Standard name | |
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Description | Nuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 5 |
Bud | 3 | 0 | 0 | 0 | 1 | 2 | 9 | 3 | 6 | 7 | 14 | 6 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 1 | 6 | 2 | 8 | 10 |
Bud Neck | 0 | 0 | 5 | 2 | 5 | 2 | 2 | 6 | 5 | 1 | 1 | 3 | 2 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 7 | 6 |
Bud Site | 0 | 0 | 0 | 1 | 1 | 8 | 5 | 5 | 18 | 13 | 20 | 20 | 0 | 0 | 1 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 3 | 47 | 65 | 78 | 56 | 66 | 59 | 46 | 48 | 45 | 37 | 78 | 72 | 96 | 21 | 16 | 17 | 16 | 1 | 17 | 19 | 63 | 87 |
Endoplasmic Reticulum | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 2 | 5 | 2 | 0 | 1 | 1 | 1 | 1 |
Endosome | 4 | 0 | 1 | 5 | 14 | 2 | 2 | 2 | 0 | 3 | 0 | 1 | 0 | 5 | 12 | 3 | 3 | 3 | 7 | 2 | 2 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 189 | 12 | 8 | 32 | 40 | 211 | 357 | 225 | 343 | 243 | 391 | 274 | 7 | 3 | 1 | 4 | 5 | 19 | 8 | 1 | 8 | 7 | 15 | 14 |
Nucleus | 258 | 54 | 200 | 190 | 288 | 261 | 321 | 368 | 315 | 262 | 322 | 346 | 142 | 146 | 128 | 199 | 275 | 267 | 313 | 63 | 209 | 83 | 121 | 74 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 1 | 2 | 0 | 1 | 3 | 0 | 0 | 0 | 3 | 4 | 4 | 1 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 6 | 7 | 2 | 0 | 2 | 0 | 3 | 9 | 13 | 2 | 0 | 1 | 4 | 9 | 3 |
Peroxisomes | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 4 | 0 | 5 | 13 | 12 | 9 | 8 | 16 | 10 | 7 | 4 | 14 | 5 | 2 | 1 | 4 | 3 | 1 | 16 | 2 | 14 | 6 | 19 | 21 |
Vac/Vac Membrane | 13 | 1 | 3 | 8 | 13 | 7 | 10 | 16 | 3 | 13 | 9 | 16 | 5 | 9 | 15 | 15 | 14 | 9 | 81 | 9 | 67 | 41 | 87 | 97 |
Unique Cell Count | 387 | 62 | 261 | 284 | 413 | 424 | 574 | 554 | 509 | 452 | 554 | 514 | 229 | 234 | 246 | 242 | 317 | 310 | 470 | 85 | 337 | 173 | 345 | 341 |
Labelled Cell Count | 508 | 71 | 270 | 318 | 452 | 559 | 784 | 704 | 753 | 604 | 814 | 723 | 240 | 244 | 255 | 259 | 334 | 341 | 470 | 85 | 337 | 173 | 345 | 341 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 3.6 | 4.8 | 4.9 | 4.7 | 4.9 | 3.9 | 3.6 | 4.3 | 3.6 | 4.0 | 3.4 | 4.0 | 4.8 | 5.4 | 5.2 | 9.8 | 9.2 | 8.8 | 4.8 | 5.0 | 5.5 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.7 | 1.6 | 1.8 | 1.4 | 1.0 | 1.3 | 1.3 | 1.7 | 1.1 | 1.1 | 1.1 | 1.7 | 1.6 | 1.9 | 2.1 | 2.1 | 1.5 | 1.3 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.02 | -0.33 | -0.44 | -0.18 | -0.43 | -0.29 | -0.51 | -0.3 | -0.02 | 0.14 | 0.1 | 1.0 | 0.91 | 0.85 | -0.02 | 0.03 | 0.17 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.0 | 2.3 | 1.5 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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