Standard name
Description GTP-binding protein of the Ras superfamily; involved in termination of M-phase; controls actomyosin and septin dynamics during cytokinesis

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.06 0 0.06 0.08 0.09 0.13 0.1 0.13 0.1 0 0 0 0 0 0 0.06 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.05 0 0 0 0 0 0 0 0 0
Bud Site 0.06 0 0.05 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.39 0.48 0.47 0.58 0.7 0.68 0.54 0.54 0.47 0.46 0.46 0.39 0.77 0.79 0.77 0.28 0.26 0.29 0.22 0.32 0.22 0.51 0.66 0.7
Endoplasmic Reticulum 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0.21 0.08 0.12 0.06 0 0 0 0 0 0 0 0 0 0.05 0.28 0.37 0.34 0 0.09 0.06 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0 0.26 0.1 0.17 0.3 0.45 0.4 0.49 0.41 0.44 0.49 0 0 0 0.05 0 0 0 0 0 0.07 0.07 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0.05 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.39 0.11 0.26 0.25 0.14 0.07 0.17 0.18 0.19 0.24 0.2 0.2 0.07 0.13 0.15 0.48 0.48 0.45 0.56 0.31 0.52 0.14 0.06 0.05
Vac/Vac Membrane 0 0.15 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.09 0.11 0.1 0 0.05 0 0.09 0.07 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 1 0 0 1 0 1 1 2 0 1 6 6 9 3 3 2 0 4 1 4 6 5
Bud 1 5 3 13 13 19 37 36 49 53 43 53 4 2 7 3 5 5 4 8 1 6 9 9
Bud Neck 0 2 0 2 6 10 11 11 10 15 6 13 14 23 23 0 3 0 0 2 0 2 3 3
Bud Site 2 0 4 3 4 8 14 15 22 19 12 19 0 3 1 4 6 2
Cell Periphery 0 0 0 1 0 0 0 0 1 2 1 0 0 0 2 0 0 1 0 0 0 0 2 1
Cytoplasm 13 71 34 124 220 207 246 228 179 249 155 207 175 361 392 49 56 47 15 58 23 87 254 282
Endoplasmic Reticulum 2 2 0 0 0 0 0 0 0 1 0 1 2 3 6 7 4 5 0 2 0 0 4 3
Endosome 2 31 6 26 18 0 1 4 0 6 4 10 5 14 25 50 82 56 2 16 6 2 6 14
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 3 1 1 0 3
Mitochondria 8 3 19 21 52 92 204 167 186 224 147 262 1 1 0 8 3 1 2 6 3 12 28 29
Nucleus 0 6 1 0 1 2 3 4 3 1 3 1 11 1 1 3 2 1 1 9 5 8 6 0
Nuclear Periphery 0 2 0 0 0 0 1 0 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 1 0 3 1 0 0 1 1 1 0 4 0 0 0 3 8 3 0 0 1 0 0 0
Peroxisomes 0 1 0 7 2 2 1 3 2 7 10 10 0 1 1 4 9 9 1 1 0 0 0 0
SpindlePole 13 16 19 54 45 21 78 77 72 133 66 107 16 58 77 85 105 74 40 56 55 23 24 18
Vac/Vac Membrane 1 22 1 3 3 2 1 3 3 6 2 4 13 7 4 16 24 16 1 9 3 15 26 11
Unique Cell Count 33 147 73 214 315 303 454 419 380 547 337 533 226 459 508 176 219 164 73 184 107 172 384 401
Labelled Cell Count 43 167 88 257 365 364 597 550 530 720 450 693 247 481 548 235 310 222 73 184 107 172 384 401


Punctate Nuclear, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.4 5.4 3.7 3.5 3.4 3.2 2.9 3.2 2.9 2.9 2.8 2.8 5.0 4.9 5.0 6.9 6.8 7.2 5.5 5.2 5.3
Std Deviation (1e-4) 2.6 1.3 0.7 1.3 0.9 1.3 1.3 1.4 0.7 0.9 0.9 0.7 1.0 1.1 0.7 1.4 1.3 1.7 1.7 1.2 1.8
Intensity Change (Log2) -0.06 -0.14 -0.19 -0.37 -0.22 -0.37 -0.34 -0.39 -0.38 0.45 0.41 0.44 0.9 0.87 0.96 0.58 0.5 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 3.8 3.5 1.2 1.2 0.1 -0.2 -0.1 -1.3 5.0 5.8 5.5 -2.9 -3.4 -2.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 3.5 4.7 4.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -5.0 -6.9 -6.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 -4.4 -3.0 -2.3 3.2 3.3 2.8
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5 2.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tem1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available