Standard name | |
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Description | Protein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein) |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 2 | 3 | 7 | 6 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 8 |
Bud Neck | 0 | 0 | 1 | 0 | 1 | 0 | 1 | – | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 1 | 1 | 1 | 3 | – | 4 | 4 | 3 | 1 | 0 | 0 | 2 | 4 | 10 | 8 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 287 | 148 | 97 | 216 | 342 | 266 | 426 | – | 258 | 334 | 258 | 272 | 170 | 391 | 459 | 298 | 200 | 259 | 280 | 145 | 105 | 229 | 277 | 333 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 17 | 11 | 15 | 0 | 0 | 0 | 1 | 4 | 1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 2 | 0 | 1 | 4 | 1 | 1 | 1 | 1 | 6 |
Golgi | 0 | 1 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 3 | 3 | 0 | 0 | 2 | 2 |
Mitochondria | 1 | 4 | 0 | 2 | 4 | 39 | 25 | – | 75 | 122 | 158 | 144 | 1 | 4 | 1 | 4 | 2 | 2 | 2 | 1 | 1 | 1 | 5 | 6 |
Nucleus | 0 | 0 | 3 | 0 | 0 | 3 | 2 | – | 3 | 4 | 4 | 6 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 1 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 3 | – | 3 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 4 |
Vac/Vac Membrane | 8 | 0 | 0 | 0 | 10 | 4 | 19 | – | 12 | 17 | 15 | 8 | 1 | 0 | 1 | 5 | 3 | 5 | 1 | 1 | 0 | 1 | 1 | 4 |
Unique Cell Count | 297 | 151 | 99 | 217 | 352 | 286 | 452 | 305 | 422 | 361 | 369 | 172 | 392 | 460 | 309 | 205 | 267 | 297 | 157 | 111 | 244 | 301 | 377 | |
Labelled Cell Count | 298 | 153 | 102 | 219 | 360 | 315 | 481 | 360 | 493 | 453 | 443 | 173 | 396 | 467 | 336 | 227 | 293 | 297 | 157 | 111 | 244 | 301 | 377 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.3 | 6.1 | 5.4 | 6.2 | 4.7 | 5.1 | – | 4.5 | 4.3 | 4.0 | 4.1 | 5.5 | 4.8 | 5.7 | 5.9 | 6.5 | 6.4 | 5.6 | 5.3 | 5.4 |
Std Deviation (1e-4) | 0.7 | 0.8 | 2.7 | 1.2 | 1.7 | 2.8 | 1.5 | – | 1.7 | 1.0 | 0.9 | 0.9 | 1.5 | 1.3 | 1.6 | 1.0 | 1.4 | 1.4 | 3.7 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.18 | 0.02 | -0.39 | -0.27 | – | -0.44 | -0.5 | -0.6 | -0.57 | -0.15 | -0.35 | -0.1 | -0.05 | 0.08 | 0.07 | -0.12 | -0.2 | -0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.3 | -0.4 | -1.8 | -1.5 | – | -3.5 | -4.5 | -5.6 | -5.2 | 0.6 | 2.0 | 2.2 | -0.8 | -0.2 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |