Standard name
Description Specific chaperone for ribosomal protein Rpl3p; binds to nascent Rpl3p during translation; essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.11 0.14 0.09 0 0.07 0 0 0.09 0.06 0.06 0 0.12 0.27 0.41 0.13 0.11 0.07 0.06 0 0.14 0.05 0.07 0.05
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0.19 0 0.11 0.13 0.2 0.21 0.14 0.11 0.13 0.09 0.15 0.39 0.4 0.07 0 0 0 0 0.12 0.1 0.22 0.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.09 0 0 0 0 0 0
Mitochondria 0 0 0 0.19 0.06 0.23 0.19 0.13 0.32 0.32 0.33 0.39 0 0 0 0.07 0 0 0 0 0 0 0 0
Nucleus 0.21 0.28 0.07 0.1 0.15 0.07 0.12 0.09 0.09 0.11 0.17 0.18 0 0 0 0.09 0.14 0.1 0.37 0.43 0.13 0.11 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.13 0.29 0.83 0.68 0.68 0.61 0.66 0.55 0.5 0.49 0.49 0.74 0.34 0.26 0.46 0.47 0.59 0.33 0.27 0.23 0.4 0.21 0.17
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.09 0.05 0.11 0.21 0.17 0.07 0.07 0.08 0.08 0.06 0 0 0 0 0 0.06 0 0.18 0.15 0.24 0.19
Vac/Vac Membrane 0.59 0.55 0.51 0 0.12 0.1 0.13 0.09 0.1 0.15 0.11 0.09 0.12 0.26 0.12 0.31 0.21 0.21 0.08 0.12 0.07 0.13 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 5 0 2 0 0 0 0 2 2
Bud 0 0 0 3 1 0 2 6 4 6 6 5 0 0 0 0 0 1 5 11 6 2 10 9
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 1 2
Bud Site 0 0 0 0 0 1 2 10 5 15 5 13 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 8 27 17 10 5 14 9 11 17 15 12 9 21 67 100 45 23 9 8 11 21 6 13 10
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 1 0 0 1 0 12 6 1 0 0 1 0 2 2
Endosome 0 6 22 4 17 29 45 87 25 27 29 23 25 96 98 25 4 5 3 10 17 12 41 60
Golgi 0 3 0 0 0 0 0 0 0 0 0 0 0 4 0 10 32 13 0 1 1 0 3 1
Mitochondria 1 1 2 21 9 49 43 54 58 81 73 97 3 0 0 24 9 4 0 2 0 0 0 2
Nucleus 26 73 8 11 22 15 26 35 16 29 36 45 6 0 0 31 28 14 55 140 19 14 8 7
Nuclear Periphery 0 0 0 0 1 1 0 1 1 0 0 1 0 0 0 0 0 1 4 3 5 1 2 6
Nucleolus 27 34 34 90 100 146 135 267 101 129 106 124 126 83 64 155 96 81 48 87 33 50 39 33
Peroxisomes 0 0 0 0 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 2 10 8 24 46 71 12 18 18 20 10 8 2 3 1 1 8 11 26 19 45 37
Vac/Vac Membrane 72 142 60 0 18 21 28 35 18 39 24 23 20 64 30 103 43 29 12 39 10 16 14 15
Unique Cell Count 123 257 118 109 148 215 223 406 182 257 218 251 170 247 244 336 205 138 149 323 150 128 192 201
Labelled Cell Count 134 286 146 149 181 301 338 580 257 360 310 361 211 323 294 413 242 162 149 323 150 128 192 201


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 14.9 16.3 14.2 13.1 12.3 9.9 8.4 9.0 8.2 7.8 7.7 8.0 17.7 15.8 15.3 19.3 26.6 27.6 17.1 15.7 16.4
Std Deviation (1e-4) 3.0 3.5 2.8 4.3 2.7 2.5 2.1 2.0 2.1 1.9 1.8 1.9 4.2 3.1 2.6 5.5 7.0 6.6 3.7 3.6 3.5
Intensity Change (Log2) – – – -0.11 -0.21 -0.52 -0.76 -0.67 -0.8 -0.87 -0.89 -0.83 0.31 0.15 0.11 0.44 0.9 0.96 0.27 0.15 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -3.2 -2.4 -3.4 -5.0 -1.4 -2.8 -2.8 -3.8 -0.5 2.7 5.1 -0.3 -0.8 -2.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.5 -1.6 -1.2 0.3 0.7 -1.1 -2.2 -1.3 -2.6 -0.9 3.9 4.1 -3.4 -5.3 -3.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 4.5 3.4
Mitochondria 4.4 0 5.1 4.6 3.6 6.4 6.5 6.7 7.5 0 0 0 2.2 0 0
Nucleus 0.9 2.1 0.1 1.4 0.6 0.6 1.4 2.5 2.8 -1.3 0 0 0.8 1.9 1.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 8.1 6.3 6.8 5.6 7.1 4.5 3.9 3.5 3.7 7.6 0.9 -0.5 3.3 3.2 4.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2.5 0 3.1 4.8 4.4 2.0 2.1 2.4 2.4 0 0 0 0 0 0
Vacuole -8.7 -6.9 -8.4 -7.7 -10.5 -7.9 -7.3 -8.1 -8.9 -7.3 -4.7 -8.0 -3.9 -5.5 -5.0