Standard name
Description Acetolactate synthase; catalyses the first common step in isoleucine and valine biosynthesis and is the target of several classes of inhibitors, localizes to the mitochondria; expression of the gene is under general amino acid control
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.13 0.08 0.1 0.1
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Cytoplasm 0.05 0.14 0.11 0.1 0.1 0.08 0.1 – 0.07 0 0 0 0.09 0.07 0.14 0.25 0.21 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.09 0.11 0.15 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.11 0.18 0.05 0.08 0.08 0.14 – 0.1 0.18 0 0.12 0.1 0.11 0.12 0.18 0.15 0.09 0.24 0.24 0.25 0.4 0.41 0.4
Mitochondria 0.77 0.59 0.46 0.73 0.54 0.73 0.58 – 0.71 0.68 0.86 0.77 0.65 0.65 0.44 0.33 0.32 0.18 0.38 0.28 0.32 0.23 0.23 0.25
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.45 0.29 0.28 0.25 0.39 0.28 0.31 – 0.24 0.26 0.18 0.2 0.23 0.27 0.31 0.24 0.3 0.3 0.1 0.06 0.06 0.07 0.06 0.05
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0.05
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.11 0.22 0.09 0.13 0.12 0.18 – 0.15 0.13 0.06 0.09 0.17 0.17 0.16 0.06 0.1 0.22 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 1 1 2 0 4 – 5 9 7 8 2 3 0 1 0 0 6 16 36 14 40 37
Bud 0 0 0 0 0 0 0 – 1 0 2 2 0 0 0 0 0 0 0 2 1 2 7 7
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 2 3 0 2 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 3 1 0 2 3 – 1 7 0 7 1 1 0 5 6 6 0 0 0 0 0 0
Cytoplasm 7 18 24 22 27 28 50 – 26 19 6 24 21 24 46 29 26 23 0 0 1 1 2 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 1 0 2 0 0 0 10 13 17 1 6 0 2 5 6
Endosome 0 0 0 0 0 1 0 – 0 1 0 0 0 0 0 0 0 0 2 3 7 2 6 4
Golgi 0 14 40 12 22 28 68 – 40 89 15 71 24 37 38 21 18 10 26 37 70 72 170 143
Mitochondria 109 75 103 163 149 262 281 – 280 342 325 447 157 211 142 38 39 21 42 42 90 41 97 91
Nucleus 2 3 9 6 7 6 8 – 8 2 5 7 2 3 11 9 6 7 1 4 6 3 7 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 1 0 1 0 0 0 0 0 0 0 1 0 0 1 0
Nucleolus 63 37 62 55 107 99 151 – 94 129 70 119 56 87 98 27 36 35 10 9 15 13 24 17
Peroxisomes 0 0 1 2 2 3 2 – 3 0 4 0 2 1 2 0 0 0 3 4 24 6 12 17
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 4 2 0 1 2
Vac/Vac Membrane 25 14 50 21 35 45 86 – 58 65 24 51 42 56 52 7 12 26 2 5 1 7 9 6
Unique Cell Count 141 127 223 223 275 360 486 393 502 380 583 243 327 320 114 122 116 112 155 285 181 419 361
Labelled Cell Count 207 165 293 283 351 474 653 516 665 458 739 307 423 390 147 156 145 112 155 285 181 419 361


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 116.1 116.6 110.1 102.0 99.8 93.6 89.9 – 78.7 79.1 66.0 68.2 107.1 111.2 108.0 162.3 158.6 157.9 93.7 102.9 106.3
Std Deviation (1e-4) 30.8 48.5 38.1 35.7 31.6 36.5 36.0 – 35.5 31.0 25.4 25.9 40.5 35.3 38.8 59.7 55.7 70.6 40.2 42.0 41.9
Intensity Change (Log2) – – – -0.11 -0.14 -0.23 -0.29 – -0.48 -0.48 -0.74 -0.69 -0.04 0.01 -0.03 0.56 0.53 0.52 -0.23 -0.1 -0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.3 -0.3 -1.2 -0.2 – -1.8 -3.7 -5.0 -3.6 -0.8 -1.4 1.2 3.5 2.7 2.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 5.9
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi -4.1 -3.3 -3.7 -1.4 – -2.8 -0.1 -5.8 -2.1 -2.5 -2.2 -2.0 0.1 -0.8 -2.3
Mitochondria 5.8 1.8 6.4 2.9 – 6.2 5.6 10.3 8.3 4.0 4.3 -0.4 -2.3 -2.6 -5.1
Nucleus -0.8 -0.9 -1.8 -1.9 – -1.5 0 -2.1 0 -2.3 0 -0.4 1.5 0.4 0.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.8 2.6 -0.1 0.9 – -1.1 -0.6 -2.7 -2.2 -1.2 -0.3 0.7 -0.8 0.3 0.5
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole -3.8 -2.9 -3.2 -1.5 – -2.4 -3.2 -5.8 -5.2 -1.4 -1.5 -1.8 -3.8 -2.9 0