Standard name | |
---|---|
Human Ortholog | |
Description | Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 8 |
Bud | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 2 | 3 | 6 | 2 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 3 | 10 | 7 |
Bud Neck | 0 | 0 | 1 | 1 | 5 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 12 | 13 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 5 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 3 | 4 | 4 | 5 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 7 | 34 | 38 | 100 | 142 | 140 | 127 | 88 | 66 | 64 | 63 | 42 | 129 | 156 | 238 | 72 | 97 | 61 | 22 | 31 | 38 | 115 | 170 | 254 |
Endoplasmic Reticulum | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 9 | 8 | 0 | 0 | 1 | 1 | 1 | 3 |
Endosome | 0 | 3 | 0 | 2 | 10 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 39 | 7 | 15 | 14 | 2 | 5 | 1 | 2 | 3 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 2 | 2 | 1 |
Mitochondria | 6 | 0 | 0 | 7 | 8 | 56 | 132 | 87 | 145 | 114 | 151 | 86 | 1 | 1 | 0 | 7 | 7 | 3 | 5 | 4 | 19 | 10 | 20 | 19 |
Nucleus | 2 | 15 | 12 | 23 | 34 | 44 | 98 | 75 | 73 | 67 | 71 | 59 | 27 | 23 | 39 | 22 | 14 | 13 | 8 | 26 | 23 | 23 | 16 | 8 |
Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 6 | 7 | 5 | 5 | 8 | 1 | 5 | 2 | 3 | 17 | 26 | 29 | 1 | 0 | 0 | 4 | 7 | 8 | 4 | 11 | 10 |
Vac/Vac Membrane | 4 | 28 | 6 | 9 | 19 | 19 | 28 | 10 | 6 | 12 | 18 | 15 | 5 | 7 | 26 | 44 | 72 | 38 | 9 | 19 | 23 | 37 | 40 | 36 |
Unique Cell Count | 18 | 70 | 59 | 145 | 211 | 240 | 314 | 221 | 222 | 209 | 232 | 164 | 175 | 221 | 356 | 136 | 178 | 119 | 59 | 110 | 134 | 211 | 296 | 379 |
Labelled Cell Count | 20 | 82 | 59 | 150 | 226 | 272 | 394 | 275 | 298 | 270 | 315 | 213 | 184 | 231 | 385 | 157 | 216 | 139 | 59 | 110 | 134 | 211 | 296 | 379 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.0 | 5.0 | 4.5 | 4.2 | 5.0 | 3.9 | 3.7 | 4.0 | 3.3 | 3.6 | 3.5 | 3.8 | 5.0 | 5.2 | 5.1 | 6.4 | 6.5 | 5.9 | 5.0 | 5.0 | 5.3 |
Std Deviation (1e-4) | 0.5 | 1.6 | 0.9 | 0.9 | 2.2 | 1.4 | 1.6 | 1.5 | 0.8 | 1.1 | 1.3 | 1.3 | 1.2 | 1.4 | 1.3 | 1.4 | 2.5 | 1.8 | 1.6 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.09 | 0.16 | -0.2 | -0.26 | -0.15 | -0.42 | -0.32 | -0.37 | -0.23 | 0.15 | 0.22 | 0.2 | 0.52 | 0.55 | 0.41 | 0.17 | 0.16 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.6 | 0.4 | -0.9 | -3.4 | -3.4 | -4.9 | -4.7 | -5.4 | -5.3 | 1.4 | 0.9 | 0.4 | -1.5 | -1.3 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -0.8 | -0.8 | -0.4 | 1.7 | 2.0 | 1.9 | 1.7 | 1.6 | 2.2 | -0.9 | -2.0 | -2.0 | -0.7 | -2.7 | -1.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 0 | 0 | 0 | 0 |
Vacuole | 0 | -0.3 | 0 | -0.3 | 0 | 0 | 0 | 0 | -0.2 | 0 | 0 | 0 | 3.3 | 4.3 | 3.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.3069 | -0.7918 | -0.9664 | -0.7372 | 1.5868 | -0.6956 | 0.2165 | -0.4296 | -0.35 | -0.0338 | -0.4367 | -0.4089 | -1.4414 | -1.3649 | -1.5291 | -1.0392 | -2.0606 | -1.4222 |
Actin | 0.0332 | 0.0004 | 0.0017 | 0.0621 | 0.0289 | 0.0114 | 0.0494 | 0.0001 | 0.0137 | 0.0315 | 0.0043 | 0.0077 | 0.0301 | 0.0002 | 0.0021 | 0.1394 | 0.0001 | 0.0005 |
Bud | 0.0004 | 0.0002 | 0.0019 | 0.0005 | 0.0006 | 0.0046 | 0.0012 | 0.0001 | 0.0009 | 0.0005 | 0.0002 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0.0009 | 0.0019 | 0.0001 |
Bud Neck | 0.0045 | 0.001 | 0.0052 | 0.007 | 0.0022 | 0.0021 | 0.0072 | 0.0004 | 0.0021 | 0.0025 | 0.0005 | 0.005 | 0.0016 | 0.0009 | 0.0008 | 0.0045 | 0.0019 | 0.0037 |
Bud Periphery | 0.0005 | 0.0002 | 0.0006 | 0.001 | 0.0013 | 0.0011 | 0.0029 | 0.0002 | 0.0011 | 0.0009 | 0.0003 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0.0013 | 0.0008 | 0.0001 |
Bud Site | 0.0025 | 0.0011 | 0.0068 | 0.0046 | 0.0023 | 0.0002 | 0.0116 | 0.0009 | 0.0024 | 0.0019 | 0.0005 | 0.0003 | 0.0009 | 0.001 | 0.0008 | 0.0081 | 0.0012 | 0.0002 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.0707 | 0.0717 | 0.0817 | 0.0482 | 0.0693 | 0.0996 | 0.0686 | 0.0966 | 0.0661 | 0.0258 | 0.0562 | 0.073 | 0.1329 | 0.1338 | 0.0802 | 0.0888 | 0.1713 | 0.1966 |
Cytoplasmic Foci | 0.0266 | 0.0094 | 0.0053 | 0.0054 | 0.0183 | 0.0162 | 0.0415 | 0.005 | 0.0091 | 0.0215 | 0.0233 | 0.0039 | 0.0106 | 0.0163 | 0.0163 | 0.0085 | 0.0167 | 0.0196 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0006 | 0.0002 | 0.0006 | 0.0001 | 0.0006 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0015 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0062 | 0.0022 | 0.0032 | 0.0024 | 0.0168 | 0.0026 | 0.0052 | 0.0023 | 0.0017 | 0.003 | 0.0006 | 0.0017 | 0.0068 | 0.0036 | 0.0039 | 0.0025 | 0.0022 | 0.005 |
Endosome | 0.0203 | 0.0038 | 0.0038 | 0.0093 | 0.0694 | 0.0043 | 0.0397 | 0.0019 | 0.0124 | 0.0349 | 0.0142 | 0.0068 | 0.0155 | 0.0015 | 0.007 | 0.0133 | 0.0022 | 0.0031 |
Golgi | 0.0066 | 0.0005 | 0.0003 | 0.0065 | 0.0109 | 0.001 | 0.0141 | 0.0001 | 0.005 | 0.0193 | 0.0057 | 0.0025 | 0.004 | 0.0001 | 0.0008 | 0.0098 | 0.0001 | 0.0001 |
Lipid Particles | 0.017 | 0.002 | 0.0009 | 0.0056 | 0.0478 | 0.0018 | 0.0428 | 0.0004 | 0.005 | 0.0281 | 0.0255 | 0.0045 | 0.0055 | 0.0005 | 0.0065 | 0.0113 | 0.0007 | 0.0005 |
Mitochondria | 0.0029 | 0.0023 | 0.0015 | 0.0019 | 0.009 | 0.0012 | 0.0192 | 0.0005 | 0.0078 | 0.0098 | 0.0017 | 0.0044 | 0.0024 | 0.0004 | 0.0005 | 0.0138 | 0.0004 | 0.0007 |
None | 0.4137 | 0.4884 | 0.3899 | 0.6022 | 0.2908 | 0.4291 | 0.2484 | 0.4509 | 0.3188 | 0.5841 | 0.2448 | 0.309 | 0.2485 | 0.2995 | 0.2159 | 0.3082 | 0.3794 | 0.203 |
Nuclear Periphery | 0.055 | 0.0131 | 0.0316 | 0.0112 | 0.1784 | 0.019 | 0.0347 | 0.0146 | 0.023 | 0.0209 | 0.0079 | 0.0143 | 0.0458 | 0.0189 | 0.0173 | 0.0157 | 0.0096 | 0.0086 |
Nucleolus | 0.0091 | 0.0132 | 0.0097 | 0.0031 | 0.0078 | 0.0054 | 0.0144 | 0.0117 | 0.0212 | 0.0087 | 0.0035 | 0.0115 | 0.0074 | 0.0122 | 0.0105 | 0.0027 | 0.0097 | 0.0138 |
Nucleus | 0.2423 | 0.334 | 0.3546 | 0.2003 | 0.0583 | 0.3191 | 0.3065 | 0.3432 | 0.4518 | 0.1149 | 0.3655 | 0.4763 | 0.428 | 0.4499 | 0.5378 | 0.3474 | 0.3273 | 0.5122 |
Peroxisomes | 0.0068 | 0.0003 | 0.0087 | 0.002 | 0.0471 | 0.0009 | 0.0348 | 0.0018 | 0.0105 | 0.0305 | 0.1133 | 0.003 | 0.002 | 0.0002 | 0.001 | 0.0067 | 0.0002 | 0.0002 |
Punctate Nuclear | 0.0767 | 0.0541 | 0.0831 | 0.0244 | 0.1292 | 0.0783 | 0.0482 | 0.0672 | 0.0422 | 0.0572 | 0.1315 | 0.0732 | 0.0509 | 0.0587 | 0.0965 | 0.0129 | 0.0726 | 0.0298 |
Vacuole | 0.0028 | 0.0016 | 0.007 | 0.0009 | 0.0052 | 0.0011 | 0.0062 | 0.0017 | 0.0034 | 0.002 | 0.0004 | 0.0009 | 0.0045 | 0.0016 | 0.0015 | 0.0014 | 0.0011 | 0.0019 |
Vacuole Periphery | 0.0018 | 0.0005 | 0.0022 | 0.0005 | 0.0058 | 0.0005 | 0.0021 | 0.0002 | 0.0007 | 0.0015 | 0.0002 | 0.0007 | 0.0017 | 0.0004 | 0.0004 | 0.001 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.7227 | 9.3054 | 3.8361 | 9.1961 | 16.4955 | 13.8716 | 6.2725 | 5.6447 | 11.97 | 15.4343 |
Translational Efficiency | 2.542 | 1.4025 | 2.1601 | 1.4954 | 1.4503 | 3.0233 | 1.6335 | 1.7074 | 1.1621 | 1.6378 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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