Standard name
Description Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.34 0.34 0.29 0.29 0.07 0.19 0.09 0.05 0.08 0.06 0 0.4 0.4 0.38 0.38 0.38 0.38 0.35 0.36 0.45 0.38 0.37 0.41
Bud 0 0 0.12 0.11 0.15 0.11 0.12 0.12 0.13 0.16 0.08 0.07 0.09 0.1 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.07 0.08 0.1 0.1 0.05 0 0 0 0 0.05 0.05 0.1 0.11 0.22 0.1 0.13 0.1 0 0 0.06 0.07 0.07 0.11
Cytoplasm 0.14 0.17 0.14 0.17 0.13 0.18 0.18 0.2 0.1 0.1 0.15 0.2 0.19 0.19 0.22 0.16 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.41 0.53 0.28 0.16 0.17 0.22 0.15 0.07 0.13 0.08 0 0.42 0.33 0.23 0.05 0.05 0.07 0.43 0.34 0.21 0.24 0.25 0.2
Endosome 0.05 0 0 0 0.07 0 0.05 0 0 0 0 0 0 0 0.07 0.05 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0.27 0.21 0 0 0.05 0 0 0
Mitochondria 0.1 0.16 0.3 0.55 0.38 0.57 0.6 0.62 0.76 0.82 0.81 0.11 0.16 0.21 0.08 0.15 0.08 0.08 0.09 0.11 0.12 0.12 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0.11 0.11 0.33 0.07 0.1 0.1 0.07 0.05 0.05 0 0 0 0.11 0.09 0.18 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.09 0.14 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 77 127 79 73 20 53 39 26 40 20 3 50 111 122 62 64 73 110 159 156 69 111 139
Bud 10 14 33 29 42 31 50 68 61 53 31 9 24 31 3 4 5 0 2 0 0 2 1
Bud Neck 1 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 0 2 16 5 10 7 9
Bud Site 1 3 1 1 1 5 4 4 7 6 5 0 1 1 0 0 1
Cell Periphery 16 32 26 26 14 12 14 15 18 17 21 13 30 70 16 22 20 11 14 20 12 20 38
Cytoplasm 32 63 37 43 38 52 77 115 49 34 60 25 53 60 37 27 33 2 6 1 3 8 6
Endoplasmic Reticulum 91 201 77 41 50 62 63 40 61 25 17 53 91 73 8 8 13 134 152 73 44 76 68
Endosome 12 2 12 6 20 5 21 23 3 0 10 1 4 0 11 9 16 4 11 10 5 6 9
Golgi 5 3 2 0 0 0 0 0 0 0 0 1 0 0 24 46 40 9 16 18 5 6 9
Mitochondria 23 62 82 139 109 164 259 352 367 271 321 14 45 67 14 26 15 25 38 37 21 37 35
Nucleus 1 0 1 2 1 0 0 3 1 0 1 2 0 0 0 2 1 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 15 5 29 29 96 20 43 58 36 18 18 4 12 10 18 15 35 4 3 16 2 7 7
SpindlePole 2 2 8 2 11 3 10 27 10 9 7 0 4 3 15 23 22 0 0 0 0 0 0
Vac/Vac Membrane 10 7 7 0 2 1 4 10 2 5 11 0 0 1 5 5 11 3 15 8 6 13 8
Unique Cell Count 224 377 271 254 289 286 429 569 480 332 394 126 279 318 165 168 191 313 443 352 184 304 338
Labelled Cell Count 296 521 394 391 404 409 584 743 655 460 508 172 376 438 213 251 286 313 443 352 184 304 338


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 6.0 5.4 5.1 5.1 4.8 4.6 4.4 4.0 3.9 3.7 5.5 5.7 5.4 8.6 9.3 9.9 6.3 6.3 6.6
Std Deviation (1e-4) 1.4 1.0 0.8 1.3 1.6 1.4 1.5 1.4 1.1 1.1 0.8 1.0 0.9 0.9 1.7 2.1 2.3 1.2 1.3 1.4
Intensity Change (Log2) -0.06 -0.07 -0.16 -0.22 -0.29 -0.41 -0.45 -0.54 0.05 0.08 0.02 0.69 0.8 0.88 0.24 0.24 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.1 -6.9 0 0 0 0 0 0 2.1 2.6 2.4 1.8 1.9 2.0
Bud -0.3 0.8 0 0 0 0 0 0 -1.5 -1.4 -0.9 -3.8 -3.6 -3.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.2 -2.2 0 0 0 0 0 0 0.2 0.4 4.1 0 1.1 0.3
Cytoplasm 1.0 -0.2 1.5 1.5 2.3 -1.4 -1.3 0.6 1.6 1.7 1.7 2.4 0.7 1.1
Endoplasmic Reticulum -3.4 -3.1 0 0 0 0 0 0 2.7 1.1 -1.5 -6.0 -6.1 -5.8
Endosome -1.3 1.3 0 0 0 0 0 0 0 -2.1 -3.8 1.0 0.4 1.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 5.9 8.7 7.4
Mitochondria 5.7 1.9 0 0 0 0 0 0 -4.1 -3.9 -2.6 -5.3 -3.5 -5.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.3 6.4 0 0 0 0 0 0 -2.5 -2.9 -3.7 0.1 -0.6 2.3
SpindlePole 0 0.6 0 0 0 0 0 0 0 -1.2 -1.8 2.8 4.3 3.7
Vacuole 0 0 0 0 -0.8 0 -0.9 0.2 0 0 0 0 0 1.7


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nte1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available