Standard name | |
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Description | Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 96 | 246 | 216 | 46 | 110 | 82 | 92 | 85 | 119 | 131 | 94 | 94 | 213 | 201 | 248 | 56 | 52 | 66 | 149 | 410 | 257 | 188 | 349 | 387 |
Bud | 22 | 58 | 63 | 21 | 63 | 41 | 50 | 39 | 32 | 57 | 37 | 40 | 32 | 33 | 42 | 19 | 19 | 10 | 3 | 17 | 3 | 14 | 17 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6 | 25 | 9 | 13 | 12 | 7 |
Bud Site | 3 | 10 | 30 | 11 | 8 | 6 | 2 | 1 | 12 | 13 | 3 | 4 | 3 | 0 | 2 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 46 | 82 | 70 | 10 | 19 | 15 | 30 | 29 | 54 | 53 | 35 | 34 | 155 | 197 | 185 | 69 | 35 | 58 | 34 | 27 | 26 | 23 | 37 | 45 |
Cytoplasm | 9 | 28 | 8 | 3 | 15 | 13 | 9 | 22 | 8 | 11 | 4 | 8 | 13 | 12 | 18 | 68 | 30 | 37 | 1 | 6 | 2 | 1 | 0 | 1 |
Endoplasmic Reticulum | 3 | 66 | 18 | 3 | 13 | 13 | 29 | 28 | 19 | 18 | 8 | 10 | 24 | 41 | 47 | 15 | 13 | 14 | 4 | 22 | 12 | 6 | 10 | 7 |
Endosome | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 16 | 6 | 3 | 3 | 3 |
Golgi | 54 | 65 | 16 | 2 | 17 | 9 | 21 | 26 | 18 | 18 | 19 | 15 | 103 | 109 | 63 | 197 | 160 | 100 | 1 | 19 | 3 | 2 | 2 | 6 |
Mitochondria | 5 | 22 | 110 | 55 | 63 | 67 | 108 | 79 | 134 | 168 | 133 | 134 | 20 | 9 | 63 | 58 | 23 | 24 | 7 | 17 | 7 | 6 | 10 | 11 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 3 | 0 | 1 | 0 | 2 | 2 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 |
Nucleolus | 4 | 8 | 0 | 1 | 1 | 9 | 7 | 8 | 5 | 8 | 2 | 5 | 1 | 1 | 0 | 10 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 3 | 18 | 4 | 12 | 3 | 7 | 1 | 4 | 8 | 2 | 10 | 5 | 3 | 8 | 0 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | 2 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 2 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 4 | 10 | 5 | 0 | 3 | 1 | 5 | 8 | 9 | 7 | 2 | 2 | 2 | 0 | 0 | 10 | 1 | 3 | 1 | 8 | 1 | 1 | 1 | 1 |
Unique Cell Count | 157 | 393 | 286 | 79 | 178 | 143 | 223 | 194 | 217 | 279 | 190 | 211 | 351 | 369 | 395 | 357 | 238 | 241 | 215 | 581 | 338 | 267 | 449 | 491 |
Labelled Cell Count | 246 | 599 | 557 | 156 | 325 | 259 | 362 | 329 | 414 | 494 | 341 | 358 | 573 | 608 | 676 | 506 | 342 | 325 | 215 | 581 | 338 | 267 | 449 | 491 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 26.0 | 22.2 | 13.4 | 13.1 | 15.4 | 15.9 | 16.1 | 15.6 | 15.3 | 15.1 | 14.1 | 14.4 | 26.3 | 27.9 | 24.9 | 37.8 | 40.8 | 38.7 | 24.0 | 25.1 | 25.5 |
Std Deviation (1e-4) | 7.7 | 5.9 | 3.2 | 3.1 | 4.2 | 4.2 | 3.8 | 4.1 | 4.1 | 4.1 | 3.8 | 4.7 | 6.5 | 7.2 | 6.7 | 15.3 | 14.4 | 13.4 | 5.9 | 5.9 | 6.4 |
Intensity Change (Log2) | – | – | – | -0.03 | 0.21 | 0.25 | 0.27 | 0.23 | 0.2 | 0.18 | 0.08 | 0.1 | 0.98 | 1.06 | 0.9 | 1.5 | 1.61 | 1.53 | 0.85 | 0.91 | 0.93 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -3.0 | -3.1 | -3.9 | -7.8 | -7.1 | -4.9 | -7.0 | -5.8 | -7.0 | -4.0 | -5.6 | -3.5 | -15.3 | -12.2 | -11.0 |
Bud | 0.9 | 3.1 | 1.5 | 0.1 | -0.5 | -2.1 | -0.5 | -0.7 | -0.8 | -4.5 | -4.7 | -4.1 | -6.3 | -4.4 | -5.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.9 | -2.3 | -2.2 | -4.4 | -4.4 | -2.0 | -2.6 | -3.7 | -3.8 | -5.5 | -6.4 | -6.1 | -6.3 | -5.1 | -4.9 |
Cell Periphery | -2.2 | -3.7 | -3.4 | -3.1 | -2.5 | 0.1 | -1.6 | -1.6 | -2.3 | 5.2 | 7.5 | 6.0 | -1.6 | -2.8 | -0.1 |
Cytoplasm | 0 | 2.7 | 2.8 | 0.8 | 3.8 | 0.6 | 0.8 | 0 | 0.6 | 0.6 | 0.3 | 1.2 | 6.3 | 4.3 | 5.1 |
Endoplasmic Reticulum | 0 | 0.4 | 1.1 | 2.6 | 3.0 | 1.0 | 0.1 | -1.0 | -0.7 | 0.3 | 2.1 | 2.5 | -1.2 | -0.4 | -0.2 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 1.6 | 0.3 | 1.6 | 3.0 | 1.2 | 0.4 | 1.8 | 0.7 | 7.6 | 7.7 | 4.2 | 13.3 | 14.9 | 9.9 |
Mitochondria | 4.9 | -0.7 | 1.7 | 2.3 | 0.5 | 5.2 | 5.2 | 6.7 | 5.5 | -10.2 | -11.9 | -6.7 | -6.4 | -7.5 | -7.5 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0.2 | -1.9 | -1.6 | -3.2 | -2.4 | -1.9 | -2.8 | -0.7 | -3.3 | -3.9 | -2.9 | -4.8 | -3.6 | -3.3 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.6 | 1.6 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0.9 | 0 | 0 |