Standard name | |
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Description | Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 2 | 1 | 1 | 0 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | – | 4 | 5 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 2 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | – | 5 | 3 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 6 | 8 | 10 | 7 | 14 | 7 | 12 | – | 10 | 14 | 3 | 4 | 26 | 18 | 20 | 33 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 1 | 0 | 10 | 5 | 13 | 3 | 6 | 10 | – | 11 | 6 | 7 | 1 | 0 | 2 | 6 | 5 | 3 | 0 | 0 | 0 | 0 | 3 | 4 |
Endosome | 5 | 0 | 14 | 1 | 23 | 22 | 44 | 58 | – | 8 | 3 | 14 | 4 | 13 | 8 | 20 | 27 | 13 | 1 | 2 | 10 | 5 | 13 | 25 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | – | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 3 | 6 | 10 |
Mitochondria | 17 | 28 | 74 | 39 | 61 | 178 | 221 | 246 | – | 302 | 222 | 320 | 2 | 2 | 0 | 17 | 28 | 13 | 2 | 3 | 6 | 19 | 52 | 98 |
Nucleus | 4 | 9 | 7 | 11 | 3 | 13 | 20 | 22 | – | 18 | 25 | 25 | 12 | 5 | 5 | 30 | 41 | 11 | 1 | 3 | 1 | 2 | 3 | 3 |
Nuclear Periphery | 93 | 121 | 245 | 129 | 151 | 148 | 216 | 255 | – | 256 | 166 | 259 | 71 | 219 | 126 | 87 | 140 | 65 | 118 | 140 | 335 | 276 | 370 | 386 |
Nucleolus | 38 | 26 | 103 | 47 | 100 | 131 | 215 | 316 | – | 230 | 166 | 219 | 30 | 44 | 39 | 65 | 139 | 60 | 1 | 4 | 6 | 7 | 11 | 9 |
Peroxisomes | 0 | 0 | 1 | 0 | 0 | 1 | 9 | 8 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 | 4 |
SpindlePole | 0 | 0 | 0 | 0 | 2 | 1 | 8 | 8 | – | 5 | 1 | 4 | 0 | 2 | 1 | 1 | 5 | 0 | 0 | 0 | 2 | 1 | 3 | 3 |
Vac/Vac Membrane | 23 | 27 | 35 | 17 | 26 | 26 | 34 | 42 | – | 20 | 14 | 23 | 10 | 60 | 41 | 41 | 61 | 23 | 3 | 11 | 10 | 12 | 15 | 18 |
Unique Cell Count | 127 | 161 | 341 | 192 | 255 | 378 | 545 | 708 | 574 | 421 | 588 | 101 | 282 | 187 | 213 | 339 | 138 | 134 | 169 | 379 | 334 | 500 | 581 | |
Labelled Cell Count | 183 | 217 | 501 | 260 | 388 | 540 | 786 | 988 | 872 | 626 | 879 | 134 | 374 | 241 | 290 | 480 | 194 | 134 | 169 | 379 | 334 | 500 | 581 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 12.8 | 12.2 | 11.4 | 11.2 | 10.9 | 9.3 | 8.8 | 9.1 | – | 7.8 | 7.6 | 7.9 | 11.8 | 13.0 | 14.0 | 8.9 | 10.3 | 13.3 | 14.6 | 14.7 | 14.7 |
Std Deviation (1e-4) | 3.7 | 3.5 | 4.5 | 3.7 | 4.4 | 3.7 | 3.4 | 3.5 | – | 3.3 | 3.5 | 3.4 | 2.9 | 3.3 | 4.0 | 3.0 | 3.7 | 4.0 | 3.0 | 2.9 | 3.0 |
Intensity Change (Log2) | – | – | – | -0.03 | -0.06 | -0.3 | -0.38 | -0.33 | – | -0.55 | -0.59 | -0.54 | 0.05 | 0.18 | 0.29 | -0.37 | -0.15 | 0.22 | 0.35 | 0.36 | 0.36 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.8 | 0.3 | 1.1 | -1.2 | -0.7 | – | -0.6 | 0.8 | 0 | 0 | 3.8 | 3.7 | 3.7 | 4.0 | 0 |
Endoplasmic Reticulum | -0.2 | 1.4 | -2.1 | -2.0 | -1.7 | – | -1.0 | -1.4 | -1.9 | 0 | 0 | 0 | -0.1 | -1.3 | 0 |
Endosome | -2.4 | 2.5 | 1.1 | 2.3 | 2.5 | – | -2.6 | -3.2 | -1.5 | 0 | 0.3 | 0.1 | 2.5 | 2.1 | 2.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.4 | 0.6 | 7.1 | 5.8 | 4.3 | – | 9.2 | 8.7 | 9.7 | -4.6 | -8.0 | -6.9 | -4.2 | -4.9 | -3.2 |
Nucleus | 2.3 | 0 | 1.1 | 1.4 | 1.0 | – | 1.0 | 2.7 | 1.8 | 0 | -0.3 | 0 | 5.5 | 5.1 | 3.1 |
Nuclear Periphery | -1.1 | -3.2 | -8.8 | -9.3 | -10.9 | – | -8.0 | -8.9 | -8.2 | -0.3 | 1.7 | -1.1 | -7.2 | -8.0 | -5.1 |
Nucleolus | -1.4 | 2.3 | 1.3 | 2.8 | 4.5 | – | 3.0 | 2.6 | 2.2 | -0.1 | -4.3 | -2.3 | 0.1 | 2.9 | 2.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.5 | 0 | -1.6 | -2.2 | -2.5 | – | -4.2 | -3.9 | -3.9 | -0.1 | 3.8 | 3.7 | 3.0 | 2.9 | 1.9 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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