Standard name
Description Protein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 1.0 0.97 0.93 0.94 – 0.85 0.79 0.71 0.74 0.99 1.0 1.0 0.96 0.98 0.97 0.95 0.92 0.95 0.94 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0.05 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.14 0.06 – 0.25 0.29 0.44 0.39 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 1 0 0 0 0 0 0 0 1 0 0 1 0 1
Bud 0 0 0 0 0 0 1 – 2 3 7 6 0 1 1 1 0 0 0 0 0 2 1 8
Bud Neck 0 0 1 0 1 0 1 – 0 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 1 1 1 3 – 4 4 3 1 0 0 2 4 10 8 0 0 0 0 0 0
Cytoplasm 287 148 97 216 342 266 426 – 258 334 258 272 170 391 459 298 200 259 280 145 105 229 277 333
Endoplasmic Reticulum 1 0 0 0 0 1 0 – 0 0 1 1 1 0 1 17 11 15 0 0 0 1 4 1
Endosome 0 0 0 0 0 0 0 – 2 2 1 2 0 0 0 2 0 1 4 1 1 1 1 6
Golgi 0 1 0 0 1 0 0 – 0 0 0 0 0 0 2 1 0 1 3 3 0 0 2 2
Mitochondria 1 4 0 2 4 39 25 – 75 122 158 144 1 4 1 4 2 2 2 1 1 1 5 6
Nucleus 0 0 3 0 0 3 2 – 3 4 4 6 0 0 0 1 0 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 1 2 0 1 0 0 0 2 1 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 1 1 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 3 – 3 3 1 2 0 0 0 0 0 0 1 0 0 1 1 4
Vac/Vac Membrane 8 0 0 0 10 4 19 – 12 17 15 8 1 0 1 5 3 5 1 1 0 1 1 4
Unique Cell Count 297 151 99 217 352 286 452 305 422 361 369 172 392 460 309 205 267 297 157 111 244 301 377
Labelled Cell Count 298 153 102 219 360 315 481 360 493 453 443 173 396 467 336 227 293 297 157 111 244 301 377


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.3 6.1 5.4 6.2 4.7 5.1 – 4.5 4.3 4.0 4.1 5.5 4.8 5.7 5.9 6.5 6.4 5.6 5.3 5.4
Std Deviation (1e-4) 0.7 0.8 2.7 1.2 1.7 2.8 1.5 – 1.7 1.0 0.9 0.9 1.5 1.3 1.6 1.0 1.4 1.4 3.7 1.7 1.8
Intensity Change (Log2) – – – -0.18 0.02 -0.39 -0.27 – -0.44 -0.5 -0.6 -0.57 -0.15 -0.35 -0.1 -0.05 0.08 0.07 -0.12 -0.2 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 -0.4 -1.8 -1.5 – -3.5 -4.5 -5.6 -5.2 0.6 2.0 2.2 -0.8 -0.2 -0.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0