Standard name
Human Ortholog
Description Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for assembly of alpha and beta subunits into F1 sector of mitochondrial F1F0 ATP synthase; human homolog ATPAF2 can complement yeast atp12 mutant; mutation of human homolog reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.91 0.88 0.99 0.96 0.96 0.82 0.78 0.79 0.68 0.76 0.69 0.75 0.98 0.98 0.98 0.99 0.98 0.97 0.91 0.78 0.86 0.84 0.73 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0 0 0.05 0.26 0.38 0.32 0.48 0.33 0.55 0.24 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 3 2
Bud 1 0 1 0 0 0 3 4 5 10 7 1 0 0 0 0 0 0 0 0 0 0 2 1
Bud Neck 0 0 3 0 1 0 0 2 0 1 1 0 0 0 0 4 1 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 11 9 6 3 4 5 6 8 4 5 7 2 3 46 42 58 0 0 5 0 1 1
Cytoplasm 39 36 255 245 255 275 317 283 210 285 180 134 167 253 252 953 825 1045 61 37 212 74 65 97
Endoplasmic Reticulum 0 0 1 1 0 1 0 1 0 1 0 1 0 0 1 49 45 80 0 0 0 0 2 1
Endosome 0 0 0 0 0 2 2 1 2 1 1 4 0 1 0 2 1 1 0 2 0 1 2 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 1 2 3 9 11 0 0 3 0 2 0
Mitochondria 2 3 1 10 14 89 153 114 147 126 143 42 2 0 0 9 5 14 0 1 14 4 5 9
Nucleus 0 1 0 2 2 0 2 1 0 1 3 1 1 0 3 2 3 3 0 1 0 0 0 0
Nuclear Periphery 1 0 0 1 0 0 1 0 2 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 0 0 5 0 4 0 0 1 1 3 2 1 1 2 0 2 1
Vac/Vac Membrane 2 1 1 2 3 11 13 5 7 10 2 5 1 1 1 9 12 23 0 1 0 0 0 2
Unique Cell Count 43 41 258 254 267 336 406 359 308 377 262 178 170 257 257 965 842 1079 67 48 248 88 90 129
Labelled Cell Count 45 41 275 270 282 382 496 416 380 450 342 200 179 258 263 1078 947 1238 67 48 248 88 90 129


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.1 5.8 5.8 5.5 4.4 4.2 4.5 4.1 4.2 3.9 4.4 6.2 6.3 6.6 5.7 6.0 5.9 6.3 6.3 6.4
Std Deviation (1e-4) 1.0 1.0 1.6 2.5 2.4 1.6 1.6 1.3 1.4 0.9 1.4 1.0 1.2 1.5 2.0 1.3 1.6 1.6 2.2 2.6 2.0
Intensity Change (Log2) – – – 0.01 -0.07 -0.38 -0.47 -0.36 -0.49 -0.47 -0.58 -0.38 0.1 0.13 0.2 -0.03 0.07 0.04 0.13 0.14 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 -1.3 0 0 0 0 0 0 0 -0.1 -2.5 -2.2 0.3 0.5 0.7
Cytoplasm -1.8 -2.3 -6.6 -7.5 -7.3 -9.5 -8.1 -9.3 -7.8 -0.5 -0.4 -0.7 -0.1 -0.9 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.4 3.5 4.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 3.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.5 2.5 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.138 0.0044 0.0079 0.009 0.0023 0.0241 – – – – – –
Bud – – – – – – 0.0033 0.0536 0.0313 0.0107 0.009 0.0044 – – – – – –
Bud Neck – – – – – – 0.0827 0.0139 0.0172 0.0006 0.2283 0.0226 – – – – – –
Bud Periphery – – – – – – 0.0031 0.0173 0.0242 0.0036 0.0079 0.0026 – – – – – –
Bud Site – – – – – – 0.026 0.1546 0.0701 0.0015 0.0013 0.0082 – – – – – –
Cell Periphery – – – – – – 0.0009 0.003 0.0023 0.0002 0.0007 0.0004 – – – – – –
Cytoplasm – – – – – – 0.235 0.2179 0.2733 0.231 0.266 0.4846 – – – – – –
Cytoplasmic Foci – – – – – – 0.1374 0.0187 0.1265 0.0414 0.0156 0.026 – – – – – –
Eisosomes – – – – – – 0.0019 0.0003 0.0003 0.0002 0.0001 0.0001 – – – – – –
Endoplasmic Reticulum – – – – – – 0.0163 0.0033 0.008 0.0039 0.002 0.0026 – – – – – –
Endosome – – – – – – 0.0569 0.004 0.0569 0.1457 0.0017 0.0209 – – – – – –
Golgi – – – – – – 0.0368 0.0005 0.0139 0.0191 0.0002 0.001 – – – – – –
Lipid Particles – – – – – – 0.0462 0.0006 0.0102 0.1259 0.0009 0.0006 – – – – – –
Mitochondria – – – – – – 0.0184 0.0017 0.0044 0.0129 0.0013 0.0161 – – – – – –
None – – – – – – 0.0487 0.3918 0.2135 0.2132 0.4284 0.1921 – – – – – –
Nuclear Periphery – – – – – – 0.0063 0.0056 0.006 0.0178 0.003 0.0053 – – – – – –
Nucleolus – – – – – – 0.006 0.0052 0.0066 0.0013 0.003 0.011 – – – – – –
Nucleus – – – – – – 0.0033 0.0594 0.0317 0.1109 0.0173 0.0821 – – – – – –
Peroxisomes – – – – – – 0.0843 0.0007 0.0103 0.0223 0.0004 0.0014 – – – – – –
Punctate Nuclear – – – – – – 0.0443 0.0371 0.0773 0.0145 0.0061 0.0477 – – – – – –
Vacuole – – – – – – 0.0026 0.0061 0.0067 0.0107 0.0035 0.0353 – – – – – –
Vacuole Periphery – – – – – – 0.0015 0.0005 0.0013 0.0037 0.0009 0.011 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.2823 4.0343 10.4776 15.5082 13.1651 7.8932 16.5711 23.057 17.4246 20.6338
Translational Efficiency 1.3024 2.8604 1.2436 0.9741 0.839 1.9985 1.1958 0.8392 1.3264 1.1411

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for assembly of alpha and beta subunits into F1 sector of mitochondrial F1F0 ATP synthase; human homolog ATPAF2 can complement yeast atp12 mutant; mutation of human homolog reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
Localization
Cell Percentages mitochondrion (62%)
Cell Cycle Regulation No
Subcompartmental Group mito-3

Atp12

Atp12


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atp12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available