Standard name
Description Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.95 0.98 0.94 0.92 0.85 0.73 0.78 0.61 0.65 0.53 0.55 0.94 0.97 0.98 0.96 0.97 0.96 0.78 0.79 0.77 0.91 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0 0 0 0.05 0.2 0.41 0.28 0.54 0.36 0.61 0.51 0 0 0 0 0 0 0.07 0.07 0.12 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0.05 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 3 3 2 0 0 0
Bud 2 1 0 1 2 2 5 6 5 6 8 9 0 0 0 0 0 1 6 2 1 0 0 3
Bud Neck 3 0 3 7 1 4 2 5 3 5 1 4 0 1 0 1 1 1 2 0 0 0 0 1
Bud Site 1 0 0 0 0 0 0 0 4 0 2 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 2 2 1 1 1 0 0 3 0 1 0 1 2 6 9 7 0 0 1 0 0 0
Cytoplasm 274 212 271 291 347 374 344 358 254 261 212 189 17 57 48 419 401 364 231 187 196 30 51 82
Endoplasmic Reticulum 5 4 2 2 0 2 0 0 0 2 0 0 0 0 1 14 22 29 3 0 4 0 0 0
Endosome 0 0 1 1 1 2 0 1 0 0 0 0 0 1 0 4 1 1 3 5 2 0 0 0
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 1 1 2 1 0 0 0
Mitochondria 32 6 0 5 19 88 194 127 226 146 247 173 0 0 0 4 6 8 20 16 30 2 1 2
Nucleus 2 1 1 3 4 8 9 11 15 22 19 26 0 0 0 0 2 1 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0
SpindlePole 0 0 1 3 4 2 5 3 3 9 2 4 0 0 0 1 1 0 7 2 1 0 0 0
Vac/Vac Membrane 1 2 1 3 17 11 25 11 15 16 19 20 1 2 0 6 10 6 3 8 4 0 0 1
Unique Cell Count 299 222 276 310 379 442 474 461 419 401 402 342 18 59 49 435 415 378 296 238 257 33 56 97
Labelled Cell Count 321 229 282 319 396 495 586 522 527 470 511 431 18 62 51 460 455 420 296 238 257 33 56 97


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.3 4.1 4.7 4.4 4.4 3.7 3.5 4.2 3.3 3.6 3.1 3.5 4.0 4.8 5.1 6.3 5.8 5.3 – 4.5 5.3
Std Deviation (1e-4) 1.1 0.9 1.6 1.6 1.3 1.4 1.3 1.5 1.3 1.1 1.0 0.9 0.6 0.6 1.6 1.7 1.9 1.6 – 1.6 2.5
Intensity Change (Log2) – – – -0.1 -0.11 -0.34 -0.43 -0.17 -0.5 -0.37 -0.59 -0.44 -0.23 0.02 0.12 0.41 0.29 0.18 – -0.07 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –