Standard name
Human Ortholog
Description Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.55 0.43 0.54 0.6 0.42 0.46 0.42 0.32 0.4 0.41 0.44 0.4 0.29 0.36 0.32 0.28 0.23 0.26 0.63 0.59 0.53 0.45 0.49 0.5
Bud 0.13 0.1 0.1 0.13 0.12 0.12 0.08 0.08 0.11 0.09 0.1 0.08 0.05 0.05 0.07 0.07 0.08 0.06 0.05 0.08 0.05 0.06 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.19 0.19 0.14 0.07 0.09 0.08 0.07 0.07 0.05 0.05 0 0.05 0.19 0.36 0.25 0.11 0.1 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0.12 0.19 0.1 0.17 0.14 0.12 0.15 0.11 0.09 0.11 0.13 0.09 0 0.05 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.07 0.08 0.1 0.08 0 0.08
Golgi 0.43 0.48 0.6 0.68 0.73 0.65 0.69 0.66 0.68 0.73 0.62 0.64 0.62 0.41 0.68 0.77 0.8 0.74 0.14 0.14 0.2 0.23 0.31 0.29
Mitochondria 0.05 0 0 0 0 0.1 0.1 0 0.2 0.16 0.47 0.21 0.05 0 0.07 0.1 0.07 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 0.05 0 0 0 0.05 0 0.05 0 0.06 0.06 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 263 159 113 118 86 122 121 146 96 122 109 104 6 8 9 73 60 69 361 270 125 18 39 50
Bud 62 37 20 25 24 31 24 38 26 26 26 21 1 1 2 19 20 15 31 35 12 2 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 0 0 0
Bud Site 0 3 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 19 6 9 5 7 1 10 4 7 9 7 1 2 0 6 5 5 0 0 0 0 0 0
Cytoplasm 92 69 29 14 18 22 21 32 12 15 9 14 4 8 7 29 25 41 21 12 6 0 1 1
Endoplasmic Reticulum 58 69 21 33 29 31 42 50 21 32 33 24 0 1 1 8 12 17 7 7 3 0 0 1
Endosome 12 0 4 1 1 3 0 2 1 4 1 5 1 0 0 0 1 1 40 38 23 3 2 7
Golgi 205 176 126 134 149 170 196 304 165 220 154 167 13 9 19 199 211 200 79 61 46 9 25 28
Mitochondria 26 6 1 8 2 25 28 20 49 48 116 54 1 0 2 26 19 8 5 3 2 2 1 0
Nucleus 0 0 1 1 1 0 2 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 0 0 2 0 1 0 0 0 0 0 0 2 1 0 0 0 0
Nucleolus 8 5 2 2 1 6 7 20 8 12 7 16 0 0 0 7 3 4 0 0 0 0 0 0
Peroxisomes 3 0 0 3 0 1 3 0 0 0 0 1 0 0 0 0 0 0 6 5 4 0 3 3
SpindlePole 2 0 0 2 0 1 1 5 3 1 0 0 0 0 0 8 3 2 5 6 2 0 0 0
Vac/Vac Membrane 9 11 12 4 5 7 3 24 6 11 4 14 0 1 0 15 15 10 2 7 2 1 0 0
Unique Cell Count 476 368 210 196 204 263 286 458 243 301 248 259 21 22 28 260 263 270 571 455 236 41 81 100
Labelled Cell Count 751 554 335 354 322 427 450 652 391 502 469 430 28 30 40 390 374 372 571 455 236 41 81 100


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.2 26.2 20.1 18.7 22.1 22.6 21.6 22.3 20.5 21.7 20.7 21.9 30.0 25.5 25.4 39.2 42.0 38.4 – 27.4 26.9
Std Deviation (1e-4) 4.5 6.3 4.6 4.0 5.4 5.9 5.4 5.6 5.8 6.7 6.1 6.8 10.8 9.3 8.3 13.9 14.2 13.1 – 8.8 12.9
Intensity Change (Log2) – – – -0.1 0.14 0.17 0.1 0.15 0.03 0.11 0.04 0.12 0.58 0.34 0.34 0.96 1.06 0.93 – 0.45 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 -2.4 -1.6 -2.5 -5.4 -3.0 -3.0 -2.1 -2.9 -2.2 -1.6 -2.2 -5.7 -7.0 -6.3
Bud 1.0 0.7 0.8 -0.4 -0.5 0.4 -0.3 0.3 -0.5 0 0 0 -0.9 -0.7 -1.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 -0.3 -0.1 0 -0.5 0 -0.4 0.5 -0.1 0 0 0 -0.4 0 0
Cytoplasm -2.2 -1.6 -1.9 -2.4 -2.8 -3.3 -3.5 -3.9 -3.1 0 0 0 -0.9 -1.5 0.4
Endoplasmic Reticulum 2.0 1.3 0.6 1.5 0.4 -0.5 0.2 1.1 -0.3 0 0 0 -3.1 -2.3 -1.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.8 2.8 1.0 2.0 1.6 1.7 3.1 0.5 1.0 0.2 -1.7 0.8 3.9 4.8 3.3
Mitochondria 0 0 4.3 4.4 2.7 6.7 5.8 11.3 6.8 0 0 0 4.4 3.6 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.3 0 2.1 0 2.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -1.7 -1.7 -3.0 -0.3 -1.8 -1.1 -2.4 -0.1 0 0 0 0 0 -1.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.4491 15.3667 16.1763 14.531 13.8738 15.0051 24.6628 20.8958 23.5461 22.862 22.5148 21.4372 20.6307 18.2925 18.1602 16.8367 18.6626 19.1425
Actin 0.6109 0.6079 0.8188 0.8083 0.7589 0.6826 0.6047 0.6121 0.7864 0.6931 0.6631 0.6048 0.7046 0.6649 0.8783 0.7914 0.8391 0.7315
Bud 0.0021 0.0074 0.0225 0.0169 0.0038 0.0019 0.002 0.004 0.0098 0.0108 0.0039 0.0007 0.0027 0.0064 0.0245 0.0248 0.0155 0.0009
Bud Neck 0.0021 0.0016 0.0015 0.0021 0.001 0.0038 0.0011 0.0014 0.0008 0.0009 0.0013 0.0031 0.0012 0.0036 0.0007 0.0041 0.0012 0.0068
Bud Periphery 0.0018 0.0058 0.0143 0.0091 0.0024 0.0019 0.0019 0.0021 0.0104 0.0271 0.0031 0.0006 0.002 0.0027 0.0227 0.0298 0.0218 0.0007
Bud Site 0.0043 0.0285 0.0161 0.0053 0.0015 0.0013 0.0033 0.0352 0.0115 0.0016 0.0022 0.0006 0.0054 0.0379 0.0082 0.004 0.0024 0.0006
Cell Periphery 0.0002 0.0004 0.0003 0.0009 0.0001 0.0002 0.0008 0.0004 0.0004 0.0003 0.0002 0.0002 0.0002 0.0004 0.0003 0.0004 0.0005 0.0002
Cytoplasm 0.0077 0.0727 0.0194 0.0547 0.0088 0.0068 0.0148 0.0376 0.0174 0.1384 0.0187 0.0059 0.0087 0.07 0.0026 0.0549 0.0328 0.0043
Cytoplasmic Foci 0.0438 0.0777 0.025 0.0206 0.021 0.0274 0.0321 0.0551 0.0251 0.0083 0.0275 0.0239 0.028 0.0655 0.0091 0.0128 0.0085 0.0262
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0002 0.0003 0.0005 0.0003 0.0002 0.0002 0.0003 0.0005 0.0015 0.0002 0.0002 0.0001 0.0017 0.0008
Endoplasmic Reticulum 0.0029 0.0011 0.0007 0.0004 0.0004 0.0008 0.001 0.001 0.0017 0.0014 0.0022 0.001 0.0005 0.0004 0.0001 0.0019 0.0011 0.0004
Endosome 0.047 0.0402 0.0162 0.0114 0.0227 0.0304 0.0258 0.0208 0.0122 0.0043 0.022 0.0217 0.0203 0.0171 0.0029 0.0085 0.0125 0.0181
Golgi 0.2271 0.1224 0.0515 0.0605 0.096 0.2282 0.2969 0.2065 0.1098 0.1095 0.2429 0.3267 0.2033 0.1027 0.043 0.0609 0.0542 0.2007
Lipid Particles 0.0099 0.0043 0.0011 0.0006 0.0288 0.0042 0.0054 0.0031 0.0028 0.0007 0.001 0.0009 0.0066 0.0013 0.0012 0.002 0.004 0.0015
Mitochondria 0.0124 0.0021 0.0024 0.0016 0.0048 0.0047 0.0046 0.0037 0.0018 0.0008 0.0027 0.0042 0.0042 0.0026 0.0008 0.0009 0.0016 0.0025
None 0.0026 0.0004 0.0008 0.0002 0.0014 0.0001 0.0001 0.0002 0.0002 0.0002 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0001
Nuclear Periphery 0.0074 0.0006 0.0007 0.0003 0.0056 0.0006 0.0002 0.0002 0.0002 0.0002 0.0006 0.0001 0.0001 0.0002 0.0001 0.0002 0.0004 0.0001
Nucleolus 0.0004 0.0001 0.0001 0.0001 0.0015 0 0.0001 0 0.0001 0 0 0 0 0.0001 0 0 0 0
Nucleus 0.0015 0.0004 0.0007 0.0008 0.0015 0.0001 0.0001 0.0002 0.0003 0.0001 0.0002 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0
Peroxisomes 0.0095 0.0232 0.0053 0.0031 0.006 0.0034 0.0033 0.0152 0.0079 0.0015 0.007 0.0044 0.0096 0.0219 0.0044 0.002 0.0014 0.0038
Punctate Nuclear 0.0027 0.001 0.001 0.0003 0.0319 0.0003 0.0001 0.0002 0.0002 0.0001 0.0003 0.0001 0.0001 0.0006 0.0001 0.0002 0.0002 0.0001
Vacuole 0.0017 0.0013 0.0009 0.0023 0.0006 0.0005 0.0005 0.0004 0.0007 0.0001 0.0004 0.0002 0.0002 0.0004 0.0004 0.0005 0.0003 0.0004
Vacuole Periphery 0.0017 0.0005 0.0004 0.0004 0.0009 0.0004 0.0005 0.0003 0.0002 0.0001 0.0003 0.0003 0.0003 0.0002 0.0001 0.0001 0.0003 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 72.2467 54.9766 60.9945 81.4023 61.5442 73.4002 62.1978 73.7598 86.3206 83.3591
Translational Efficiency 1.2573 1.2255 1.1263 1.2764 1.4092 1.2828 1.244 1.0784 1.2141 1.1624

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
Localization
Cell Percentages actin (75%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Aip1

Aip1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aip1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available