Standard name
Human Ortholog
Description Nuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.05 0.18 0.23 0.19 0.13 0.11 0.11 0.09 0.11 0.08 0.07 0.34 0.31 0.39 0.09 0.05 0.05 0 0 0.05 0.11 0.18 0.26
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.49 0.19 0 0.11 0.1 0.5 0.62 0.41 0.67 0.54 0.71 0.53 0 0 0 0 0 0.06 0 0 0 0 0 0
Nucleus 0.67 0.87 0.77 0.67 0.7 0.62 0.56 0.66 0.62 0.58 0.58 0.67 0.62 0.62 0.52 0.82 0.87 0.86 0.67 0.75 0.62 0.48 0.35 0.22
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0.17 0.11 0.2 0.24 0.25 0.29
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 5
Bud 3 0 0 0 1 2 9 3 6 7 14 6 0 0 0 1 0 1 5 1 6 2 8 10
Bud Neck 0 0 5 2 5 2 2 6 5 1 1 3 2 3 0 0 0 0 1 0 1 0 7 6
Bud Site 0 0 0 1 1 8 5 5 18 13 20 20 0 0 1 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 1 1 0 0 0 2 1 0 0 0 0 0 0 0 0 0
Cytoplasm 30 3 47 65 78 56 66 59 46 48 45 37 78 72 96 21 16 17 16 1 17 19 63 87
Endoplasmic Reticulum 1 0 0 1 0 0 1 1 2 0 0 1 1 0 0 3 2 5 2 0 1 1 1 1
Endosome 4 0 1 5 14 2 2 2 0 3 0 1 0 5 12 3 3 3 7 2 2 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 2 0 0 0 0 0
Mitochondria 189 12 8 32 40 211 357 225 343 243 391 274 7 3 1 4 5 19 8 1 8 7 15 14
Nucleus 258 54 200 190 288 261 321 368 315 262 322 346 142 146 128 199 275 267 313 63 209 83 121 74
Nuclear Periphery 1 0 1 0 0 1 3 1 2 0 1 3 0 0 0 3 4 4 1 0 0 0 1 1
Nucleolus 4 1 0 0 0 0 0 2 2 6 7 2 0 2 0 3 9 13 2 0 1 4 9 3
Peroxisomes 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0
SpindlePole 4 0 5 13 12 9 8 16 10 7 4 14 5 2 1 4 3 1 16 2 14 6 19 21
Vac/Vac Membrane 13 1 3 8 13 7 10 16 3 13 9 16 5 9 15 15 14 9 81 9 67 41 87 97
Unique Cell Count 387 62 261 284 413 424 574 554 509 452 554 514 229 234 246 242 317 310 470 85 337 173 345 341
Labelled Cell Count 508 71 270 318 452 559 784 704 753 604 814 723 240 244 255 259 334 341 470 85 337 173 345 341


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.8 4.9 4.7 4.9 3.9 3.6 4.3 3.6 4.0 3.4 4.0 4.8 5.4 5.2 9.8 9.2 8.8 4.8 5.0 5.5
Std Deviation (1e-4) 0.6 0.8 0.7 1.6 1.8 1.4 1.0 1.3 1.3 1.7 1.1 1.1 1.1 1.7 1.6 1.9 2.1 2.1 1.5 1.3 2.0
Intensity Change (Log2) – – – -0.05 0.02 -0.33 -0.44 -0.18 -0.43 -0.29 -0.51 -0.3 -0.02 0.14 0.1 1.0 0.91 0.85 -0.02 0.03 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.0 2.3 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4776 1.5197 1.8138 1.3022 1.555 1.3233 0.7953 0.5381 0.9875 1.5746 0.9656 0.5848 1.0127 0.5189 0.5611 0.7724 0.6928 0.2808
Actin 0.0177 0 0.0109 0 0.0475 0.0013 0.009 0.0001 0.0153 0.0266 0.0059 0.002 0.0107 0 0.0004 0.0065 0.0005 0.0007
Bud 0.0007 0 0.0003 0 0.0012 0.0002 0.0006 0 0.0002 0.0021 0.0003 0.0002 0.0002 0.0001 0 0.0002 0 0
Bud Neck 0.0018 0.0001 0.0009 0.0001 0.0011 0.0013 0.0086 0.0002 0.0015 0.008 0.0008 0.0016 0.0046 0.0003 0.0018 0.0045 0.0008 0.0008
Bud Periphery 0.0014 0 0.0004 0 0.004 0.0006 0.0029 0 0.0006 0.0061 0.0005 0.0003 0.0007 0.0001 0 0.0006 0 0
Bud Site 0.0013 0.0005 0.0044 0 0.0031 0.0002 0.007 0.0001 0.0029 0.0185 0.0013 0.0003 0.0006 0.0004 0.0004 0.0009 0 0.0001
Cell Periphery 0.0001 0 0.0001 0 0.0008 0.0001 0.0014 0 0.0005 0.0011 0.0001 0 0.0002 0 0 0.0002 0 0
Cytoplasm 0.0072 0.0042 0.0024 0.0002 0.0016 0.001 0.0198 0.0095 0.0027 0.0032 0.0049 0.0023 0.0105 0.0217 0.0041 0.0042 0.008 0.003
Cytoplasmic Foci 0.0076 0.0001 0.0028 0 0.002 0.0006 0.0025 0.0001 0.0013 0.0051 0.0061 0.0022 0.0085 0.0005 0.0003 0.0007 0.0001 0.0002
Eisosomes 0.0003 0 0.0001 0 0.0002 0 0.0005 0 0.0004 0.0005 0.0002 0 0.0001 0 0 0.0003 0.0001 0
Endoplasmic Reticulum 0.0018 0.0001 0.0006 0 0.002 0.0002 0.0047 0.0003 0.0044 0.006 0.0026 0.0001 0.0027 0.0007 0.0004 0.0012 0.0004 0.0003
Endosome 0.0103 0.0001 0.0046 0 0.0113 0.0019 0.0104 0.0003 0.005 0.032 0.0274 0.0016 0.0089 0.0014 0.0007 0.0041 0.0006 0.0014
Golgi 0.0037 0 0.0015 0 0.0109 0.0007 0.0017 0 0.0035 0.0095 0.0066 0.0008 0.0013 0 0.0001 0.0015 0 0.0002
Lipid Particles 0.0073 0 0.0041 0 0.0073 0.0029 0.0061 0 0.0042 0.0102 0.0182 0.0029 0.0027 0.0001 0.0001 0.0012 0.0001 0.0004
Mitochondria 0.0021 0.0002 0.0072 0.0001 0.0203 0.0051 0.0104 0.0003 0.0089 0.007 0.0105 0.0008 0.0023 0.0003 0.0002 0.0022 0.0004 0.0002
None 0.0202 0.0029 0.0021 0.0001 0.0008 0.0003 0.0124 0.0135 0.0105 0.0022 0.0052 0.001 0.0205 0.0178 0.0053 0.0008 0.0171 0.0016
Nuclear Periphery 0.0156 0.0024 0.0087 0.0008 0.0093 0.0031 0.0234 0.0079 0.0064 0.0295 0.0753 0.0021 0.0265 0.0062 0.0036 0.0115 0.0141 0.0035
Nucleolus 0.0075 0.0099 0.0065 0.0038 0.0188 0.0162 0.0159 0.0084 0.007 0.0072 0.0187 0.0187 0.0103 0.0069 0.0056 0.0079 0.0089 0.0123
Nucleus 0.8739 0.9721 0.9205 0.9939 0.8278 0.9423 0.8295 0.946 0.909 0.7728 0.7076 0.9413 0.8712 0.9214 0.9664 0.9331 0.9318 0.9653
Peroxisomes 0.0036 0 0.0038 0 0.0016 0.0021 0.0009 0 0.0009 0.0026 0.0339 0.0064 0.0018 0 0 0.0002 0 0
Punctate Nuclear 0.0139 0.0071 0.0175 0.001 0.0194 0.0188 0.0238 0.0127 0.0128 0.036 0.0696 0.0148 0.0126 0.02 0.0102 0.0163 0.0163 0.0095
Vacuole 0.001 0.0001 0.0003 0 0.0057 0.0007 0.0065 0.0003 0.0012 0.01 0.002 0.0002 0.0018 0.0017 0.0003 0.0011 0.0002 0.0002
Vacuole Periphery 0.0008 0 0.0002 0 0.0035 0.0005 0.0019 0.0002 0.0007 0.0039 0.0026 0.0001 0.0013 0.0003 0.0001 0.0009 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 43.4142 18.3147 7.1187 11.7888 27.1955 36.0772 10.1408 4.4637 9.1647 25.6227
Translational Efficiency 1.5629 1.0808 1.3824 1.5028 1.4111 1.4444 1.1845 1.7579 1.2616 1.3867

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Nuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ogg1

Ogg1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ogg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available