Standard name | |
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Description | Specific chaperone for ribosomal protein Rpl3p; binds to nascent Rpl3p during translation; essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 2 |
Bud | 0 | 0 | 0 | 3 | 1 | 0 | 2 | 6 | 4 | 6 | 6 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 11 | 6 | 2 | 10 | 9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 10 | 5 | 15 | 5 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 27 | 17 | 10 | 5 | 14 | 9 | 11 | 17 | 15 | 12 | 9 | 21 | 67 | 100 | 45 | 23 | 9 | 8 | 11 | 21 | 6 | 13 | 10 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 12 | 6 | 1 | 0 | 0 | 1 | 0 | 2 | 2 |
Endosome | 0 | 6 | 22 | 4 | 17 | 29 | 45 | 87 | 25 | 27 | 29 | 23 | 25 | 96 | 98 | 25 | 4 | 5 | 3 | 10 | 17 | 12 | 41 | 60 |
Golgi | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 10 | 32 | 13 | 0 | 1 | 1 | 0 | 3 | 1 |
Mitochondria | 1 | 1 | 2 | 21 | 9 | 49 | 43 | 54 | 58 | 81 | 73 | 97 | 3 | 0 | 0 | 24 | 9 | 4 | 0 | 2 | 0 | 0 | 0 | 2 |
Nucleus | 26 | 73 | 8 | 11 | 22 | 15 | 26 | 35 | 16 | 29 | 36 | 45 | 6 | 0 | 0 | 31 | 28 | 14 | 55 | 140 | 19 | 14 | 8 | 7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 3 | 5 | 1 | 2 | 6 |
Nucleolus | 27 | 34 | 34 | 90 | 100 | 146 | 135 | 267 | 101 | 129 | 106 | 124 | 126 | 83 | 64 | 155 | 96 | 81 | 48 | 87 | 33 | 50 | 39 | 33 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 2 | 10 | 8 | 24 | 46 | 71 | 12 | 18 | 18 | 20 | 10 | 8 | 2 | 3 | 1 | 1 | 8 | 11 | 26 | 19 | 45 | 37 |
Vac/Vac Membrane | 72 | 142 | 60 | 0 | 18 | 21 | 28 | 35 | 18 | 39 | 24 | 23 | 20 | 64 | 30 | 103 | 43 | 29 | 12 | 39 | 10 | 16 | 14 | 15 |
Unique Cell Count | 123 | 257 | 118 | 109 | 148 | 215 | 223 | 406 | 182 | 257 | 218 | 251 | 170 | 247 | 244 | 336 | 205 | 138 | 149 | 323 | 150 | 128 | 192 | 201 |
Labelled Cell Count | 134 | 286 | 146 | 149 | 181 | 301 | 338 | 580 | 257 | 360 | 310 | 361 | 211 | 323 | 294 | 413 | 242 | 162 | 149 | 323 | 150 | 128 | 192 | 201 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.9 | 16.3 | 14.2 | 13.1 | 12.3 | 9.9 | 8.4 | 9.0 | 8.2 | 7.8 | 7.7 | 8.0 | 17.7 | 15.8 | 15.3 | 19.3 | 26.6 | 27.6 | 17.1 | 15.7 | 16.4 |
Std Deviation (1e-4) | 3.0 | 3.5 | 2.8 | 4.3 | 2.7 | 2.5 | 2.1 | 2.0 | 2.1 | 1.9 | 1.8 | 1.9 | 4.2 | 3.1 | 2.6 | 5.5 | 7.0 | 6.6 | 3.7 | 3.6 | 3.5 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.21 | -0.52 | -0.76 | -0.67 | -0.8 | -0.87 | -0.89 | -0.83 | 0.31 | 0.15 | 0.11 | 0.44 | 0.9 | 0.96 | 0.27 | 0.15 | 0.2 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -3.2 | -2.4 | -3.4 | -5.0 | -1.4 | -2.8 | -2.8 | -3.8 | -0.5 | 2.7 | 5.1 | -0.3 | -0.8 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.5 | -1.6 | -1.2 | 0.3 | 0.7 | -1.1 | -2.2 | -1.3 | -2.6 | -0.9 | 3.9 | 4.1 | -3.4 | -5.3 | -3.9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 3.4 |
Mitochondria | 4.4 | 0 | 5.1 | 4.6 | 3.6 | 6.4 | 6.5 | 6.7 | 7.5 | 0 | 0 | 0 | 2.2 | 0 | 0 |
Nucleus | 0.9 | 2.1 | 0.1 | 1.4 | 0.6 | 0.6 | 1.4 | 2.5 | 2.8 | -1.3 | 0 | 0 | 0.8 | 1.9 | 1.0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 8.1 | 6.3 | 6.8 | 5.6 | 7.1 | 4.5 | 3.9 | 3.5 | 3.7 | 7.6 | 0.9 | -0.5 | 3.3 | 3.2 | 4.8 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 2.5 | 0 | 3.1 | 4.8 | 4.4 | 2.0 | 2.1 | 2.4 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -8.7 | -6.9 | -8.4 | -7.7 | -10.5 | -7.9 | -7.3 | -8.1 | -8.9 | -7.3 | -4.7 | -8.0 | -3.9 | -5.5 | -5.0 |