Standard name
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p); NUP116 has a paralog, NUP100, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.97 0.94 0.9 0.77 0.76 0.64 0.63 0.61 0.58 1.0 0.97 0.99 – 0.97 0.96 0.9 0.92 0.84 0.85 0.82 0.85
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.09 0 0.25 0.27 0.31 0.49 0.51 0.44 0.5 0 0 0 – 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.07 0.07 0.07 0.1 0.07 0.09 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 1 1 0 1 2
Bud 0 1 0 0 0 0 4 5 6 3 3 6 0 0 0 – 0 0 5 1 2 2 4 7
Bud Neck 0 0 0 0 0 0 0 1 1 0 0 1 0 0 1 – 2 0 1 0 0 1 0 1
Bud Site 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 – 0 0 – – – – – –
Cell Periphery 1 0 0 1 1 1 8 3 3 2 6 5 0 0 0 – 2 4 0 0 0 0 0 0
Cytoplasm 268 204 208 138 165 180 244 247 154 194 134 165 58 58 72 – 271 343 255 196 170 84 150 152
Endoplasmic Reticulum 4 1 3 0 1 0 1 0 2 1 3 1 0 2 0 – 18 27 1 1 2 1 1 0
Endosome 1 0 1 0 2 0 3 0 1 2 1 3 0 0 0 – 2 1 3 0 3 0 3 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 3 0 1 0 0 0 0
Mitochondria 4 4 3 13 5 51 85 100 118 156 96 142 0 0 1 – 8 13 6 3 12 2 7 3
Nucleus 0 2 0 1 0 0 4 3 3 5 3 7 0 0 1 – 1 1 0 0 0 0 1 0
Nuclear Periphery 0 1 0 0 0 0 1 2 2 1 1 3 0 0 0 – 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 3 2 1 1 3 0 0 1 – 0 0 1 0 2 2 1 0
Vac/Vac Membrane 1 0 3 2 10 7 22 22 18 31 16 25 0 0 0 – 2 5 3 2 1 1 2 4
Unique Cell Count 277 210 215 143 175 201 317 324 242 307 218 284 58 60 73 278 356 284 213 202 100 183 179
Labelled Cell Count 279 213 218 155 184 239 374 387 312 397 265 362 58 60 76 306 397 284 213 202 100 183 179


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.6 5.7 5.9 5.7 4.8 4.8 5.1 5.0 4.2 4.4 4.2 6.3 6.3 6.1 4.8 5.0 6.3 5.9 7.0 7.2
Std Deviation (1e-4) 1.3 1.5 2.7 2.7 2.4 1.5 2.0 2.0 1.9 1.2 1.6 1.4 1.2 1.2 1.4 1.5 1.3 2.1 1.4 2.1 2.3
Intensity Change (Log2) – – – 0.07 -0.0 -0.24 -0.25 -0.15 -0.19 -0.42 -0.35 -0.44 0.15 0.15 0.1 -0.24 -0.19 0.15 0.07 0.3 0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm -0.1 -1.2 -2.9 -6.3 -6.4 -8.7 -9.0 -9.0 -9.8 1.4 0 0.8 – 0.5 -0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 2.8 3.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Mitochondria 3.5 0 0 0 8.5 11.5 0 0 0 0 0 0 – 0 1.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 0 2.4 0 3.0 2.9 3.1 4.0 3.0 3.6 0 0 0 – 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup116-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available