Standard name
Human Ortholog
Description Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.95 0.98 0.94 0.92 0.85 0.73 0.78 0.61 0.65 0.53 0.55 0.94 0.97 0.98 0.96 0.97 0.96 0.78 0.79 0.77 0.91 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0 0.05 0.2 0.41 0.28 0.54 0.36 0.61 0.51 0 0 0 0 0 0 0.07 0.07 0.12 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 3 3 2 0 0 0
Bud 2 1 0 1 2 2 5 6 5 6 8 9 0 0 0 0 0 1 6 2 1 0 0 3
Bud Neck 3 0 3 7 1 4 2 5 3 5 1 4 0 1 0 1 1 1 2 0 0 0 0 1
Bud Site 1 0 0 0 0 0 0 0 4 0 2 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 2 1 1 1 0 0 3 0 1 0 1 2 6 9 7 0 0 1 0 0 0
Cytoplasm 274 212 271 291 347 374 344 358 254 261 212 189 17 57 48 419 401 364 231 187 196 30 51 82
Endoplasmic Reticulum 5 4 2 2 0 2 0 0 0 2 0 0 0 0 1 14 22 29 3 0 4 0 0 0
Endosome 0 0 1 1 1 2 0 1 0 0 0 0 0 1 0 4 1 1 3 5 2 0 0 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 1 1 2 1 0 0 0
Mitochondria 32 6 0 5 19 88 194 127 226 146 247 173 0 0 0 4 6 8 20 16 30 2 1 2
Nucleus 2 1 1 3 4 8 9 11 15 22 19 26 0 0 0 0 2 1 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
SpindlePole 0 0 1 3 4 2 5 3 3 9 2 4 0 0 0 1 1 0 7 2 1 0 0 0
Vac/Vac Membrane 1 2 1 3 17 11 25 11 15 16 19 20 1 2 0 6 10 6 3 8 4 0 0 1
Unique Cell Count 299 222 276 310 379 442 474 461 419 401 402 342 18 59 49 435 415 378 296 238 257 33 56 97
Labelled Cell Count 321 229 282 319 396 495 586 522 527 470 511 431 18 62 51 460 455 420 296 238 257 33 56 97


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.1 4.7 4.4 4.4 3.7 3.5 4.2 3.3 3.6 3.1 3.5 4.0 4.8 5.1 6.3 5.8 5.3 – 4.5 5.3
Std Deviation (1e-4) 1.1 0.9 1.6 1.6 1.3 1.4 1.3 1.5 1.3 1.1 1.0 0.9 0.6 0.6 1.6 1.7 1.9 1.6 – 1.6 2.5
Intensity Change (Log2) – – – -0.1 -0.11 -0.34 -0.43 -0.17 -0.5 -0.37 -0.59 -0.44 -0.23 0.02 0.12 0.41 0.29 0.18 – -0.07 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2569 0.4495 0.2936 0.4403 0.6057 0.2933 -0.4299 -0.0188 -0.2019 -0.1803 -0.9744 0.0268 -0.3668 -0.7649 -0.5303 -0.6574 -0.4082 -0.5859
Actin 0.0215 0.0007 0.005 0.0005 0.0368 0.0024 0.0022 0.0004 0.0027 0.0001 0.0001 0.0003 0.0238 0.0004 0.0046 0.0024 0.0346 0.0083
Bud 0.0025 0.0052 0.0001 0.0001 0.0012 0.0029 0.005 0.0013 0.0052 0 0 0.0009 0.0019 0.0062 0.0017 0.0004 0.0002 0.0015
Bud Neck 0.004 0.0002 0.0003 0.0027 0.0035 0.0015 0.0029 0.0006 0.0006 0 0.0001 0.0035 0.0054 0.002 0.0173 0.0017 0.0004 0.0017
Bud Periphery 0.0041 0.0031 0.0005 0.0001 0.0018 0.0027 0.0017 0.0014 0.003 0.0001 0 0.0002 0.0027 0.0014 0.0031 0.0014 0.0003 0.001
Bud Site 0.0079 0.0025 0.0002 0.0002 0.0162 0.0003 0.0027 0.0078 0.0062 0 0 0.0004 0.0033 0.0132 0.0086 0.0008 0.0002 0.0002
Cell Periphery 0.0008 0.0001 0.0001 0.0001 0.0003 0.0001 0.0003 0.0001 0.0003 0 0 0.0001 0.0006 0.0003 0.0004 0.0005 0.0001 0.0001
Cytoplasm 0.0698 0.1185 0.0768 0.0706 0.0446 0.1132 0.0325 0.0627 0.0498 0.0322 0.0451 0.0866 0.0513 0.0857 0.0665 0.0431 0.0807 0.1674
Cytoplasmic Foci 0.0298 0.0043 0.005 0.0067 0.02 0.0113 0.0035 0.0046 0.0036 0.0061 0.0069 0.0074 0.0185 0.0071 0.0061 0.0049 0.0134 0.0083
Eisosomes 0.0005 0.0001 0.0002 0.0002 0.0006 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0.0004 0.0001 0.0002 0.0005 0.0003 0.0001
Endoplasmic Reticulum 0.0047 0.0008 0.0008 0.0017 0.0024 0.0009 0.0031 0.0005 0.0006 0.0003 0.0003 0.0004 0.0051 0.0007 0.0013 0.0008 0.0033 0.0025
Endosome 0.0167 0.0009 0.0009 0.0007 0.0167 0.0014 0.0042 0.0027 0.0011 0.0014 0.0006 0.0017 0.0191 0.0012 0.0016 0.0004 0.013 0.0052
Golgi 0.0062 0.0001 0.0002 0.0001 0.0087 0.0005 0.0005 0.0005 0.0001 0.0001 0 0.0001 0.0026 0.0001 0.0005 0.0002 0.003 0.0008
Lipid Particles 0.0217 0.0001 0.0005 0.0005 0.0203 0.0022 0.0006 0.0005 0.0002 0.0004 0.0004 0.0007 0.0029 0.0002 0.0008 0.0004 0.0019 0.0007
Mitochondria 0.0066 0.0003 0.0032 0.0005 0.034 0.0015 0.0007 0.0009 0.0008 0.0005 0.0002 0.0005 0.0025 0.0003 0.0014 0.0005 0.0019 0.0007
None 0.7589 0.8421 0.8845 0.9007 0.7377 0.8253 0.9227 0.8969 0.9093 0.9478 0.8956 0.8726 0.8212 0.8732 0.8609 0.9371 0.8049 0.7662
Nuclear Periphery 0.0047 0.0009 0.0012 0.001 0.0099 0.0012 0.0042 0.0008 0.0008 0.0002 0.0015 0.0009 0.011 0.0004 0.0045 0.0005 0.0291 0.0099
Nucleolus 0.001 0.001 0.0005 0.0002 0.0054 0.001 0.0005 0.0015 0.0015 0.0003 0.0006 0.0012 0.0011 0.0004 0.0012 0.0005 0.0005 0.0009
Nucleus 0.0045 0.0114 0.016 0.0098 0.0078 0.0131 0.0049 0.0066 0.0068 0.0011 0.0265 0.007 0.0116 0.0034 0.0108 0.0009 0.0031 0.0099
Peroxisomes 0.0246 0.0002 0.0011 0.0012 0.0174 0.0038 0.0013 0.0007 0.0003 0.0002 0.0001 0.0007 0.004 0.0002 0.0011 0.0006 0.0009 0.0003
Punctate Nuclear 0.0065 0.0067 0.0021 0.0013 0.0123 0.0139 0.0019 0.0086 0.0055 0.0082 0.0214 0.014 0.0079 0.0027 0.006 0.0018 0.0063 0.0131
Vacuole 0.0021 0.0005 0.0005 0.0008 0.0014 0.0006 0.0037 0.0008 0.0011 0.0008 0.0002 0.0005 0.002 0.0006 0.001 0.0004 0.0008 0.0009
Vacuole Periphery 0.0009 0.0001 0.0001 0.0001 0.0014 0.0002 0.0009 0.0001 0.0003 0.0001 0.0001 0.0001 0.0014 0.0001 0.0003 0.0001 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.4146 3.4885 3.2358 6.1354 1.2649 12.6758 4.6295 4.7613 5.2814 5.4461
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Rsf1

Rsf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rsf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available