ORF
Description Non-essential protein of unknown function; has structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to the nucleus and cytoplasm

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.89 0.97 0.55 0.99 0.99 0.99 0.93 0.94 0.96 0.95 0.85 0.92 0.89 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.4 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0 0.3 0 0 0 0.07 0.08 0 0 0.09 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 1 1 0 2 3 3
Bud 1 2 0 0 0 0 0 0 0 0 2 0 1 6 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0
Cell Periphery 2 1 1 0 0 1 15 12 11 0 0 0 0 0 0
Cytoplasm 186 322 11 77 269 310 402 378 252 253 295 24 133 360 244
Endoplasmic Reticulum 0 1 0 0 2 1 13 6 8 0 1 0 2 3 2
Endosome 0 0 0 0 1 0 8 2 0 1 3 0 1 3 6
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 3 3 8 0 0 0 3 3 3 2 3 0 1 5 1
Nucleus 7 3 0 0 0 0 4 4 3 0 2 0 0 0 1
Nuclear Periphery 5 0 0 1 0 0 0 0 0 0 0 0 1 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3
Vac/Vac Membrane 12 5 6 2 1 5 29 33 7 1 32 1 1 1 1
Unique Cell Count 210 331 20 78 272 314 432 403 262 266 349 27 151 396 279
Labelled Cell Count 217 337 26 80 273 317 474 439 285 266 349 27 151 396 279


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 6.0 5.5 6.8 6.8 7.3 8.1 8.7 8.1 5.9 6.0 6.4
Std Deviation (1e-4) 1.0 1.1 1.2 1.5 1.4 1.3 1.3 1.4 1.2 1.8 1.4 1.9
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole