Standard name
Description Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.61 0.63 0.76 0.58 0.62 0.57 0.41 0.44 0.55 0.47 0.49 0.45 0.61 0.54 0.63 0.16 0.22 0.27 0.7 0.71 0.76 0.71 0.78 0.79
Bud 0.14 0.15 0.22 0.27 0.35 0.29 0.22 0.2 0.15 0.2 0.19 0.19 0.09 0.09 0.11 0.05 0.08 0 0 0 0 0.06 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Site 0 0 0.1 0.14 0 0 0 0 0.06 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.29 0.21 0.24 0.13 0.11 0.1 0.13 0.15 0.25 0.19 0.18 0.16 0.44 0.53 0.47 0.19 0.15 0.24 0.16 0.05 0.08 0.09 0.08 0.09
Cytoplasm 0.06 0.07 0 0 0.08 0.09 0 0.11 0 0 0 0 0 0 0.05 0.19 0.13 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.17 0.06 0 0.07 0.09 0.13 0.14 0.09 0.06 0 0.05 0.07 0.11 0.12 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.34 0.17 0.06 0 0.1 0.06 0.09 0.13 0.08 0.06 0.1 0.07 0.29 0.3 0.16 0.55 0.67 0.41 0 0 0 0 0 0
Mitochondria 0 0.06 0.38 0.7 0.35 0.47 0.48 0.41 0.62 0.6 0.7 0.64 0.06 0 0.16 0.16 0.1 0.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.06 0.05 0.07 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 96 246 216 46 110 82 92 85 119 131 94 94 213 201 248 56 52 66 149 410 257 188 349 387
Bud 22 58 63 21 63 41 50 39 32 57 37 40 32 33 42 19 19 10 3 17 3 14 17 12
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 6 25 9 13 12 7
Bud Site 3 10 30 11 8 6 2 1 12 13 3 4 3 0 2 0 0 1 – – – – – –
Cell Periphery 46 82 70 10 19 15 30 29 54 53 35 34 155 197 185 69 35 58 34 27 26 23 37 45
Cytoplasm 9 28 8 3 15 13 9 22 8 11 4 8 13 12 18 68 30 37 1 6 2 1 0 1
Endoplasmic Reticulum 3 66 18 3 13 13 29 28 19 18 8 10 24 41 47 15 13 14 4 22 12 6 10 7
Endosome 0 1 0 0 0 0 0 1 0 1 1 0 1 0 0 1 1 1 1 16 6 3 3 3
Golgi 54 65 16 2 17 9 21 26 18 18 19 15 103 109 63 197 160 100 1 19 3 2 2 6
Mitochondria 5 22 110 55 63 67 108 79 134 168 133 134 20 9 63 58 23 24 7 17 7 6 10 11
Nucleus 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 1 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 3 0 1 0 2 2 0 0 0 1 0 1 0 0 0 0 0 3 0 1 0 0
Nucleolus 4 8 0 1 1 9 7 8 5 8 2 5 1 1 0 10 5 5 0 0 0 0 0 0
Peroxisomes 0 3 18 4 12 3 7 1 4 8 2 10 5 3 8 0 1 2 0 0 2 0 0 2
SpindlePole 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 2 2 4 0 0 0 0 0 0
Vac/Vac Membrane 4 10 5 0 3 1 5 8 9 7 2 2 2 0 0 10 1 3 1 8 1 1 1 1
Unique Cell Count 157 393 286 79 178 143 223 194 217 279 190 211 351 369 395 357 238 241 215 581 338 267 449 491
Labelled Cell Count 246 599 557 156 325 259 362 329 414 494 341 358 573 608 676 506 342 325 215 581 338 267 449 491


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 26.0 22.2 13.4 13.1 15.4 15.9 16.1 15.6 15.3 15.1 14.1 14.4 26.3 27.9 24.9 37.8 40.8 38.7 24.0 25.1 25.5
Std Deviation (1e-4) 7.7 5.9 3.2 3.1 4.2 4.2 3.8 4.1 4.1 4.1 3.8 4.7 6.5 7.2 6.7 15.3 14.4 13.4 5.9 5.9 6.4
Intensity Change (Log2) – – – -0.03 0.21 0.25 0.27 0.23 0.2 0.18 0.08 0.1 0.98 1.06 0.9 1.5 1.61 1.53 0.85 0.91 0.93


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -3.0 -3.1 -3.9 -7.8 -7.1 -4.9 -7.0 -5.8 -7.0 -4.0 -5.6 -3.5 -15.3 -12.2 -11.0
Bud 0.9 3.1 1.5 0.1 -0.5 -2.1 -0.5 -0.7 -0.8 -4.5 -4.7 -4.1 -6.3 -4.4 -5.9
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.9 -2.3 -2.2 -4.4 -4.4 -2.0 -2.6 -3.7 -3.8 -5.5 -6.4 -6.1 -6.3 -5.1 -4.9
Cell Periphery -2.2 -3.7 -3.4 -3.1 -2.5 0.1 -1.6 -1.6 -2.3 5.2 7.5 6.0 -1.6 -2.8 -0.1
Cytoplasm 0 2.7 2.8 0.8 3.8 0.6 0.8 0 0.6 0.6 0.3 1.2 6.3 4.3 5.1
Endoplasmic Reticulum 0 0.4 1.1 2.6 3.0 1.0 0.1 -1.0 -0.7 0.3 2.1 2.5 -1.2 -0.4 -0.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 1.6 0.3 1.6 3.0 1.2 0.4 1.8 0.7 7.6 7.7 4.2 13.3 14.9 9.9
Mitochondria 4.9 -0.7 1.7 2.3 0.5 5.2 5.2 6.7 5.5 -10.2 -11.9 -6.7 -6.4 -7.5 -7.5
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.2 -1.9 -1.6 -3.2 -2.4 -1.9 -2.8 -0.7 -3.3 -3.9 -2.9 -4.8 -3.6 -3.3
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 1.6 1.6 0.6 0 0 0 0 0 0.9 0 0