Standard name
Description Mitochondrial protein of unknown function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.08 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.06 0 0 0 0.06 0.05 0.07 – 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.08 0.06 0.1 0 0.05 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Cytoplasm 0.1 0.08 0.38 0.33 0.4 0.29 0.23 0.32 0.14 – 0.14 0.17 0.32 0.48 0.51 0.3 0.26 0.25 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.05 0 0 0 0 0 – 0 0 0 0 0 0.08 0.05 0.07 0 0 0 0 0 0
Endosome 0.09 0 0 0 0.05 0 0 0 0 – 0 0 0.09 0.17 0.17 0.18 0.18 0.22 0.07 0.13 0.08 0.05 0.07 0.09
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0.76 0.81 0.26 0.49 0.27 0.7 0.7 0.46 0.86 – 0.84 0.79 0.61 0.29 0.29 0.17 0.13 0.17 0.74 0.69 0.72 0.62 0.63 0.6
Nucleus 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0.1 0.1 0.1 0.08 0 0 0 0 0 0.05 0
SpindlePole 0.05 0 0.15 0.11 0.19 0.07 0.11 0.14 0.06 – 0.09 0.1 0 0 0 0.15 0.18 0.16 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.11 0.05 0.06 0 0.05 0 0.05 0 – 0 0 0.08 0.12 0.06 0.1 0.21 0.22 0 0 0 0.08 0.06 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 3 14 2 3 0 0 1 2 – 1 2 1 0 0 1 2 2 4 4 6 5 8 9
Bud 10 7 10 4 4 2 19 10 22 – 20 12 4 4 3 1 4 1 0 0 1 1 4 3
Bud Neck 1 1 14 7 14 0 14 7 6 – 3 4 0 0 0 0 1 1 0 2 1 2 9 7
Bud Site 0 1 0 0 1 0 5 0 3 – 7 3 0 0 1 0 1 0 – – – – – –
Cell Periphery 4 4 2 1 2 2 2 1 1 – 1 1 1 3 2 3 9 0 0 1 0 1 1 0
Cytoplasm 33 18 67 40 59 48 70 68 46 – 43 41 64 98 112 34 34 19 4 2 7 4 5 2
Endoplasmic Reticulum 2 9 5 6 5 3 2 0 11 – 3 5 1 6 0 9 6 5 3 0 4 2 2 1
Endosome 28 7 3 4 7 3 6 4 2 – 0 3 18 35 37 21 24 17 24 36 21 8 22 20
Golgi 0 1 1 0 0 0 1 0 0 – 0 0 1 1 0 3 3 4 7 8 2 0 1 5
Mitochondria 241 192 45 59 39 114 215 98 283 – 251 195 124 60 64 20 17 13 265 197 205 99 187 136
Nucleus 8 1 2 1 3 2 10 11 6 – 4 1 0 0 1 3 2 3 0 0 0 1 0 1
Nuclear Periphery 0 1 0 0 0 1 0 0 1 – 1 2 0 0 0 0 0 1 4 2 0 0 2 2
Nucleolus 3 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 3 1 0 1 2 1
Peroxisomes 9 4 3 2 5 0 0 4 2 – 1 3 7 20 22 12 10 2 11 7 5 5 14 7
SpindlePole 15 7 26 13 28 11 35 30 20 – 26 26 4 1 7 17 23 12 5 6 5 4 11 4
Vac/Vac Membrane 35 27 9 7 6 8 7 10 10 – 7 6 17 24 14 12 27 17 14 8 12 12 18 15
Unique Cell Count 316 236 175 120 147 164 308 211 328 300 248 202 205 220 115 130 76 358 287 285 159 299 227
Labelled Cell Count 391 283 201 146 176 194 386 244 415 368 304 242 252 263 136 163 97 358 287 285 159 299 227


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.3 4.4 5.0 4.7 4.9 3.8 4.0 5.1 3.4 – 3.3 3.8 4.5 4.7 4.8 6.9 7.1 6.8 4.5 4.3 4.9
Std Deviation (1e-4) 0.5 0.6 1.0 2.5 1.3 1.2 1.1 3.5 1.1 – 1.3 1.1 1.7 1.7 2.2 1.5 2.1 1.8 1.9 1.2 1.9
Intensity Change (Log2) – – – -0.08 -0.02 -0.4 -0.33 0.02 -0.54 – -0.58 -0.4 -0.17 -0.1 -0.06 0.47 0.5 0.43 -0.17 -0.21 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 1.7 4.2 4.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1006 1.7477 1.6548 0.9758 1.5477 1.6444 0.216 0.2735 0.4242 0.1824 0.4737 0.1661 0.462 0.2892 0.1425 0.3841 1.1694 0.3441
Actin 0.0243 0.0168 0.0131 0.0038 0.0092 0.0159 0.0233 0.0402 0.0184 0.0337 0.0043 0.0192 0.0423 0.0115 0.0112 0.0486 0.0042 0.0377
Bud 0.0008 0.004 0.0021 0.0019 0.0015 0.0012 0.0025 0.0038 0.0039 0.0145 0.0021 0.0044 0.0236 0.0462 0.047 0.0175 0.0454 0.0344
Bud Neck 0.0042 0.0034 0.0143 0.0022 0.0014 0.0197 0.0017 0.003 0.002 0.0031 0.0012 0.0094 0.0052 0.0036 0.0127 0.0021 0.0018 0.026
Bud Periphery 0.0022 0.0103 0.0079 0.0125 0.0074 0.0047 0.005 0.0071 0.0078 0.0128 0.0069 0.0037 0.0301 0.0305 0.0378 0.0225 0.0308 0.0406
Bud Site 0.0082 0.0445 0.0174 0.0018 0.0015 0.0027 0.0105 0.0364 0.0124 0.0113 0.0039 0.0048 0.0585 0.2038 0.0978 0.0171 0.008 0.0118
Cell Periphery 0.0024 0.0037 0.0047 0.0106 0.002 0.0037 0.0019 0.0017 0.0011 0.0013 0.0009 0.001 0.0045 0.0038 0.0045 0.0015 0.002 0.0025
Cytoplasm 0.0332 0.0608 0.0365 0.0173 0.0342 0.0505 0.0229 0.0483 0.0647 0.0654 0.0024 0.0375 0.0431 0.0421 0.0479 0.0488 0.0335 0.1247
Cytoplasmic Foci 0.1248 0.1055 0.094 0.0689 0.03 0.1016 0.1434 0.1158 0.125 0.0831 0.0533 0.1104 0.1818 0.1149 0.1382 0.0863 0.1018 0.1889
Eisosomes 0.003 0.0018 0.0017 0.0003 0.0011 0.0011 0.0008 0.0007 0.0007 0.0005 0.0002 0.0003 0.002 0.0015 0.0008 0.0004 0.0003 0.0004
Endoplasmic Reticulum 0.0052 0.0047 0.0046 0.0017 0.0018 0.0052 0.0055 0.0024 0.0026 0.0024 0.0004 0.0059 0.0054 0.0019 0.0038 0.0036 0.0018 0.004
Endosome 0.1164 0.1015 0.0911 0.0572 0.1053 0.0978 0.1581 0.1594 0.1132 0.0921 0.1137 0.1503 0.0406 0.0751 0.0602 0.0431 0.0682 0.0291
Golgi 0.0403 0.0551 0.0437 0.0422 0.025 0.0532 0.0623 0.096 0.076 0.0549 0.0788 0.0819 0.0212 0.0193 0.0188 0.0194 0.0231 0.0117
Lipid Particles 0.0668 0.0339 0.0563 0.0401 0.0147 0.0508 0.1144 0.021 0.0125 0.0168 0.038 0.0208 0.0541 0.016 0.0217 0.0175 0.0846 0.0302
Mitochondria 0.3321 0.3554 0.4013 0.6216 0.5021 0.3823 0.2349 0.3212 0.4071 0.4261 0.6275 0.4537 0.157 0.1386 0.1785 0.3627 0.248 0.0677
None 0.1219 0.1019 0.0974 0.0303 0.1981 0.1129 0.0717 0.0876 0.0734 0.0733 0.0006 0.035 0.2407 0.1944 0.2002 0.1864 0.2463 0.2669
Nuclear Periphery 0.0042 0.001 0.0021 0.0006 0.0012 0.0034 0.0063 0.0005 0.0006 0.0007 0.0003 0.001 0.0043 0.0018 0.0041 0.0028 0.0024 0.0049
Nucleolus 0.0027 0.0011 0.002 0.0006 0.001 0.0019 0.0015 0.0033 0.0006 0.0023 0.0035 0.0014 0.0089 0.008 0.0107 0.0039 0.0038 0.0063
Nucleus 0.0035 0.0012 0.0016 0.001 0.003 0.0014 0.0017 0.0011 0.0012 0.002 0.001 0.002 0.0083 0.0166 0.0237 0.0052 0.0072 0.0315
Peroxisomes 0.087 0.082 0.0949 0.0729 0.0404 0.07 0.1025 0.0324 0.0601 0.0822 0.0442 0.0429 0.0398 0.0294 0.0369 0.0828 0.0563 0.0371
Punctate Nuclear 0.003 0.0007 0.0019 0.0006 0.0039 0.0025 0.0035 0.001 0.0012 0.0027 0.0003 0.0027 0.015 0.0263 0.0278 0.0158 0.0073 0.0315
Vacuole 0.0107 0.008 0.0081 0.0074 0.0107 0.0111 0.0177 0.0133 0.0103 0.0132 0.0117 0.0069 0.0113 0.0119 0.0121 0.009 0.0155 0.0096
Vacuole Periphery 0.0031 0.0028 0.0033 0.0044 0.0042 0.0062 0.008 0.0036 0.0053 0.0058 0.0049 0.0048 0.0024 0.0026 0.0037 0.0028 0.0073 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.114 4.023 5.6991 6.5242 4.2436 1.2267 5.8242 7.8619 6.3186 7.6781
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency –