 0 0 1 0 2 – – – 1 0 3 1 1 1
Endosome 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 2 3 4 2 0 3
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – 2 1 1 0 0 0
Mitochondria 6 4 6 8 38 130 210 300 227 214 279 286 0 0 0 – – – 23 13 35 22 9 11
Nucleus 0 1 1 2 0 3 2 6 1 6 5 1 0 2 0 – – – 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 3 1 1 1 0 0 0 0 – – – 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 0 0 2 2 8 11 12 17 27 13 21 0 0 0 – – – 3 7 8 0 0 1
Vac/Vac Membrane 5 3 0 0 3 1 2 2 2 2 3 2 0 0 1 – – – 7 3 1 9 3 11
Unique Cell Count 432 412 353 386 385 535 591 762 523 543 548 560 183 193 244 436 405 330 189 131 181
Labelled Cell Count 440 420 367 397 421 652 739 951 672 668 709 728 183 194 246 436 405 330 189 131 181


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 3.7 3.1 3.3 3.0 2.9 2.5 2.6 2.4 2.5 2.3 2.4 4.7 4.4 4.6 4.5 4.8 4.9 5.3 5.3 5.2
Std Deviation (1e-4) 0.6 1.1 1.0 2.1 1.6 1.4 1.3 1.3 1.4 1.2 0.9 1.1 1.0 0.8 1.3 1.8 1.7 1.4 1.6 2.4 1.3
Intensity Change (Log2) – – – 0.1 -0.04 -0.07 -0.3 -0.23 -0.34 -0.34 -0.46 -0.36 0.6 0.52 0.57 0.53 0.62 0.65 0.76 0.78 0.75


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0772 0.3479 0.4079 0.3003 0.1067 0.1814 1.2689 1.2978 1.7531 1.5111 1.1693 1.7075 -0.6104 -1.2448 -1.5377 -0.7096 -1.6104 -1.4204
Actin 0.0002 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0002 0 0.0001 0.0252 0.0004 0.0013 0.0062 0.0001 0.0011
Bud 0 0.0001 0.0013 0.0148 0 0.0042 0 0.0002 0.0006 0 0 0.0001 0.0015 0.0001 0.0016 0.0033 0.0001 0.0007
Bud Neck 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0 0.0003 0.0001 0 0.0001 0.0018 0.0001 0.0001 0.003 0 0.0266
Bud Periphery 0.0001 0.0001 0.0003 0.0024 0 0.0011 0.0001 0.0004 0.0006 0.0001 0 0.0002 0.0025 0.0002 0.0063 0.0128 0.0001 0.0008
Bud Site 0.0003 0.0025 0.0006 0.0079 0 0.0007 0.0005 0.0008 0.0037 0.0001 0 0 0.0212 0.0014 0.0012 0.0095 0 0.0001
Cell Periphery 0 0.0001 0 0.0019 0 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0006 0.0003 0.0003 0.0005 0 0
Cytoplasm 0.0081 0.0141 0.0082 0.0044 0.0068 0.0151 0.0115 0.0222 0.0117 0.0253 0.0084 0.0266 0.0098 0.018 0.0158 0.0109 0.0229 0.024
Cytoplasmic Foci 0.003 0.002 0.0013 0.0004 0.0018 0.002 0.0011 0.0011 0.0011 0.003 0.0004 0.0023 0.0639 0.0119 0.0095 0.035 0.0027 0.0086
Eisosomes 0.0001 0.0001 0.0001 0.0001 0 0 0.0002 0.0001 0.0001 0 0 0.0001 0.0005 0.0004 0.0004 0.0005 0.0001 0.0001
Endoplasmic Reticulum 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0002 0.0002 0.0003 0 0.0001 0.002 0.0001 0.0004 0.0135 0.0015 0.0005
Endosome 0.0012 0.0002 0.0002 0 0 0.0001 0 0.0001 0.0001 0.0022 0 0.0004 0.042 0.0002 0.0007 0.0276 0.0814 0.0105
Golgi 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0216 0.0002 0.0005 0.0077 0.0007 0.0007
Lipid Particles 0.0002 0 0.0001 0 0.0001 0 0 0.0001 0 0.0002 0 0.0001 0.034 0.0007 0.0005 0.0018 0.0001 0.0009
Mitochondria 0.0001 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0001 0.0002 0.0001 0 0.0001 0.0407 0.0001 0.0006 0.0152 0.0005 0.0009
None 0.9839 0.9725 0.9827 0.9601 0.9743 0.9713 0.9854 0.9725 0.9752 0.9664 0.9908 0.9689 0.6928 0.963 0.9581 0.8242 0.8694 0.8968
Nuclear Periphery 0.0001 0.0002 0.0002 0.0001 0.0002 0.0002 0 0.0011 0.0003 0.0002 0 0.0001 0.0022 0 0.0002 0.0117 0.0021 0.0004
Nucleolus 0.0002 0.0005 0.0003 0.0017 0.0008 0.0002 0.0001 0.0001 0.0007 0.0001 0 0.0001 0.0026 0.0002 0.0001 0.0004 0 0.0001
Nucleus 0.0003 0.0045 0.0012 0.0038 0.0015 0.0017 0.0002 0.0004 0.0025 0.0005 0.0001 0.0003 0.0012 0.0002 0.0003 0.0013 0.0004 0.0003
Peroxisomes 0.0001 0.0001 0.0001 0 0 0 0 0 0.0001 0 0 0 0.0236 0.0013 0.0009 0.0016 0.0001 0.0229
Punctate Nuclear 0.0019 0.0023 0.0031 0.0001 0.0141 0.0022 0.0002 0.0002 0.0022 0.0004 0.0001 0.0003 0.0033 0.0012 0.0008 0.0018 0.0002 0.0003
Vacuole 0.0001 0.0002 0.0001 0.0017 0.0001 0.0003 0.0001 0.0002 0.0001 0.0007 0 0.0002 0.0054 0.0001 0.0003 0.0071 0.0117 0.0022
Vacuole Periphery 0 0 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0.0015 0 0.0001 0.0045 0.0058 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 107.2754 65.7584 130.1215 110.1457 95.3741 110.2794 124.6655 106.5784 103.4579 118.9545
Translational Efficiency 2.5692 4.1306 1.7616 2.3664 3.1074 3.0817 2.202 2.6356 2.7646 2.5019

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘