Standard name | |
---|---|
Description | Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for assembly of alpha and beta subunits into F1 sector of mitochondrial F1F0 ATP synthase; human homolog ATPAF2 can complement yeast atp12 mutant; mutation of human homolog reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 2 |
Bud | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 4 | 5 | 10 | 7 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud Neck | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 11 | 9 | 6 | 3 | 4 | 5 | 6 | 8 | 4 | 5 | 7 | 2 | 3 | 46 | 42 | 58 | 0 | 0 | 5 | 0 | 1 | 1 |
Cytoplasm | 39 | 36 | 255 | 245 | 255 | 275 | 317 | 283 | 210 | 285 | 180 | 134 | 167 | 253 | 252 | 953 | 825 | 1045 | 61 | 37 | 212 | 74 | 65 | 97 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 49 | 45 | 80 | 0 | 0 | 0 | 0 | 2 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 1 | 1 | 4 | 0 | 1 | 0 | 2 | 1 | 1 | 0 | 2 | 0 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 9 | 11 | 0 | 0 | 3 | 0 | 2 | 0 |
Mitochondria | 2 | 3 | 1 | 10 | 14 | 89 | 153 | 114 | 147 | 126 | 143 | 42 | 2 | 0 | 0 | 9 | 5 | 14 | 0 | 1 | 14 | 4 | 5 | 9 |
Nucleus | 0 | 1 | 0 | 2 | 2 | 0 | 2 | 1 | 0 | 1 | 3 | 1 | 1 | 0 | 3 | 2 | 3 | 3 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 0 | 4 | 0 | 0 | 1 | 1 | 3 | 2 | 1 | 1 | 2 | 0 | 2 | 1 |
Vac/Vac Membrane | 2 | 1 | 1 | 2 | 3 | 11 | 13 | 5 | 7 | 10 | 2 | 5 | 1 | 1 | 1 | 9 | 12 | 23 | 0 | 1 | 0 | 0 | 0 | 2 |
Unique Cell Count | 43 | 41 | 258 | 254 | 267 | 336 | 406 | 359 | 308 | 377 | 262 | 178 | 170 | 257 | 257 | 965 | 842 | 1079 | 67 | 48 | 248 | 88 | 90 | 129 |
Labelled Cell Count | 45 | 41 | 275 | 270 | 282 | 382 | 496 | 416 | 380 | 450 | 342 | 200 | 179 | 258 | 263 | 1078 | 947 | 1238 | 67 | 48 | 248 | 88 | 90 | 129 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.9 | 5.1 | 5.8 | 5.8 | 5.5 | 4.4 | 4.2 | 4.5 | 4.1 | 4.2 | 3.9 | 4.4 | 6.2 | 6.3 | 6.6 | 5.7 | 6.0 | 5.9 | 6.3 | 6.3 | 6.4 |
Std Deviation (1e-4) | 1.0 | 1.0 | 1.6 | 2.5 | 2.4 | 1.6 | 1.6 | 1.3 | 1.4 | 0.9 | 1.4 | 1.0 | 1.2 | 1.5 | 2.0 | 1.3 | 1.6 | 1.6 | 2.2 | 2.6 | 2.0 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.07 | -0.38 | -0.47 | -0.36 | -0.49 | -0.47 | -0.58 | -0.38 | 0.1 | 0.13 | 0.2 | -0.03 | 0.07 | 0.04 | 0.13 | 0.14 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.4 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.1 | -2.5 | -2.2 | 0.3 | 0.5 | 0.7 |
Cytoplasm | -1.8 | -2.3 | -6.6 | -7.5 | -7.3 | -9.5 | -8.1 | -9.3 | -7.8 | -0.5 | -0.4 | -0.7 | -0.1 | -0.9 | -1.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 3.5 | 4.3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.8 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 2.5 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |