Standard name
Description Delta subunit of the oligosaccharyl transferase glycoprotein complex; complex is required for N-linked glycosylation of proteins in the endoplasmic reticulum
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0.07 0.11 0.2 0.17 0.14 0.15 0.12 0.18 0.2 0.19 0.19 0 0.06 0.1 – 0.29 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 0.12 0.05 0.06 0 0.05 0 0.08 0.07 0.11 0.11 0.12 0.13 0.06 0.14 0.26 – 0.35 – 0 0 0 0 0 0
Cytoplasm 0.12 0.1 0 0.07 0.05 0.1 0.06 0.07 0.06 0 0.09 0.09 0.06 0 0.06 – 0.24 – 0 0 0 0 0 0
Endoplasmic Reticulum 0.8 0.85 0.89 0.74 0.79 0.83 0.81 0.71 0.72 0.73 0.66 0.65 0.92 0.93 0.83 – 0 – 0.9 0.78 0.91 0.86 0.86 0.84
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Golgi 0.05 0 0.09 0.3 0.2 0 0 0 0 0.05 0 0 0 0.05 0 – 0.12 – 0 0 0 0 0 0
Mitochondria 0.05 0 0.12 0.19 0.18 0.16 0.21 0.2 0.24 0.23 0.35 0.35 0 0 0 – 0.12 – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – 0.06 – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 11 26 41 43 42 80 56 82 108 70 105 3 14 20 – 5 – 2 4 8 4 5 6
Bud 0 4 4 0 4 5 7 5 9 6 16 15 0 1 0 – 0 – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – – –
Cell Periphery 12 8 13 6 12 12 40 34 50 62 44 71 9 34 53 – 6 – 1 2 2 0 0 5
Cytoplasm 12 15 9 14 14 30 31 34 28 24 32 49 10 11 12 – 4 – 1 7 0 0 1 3
Endoplasmic Reticulum 78 131 205 151 203 250 431 332 322 402 246 353 148 229 170 – 0 – 96 152 217 103 112 139
Endosome 0 0 0 0 1 2 0 2 0 0 0 0 0 0 0 – 0 – 0 3 1 0 0 0
Golgi 5 4 21 60 51 7 17 12 8 26 8 13 6 12 1 – 2 – 1 4 0 4 4 3
Mitochondria 5 4 28 38 46 47 110 94 110 128 128 188 0 0 0 – 2 – 1 7 3 2 2 1
Nucleus 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Nuclear Periphery 2 1 1 3 1 2 13 12 14 17 13 19 0 5 2 – 0 – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – 0 0 0 0 0 0
Vac/Vac Membrane 0 2 1 6 10 4 10 24 12 16 7 7 2 2 2 – 1 – 1 8 1 0 1 1
Unique Cell Count 97 154 231 203 256 301 531 467 449 553 371 544 161 247 205 17 108 195 239 120 130 166
Labelled Cell Count 119 180 308 319 385 401 740 605 635 789 564 820 178 308 260 20 108 195 239 120 130 166


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 18.8 26.0 17.5 16.4 15.9 14.2 13.8 13.8 14.0 13.7 13.4 13.6 22.8 22.1 21.6 – 24.2 – 23.3 22.7 24.3
Std Deviation (1e-4) 4.6 5.2 3.3 3.0 3.3 2.5 2.4 2.5 2.6 2.9 2.8 3.0 3.9 4.1 4.1 – 5.1 – 4.7 4.8 5.4
Intensity Change (Log2) – – – -0.09 -0.14 -0.3 -0.34 -0.34 -0.32 -0.35 -0.38 -0.37 0.38 0.34 0.3 – 0.47 – 0.41 0.38 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.6 1.8 0.9 1.4 0.3 2.4 2.8 2.5 2.7 -3.5 -2.2 -0.5 – 0 –
Bud 0 0 0 0 0 0 0 1.7 0.8 0 0 0 – 0 –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Cell Periphery -1.4 -0.5 -0.9 1.0 0.8 2.3 2.4 2.5 3.0 0 3.0 5.9 – 0 –
Cytoplasm 1.4 0.8 2.7 1.1 1.7 1.3 0.3 2.2 2.5 1.1 0.3 1.0 – 0 –
Endoplasmic Reticulum -3.9 -2.8 -1.8 -2.6 -5.2 -5.0 -4.9 -6.2 -6.8 1.0 1.5 -1.7 – -9.3 –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Golgi 5.5 3.4 -3.5 -3.4 -3.8 -4.5 -2.4 -3.9 -4.2 -2.1 -1.8 -4.1 – 0 –
Mitochondria 1.9 1.8 1.1 2.8 2.6 3.8 3.5 6.1 6.4 -4.6 -5.6 -5.2 – 0 –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Nuclear Periphery 0 0 0 0 0 2.3 2.3 2.4 2.5 0 0 0 – 0 –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 –
Vacuole 0 2.6 0 0 3.1 0 2.2 0 0 0 0 0 – 0 –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Swp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available