Standard name
Description Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; protein increases in abundance and relocalizes from cytoplasm to plasma membrane upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.55 0.43 0.54 0.6 0.42 0.46 0.42 0.32 0.4 0.41 0.44 0.4 0.29 0.36 0.32 0.28 0.23 0.26 0.63 0.59 0.53 0.45 0.49 0.5
Bud 0.13 0.1 0.1 0.13 0.12 0.12 0.08 0.08 0.11 0.09 0.1 0.08 0.05 0.05 0.07 0.07 0.08 0.06 0.05 0.08 0.05 0.06 0.05 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0.05 0.09 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.19 0.19 0.14 0.07 0.09 0.08 0.07 0.07 0.05 0.05 0 0.05 0.19 0.36 0.25 0.11 0.1 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0.12 0.19 0.1 0.17 0.14 0.12 0.15 0.11 0.09 0.11 0.13 0.09 0 0.05 0 0 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.07 0.08 0.1 0.08 0 0.08
Golgi 0.43 0.48 0.6 0.68 0.73 0.65 0.69 0.66 0.68 0.73 0.62 0.64 0.62 0.41 0.68 0.77 0.8 0.74 0.14 0.14 0.2 0.23 0.31 0.29
Mitochondria 0.05 0 0 0 0 0.1 0.1 0 0.2 0.16 0.47 0.21 0.05 0 0.07 0.1 0.07 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.06 0 0 0 0 0.05 0 0 0 0.05 0 0.05 0 0.06 0.06 0 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 263 159 113 118 86 122 121 146 96 122 109 104 6 8 9 73 60 69 361 270 125 18 39 50
Bud 62 37 20 25 24 31 24 38 26 26 26 21 1 1 2 19 20 15 31 35 12 2 4 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 1 1 0 0 0
Bud Site 0 3 0 0 0 1 0 0 0 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 11 19 6 9 5 7 1 10 4 7 9 7 1 2 0 6 5 5 0 0 0 0 0 0
Cytoplasm 92 69 29 14 18 22 21 32 12 15 9 14 4 8 7 29 25 41 21 12 6 0 1 1
Endoplasmic Reticulum 58 69 21 33 29 31 42 50 21 32 33 24 0 1 1 8 12 17 7 7 3 0 0 1
Endosome 12 0 4 1 1 3 0 2 1 4 1 5 1 0 0 0 1 1 40 38 23 3 2 7
Golgi 205 176 126 134 149 170 196 304 165 220 154 167 13 9 19 199 211 200 79 61 46 9 25 28
Mitochondria 26 6 1 8 2 25 28 20 49 48 116 54 1 0 2 26 19 8 5 3 2 2 1 0
Nucleus 0 0 1 1 1 0 2 1 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 0 0 2 0 1 0 0 0 0 0 0 2 1 0 0 0 0
Nucleolus 8 5 2 2 1 6 7 20 8 12 7 16 0 0 0 7 3 4 0 0 0 0 0 0
Peroxisomes 3 0 0 3 0 1 3 0 0 0 0 1 0 0 0 0 0 0 6 5 4 0 3 3
SpindlePole 2 0 0 2 0 1 1 5 3 1 0 0 0 0 0 8 3 2 5 6 2 0 0 0
Vac/Vac Membrane 9 11 12 4 5 7 3 24 6 11 4 14 0 1 0 15 15 10 2 7 2 1 0 0
Unique Cell Count 476 368 210 196 204 263 286 458 243 301 248 259 21 22 28 260 263 270 571 455 236 41 81 100
Labelled Cell Count 751 554 335 354 322 427 450 652 391 502 469 430 28 30 40 390 374 372 571 455 236 41 81 100


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.2 26.2 20.1 18.7 22.1 22.6 21.6 22.3 20.5 21.7 20.7 21.9 30.0 25.5 25.4 39.2 42.0 38.4 – 27.4 26.9
Std Deviation (1e-4) 4.5 6.3 4.6 4.0 5.4 5.9 5.4 5.6 5.8 6.7 6.1 6.8 10.8 9.3 8.3 13.9 14.2 13.1 – 8.8 12.9
Intensity Change (Log2) – – – -0.1 0.14 0.17 0.1 0.15 0.03 0.11 0.04 0.12 0.58 0.34 0.34 0.96 1.06 0.93 – 0.45 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.3 -2.4 -1.6 -2.5 -5.4 -3.0 -3.0 -2.1 -2.9 -2.2 -1.6 -2.2 -5.7 -7.0 -6.3
Bud 1.0 0.7 0.8 -0.4 -0.5 0.4 -0.3 0.3 -0.5 0 0 0 -0.9 -0.7 -1.7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 -0.3 -0.1 0 -0.5 0 -0.4 0.5 -0.1 0 0 0 -0.4 0 0
Cytoplasm -2.2 -1.6 -1.9 -2.4 -2.8 -3.3 -3.5 -3.9 -3.1 0 0 0 -0.9 -1.5 0.4
Endoplasmic Reticulum 2.0 1.3 0.6 1.5 0.4 -0.5 0.2 1.1 -0.3 0 0 0 -3.1 -2.3 -1.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1.8 2.8 1.0 2.0 1.6 1.7 3.1 0.5 1.0 0.2 -1.7 0.8 3.9 4.8 3.3
Mitochondria 0 0 4.3 4.4 2.7 6.7 5.8 11.3 6.8 0 0 0 4.4 3.6 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 2.3 0 2.1 0 2.9 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -1.7 -1.7 -3.0 -0.3 -1.8 -1.1 -2.4 -0.1 0 0 0 0 0 -1.0