Standard name
Description Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for assembly of alpha and beta subunits into F1 sector of mitochondrial F1F0 ATP synthase; human homolog ATPAF2 can complement yeast atp12 mutant; mutation of human homolog reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.91 0.88 0.99 0.96 0.96 0.82 0.78 0.79 0.68 0.76 0.69 0.75 0.98 0.98 0.98 0.99 0.98 0.97 0.91 0.78 0.86 0.84 0.73 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.07 0 0 0.05 0.26 0.38 0.32 0.48 0.33 0.55 0.24 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 3 2
Bud 1 0 1 0 0 0 3 4 5 10 7 1 0 0 0 0 0 0 0 0 0 0 2 1
Bud Neck 0 0 3 0 1 0 0 2 0 1 1 0 0 0 0 4 1 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 11 9 6 3 4 5 6 8 4 5 7 2 3 46 42 58 0 0 5 0 1 1
Cytoplasm 39 36 255 245 255 275 317 283 210 285 180 134 167 253 252 953 825 1045 61 37 212 74 65 97
Endoplasmic Reticulum 0 0 1 1 0 1 0 1 0 1 0 1 0 0 1 49 45 80 0 0 0 0 2 1
Endosome 0 0 0 0 0 2 2 1 2 1 1 4 0 1 0 2 1 1 0 2 0 1 2 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 1 1 2 3 9 11 0 0 3 0 2 0
Mitochondria 2 3 1 10 14 89 153 114 147 126 143 42 2 0 0 9 5 14 0 1 14 4 5 9
Nucleus 0 1 0 2 2 0 2 1 0 1 3 1 1 0 3 2 3 3 0 1 0 0 0 0
Nuclear Periphery 1 0 0 1 0 0 1 0 2 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 1 0 0 5 0 4 0 0 1 1 3 2 1 1 2 0 2 1
Vac/Vac Membrane 2 1 1 2 3 11 13 5 7 10 2 5 1 1 1 9 12 23 0 1 0 0 0 2
Unique Cell Count 43 41 258 254 267 336 406 359 308 377 262 178 170 257 257 965 842 1079 67 48 248 88 90 129
Labelled Cell Count 45 41 275 270 282 382 496 416 380 450 342 200 179 258 263 1078 947 1238 67 48 248 88 90 129


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.9 5.1 5.8 5.8 5.5 4.4 4.2 4.5 4.1 4.2 3.9 4.4 6.2 6.3 6.6 5.7 6.0 5.9 6.3 6.3 6.4
Std Deviation (1e-4) 1.0 1.0 1.6 2.5 2.4 1.6 1.6 1.3 1.4 0.9 1.4 1.0 1.2 1.5 2.0 1.3 1.6 1.6 2.2 2.6 2.0
Intensity Change (Log2) – – – 0.01 -0.07 -0.38 -0.47 -0.36 -0.49 -0.47 -0.58 -0.38 0.1 0.13 0.2 -0.03 0.07 0.04 0.13 0.14 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.4 -1.3 0 0 0 0 0 0 0 -0.1 -2.5 -2.2 0.3 0.5 0.7
Cytoplasm -1.8 -2.3 -6.6 -7.5 -7.3 -9.5 -8.1 -9.3 -7.8 -0.5 -0.4 -0.7 -0.1 -0.9 -1.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.4 3.5 4.3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.8 3.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.5 2.5 0 0 0 0 0 0 0 0 0 0 0