Standard name | |
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Description | Delta subunit of the oligosaccharyl transferase glycoprotein complex; complex is required for N-linked glycosylation of proteins in the endoplasmic reticulum |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 5 | 11 | 26 | 41 | 43 | 42 | 80 | 56 | 82 | 108 | 70 | 105 | 3 | 14 | 20 | – | 5 | – | 2 | 4 | 8 | 4 | 5 | 6 |
Bud | 0 | 4 | 4 | 0 | 4 | 5 | 7 | 5 | 9 | 6 | 16 | 15 | 0 | 1 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – | – |
Cell Periphery | 12 | 8 | 13 | 6 | 12 | 12 | 40 | 34 | 50 | 62 | 44 | 71 | 9 | 34 | 53 | – | 6 | – | 1 | 2 | 2 | 0 | 0 | 5 |
Cytoplasm | 12 | 15 | 9 | 14 | 14 | 30 | 31 | 34 | 28 | 24 | 32 | 49 | 10 | 11 | 12 | – | 4 | – | 1 | 7 | 0 | 0 | 1 | 3 |
Endoplasmic Reticulum | 78 | 131 | 205 | 151 | 203 | 250 | 431 | 332 | 322 | 402 | 246 | 353 | 148 | 229 | 170 | – | 0 | – | 96 | 152 | 217 | 103 | 112 | 139 |
Endosome | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 3 | 1 | 0 | 0 | 0 |
Golgi | 5 | 4 | 21 | 60 | 51 | 7 | 17 | 12 | 8 | 26 | 8 | 13 | 6 | 12 | 1 | – | 2 | – | 1 | 4 | 0 | 4 | 4 | 3 |
Mitochondria | 5 | 4 | 28 | 38 | 46 | 47 | 110 | 94 | 110 | 128 | 128 | 188 | 0 | 0 | 0 | – | 2 | – | 1 | 7 | 3 | 2 | 2 | 1 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 2 | 1 | 1 | 3 | 1 | 2 | 13 | 12 | 14 | 17 | 13 | 19 | 0 | 5 | 2 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 2 | 1 | 6 | 10 | 4 | 10 | 24 | 12 | 16 | 7 | 7 | 2 | 2 | 2 | – | 1 | – | 1 | 8 | 1 | 0 | 1 | 1 |
Unique Cell Count | 97 | 154 | 231 | 203 | 256 | 301 | 531 | 467 | 449 | 553 | 371 | 544 | 161 | 247 | 205 | 17 | 108 | 195 | 239 | 120 | 130 | 166 | ||
Labelled Cell Count | 119 | 180 | 308 | 319 | 385 | 401 | 740 | 605 | 635 | 789 | 564 | 820 | 178 | 308 | 260 | 20 | 108 | 195 | 239 | 120 | 130 | 166 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 18.8 | 26.0 | 17.5 | 16.4 | 15.9 | 14.2 | 13.8 | 13.8 | 14.0 | 13.7 | 13.4 | 13.6 | 22.8 | 22.1 | 21.6 | – | 24.2 | – | 23.3 | 22.7 | 24.3 |
Std Deviation (1e-4) | 4.6 | 5.2 | 3.3 | 3.0 | 3.3 | 2.5 | 2.4 | 2.5 | 2.6 | 2.9 | 2.8 | 3.0 | 3.9 | 4.1 | 4.1 | – | 5.1 | – | 4.7 | 4.8 | 5.4 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.14 | -0.3 | -0.34 | -0.34 | -0.32 | -0.35 | -0.38 | -0.37 | 0.38 | 0.34 | 0.3 | – | 0.47 | – | 0.41 | 0.38 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 2.6 | 1.8 | 0.9 | 1.4 | 0.3 | 2.4 | 2.8 | 2.5 | 2.7 | -3.5 | -2.2 | -0.5 | – | 0 | – |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0.8 | 0 | 0 | 0 | – | 0 | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Cell Periphery | -1.4 | -0.5 | -0.9 | 1.0 | 0.8 | 2.3 | 2.4 | 2.5 | 3.0 | 0 | 3.0 | 5.9 | – | 0 | – |
Cytoplasm | 1.4 | 0.8 | 2.7 | 1.1 | 1.7 | 1.3 | 0.3 | 2.2 | 2.5 | 1.1 | 0.3 | 1.0 | – | 0 | – |
Endoplasmic Reticulum | -3.9 | -2.8 | -1.8 | -2.6 | -5.2 | -5.0 | -4.9 | -6.2 | -6.8 | 1.0 | 1.5 | -1.7 | – | -9.3 | – |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Golgi | 5.5 | 3.4 | -3.5 | -3.4 | -3.8 | -4.5 | -2.4 | -3.9 | -4.2 | -2.1 | -1.8 | -4.1 | – | 0 | – |
Mitochondria | 1.9 | 1.8 | 1.1 | 2.8 | 2.6 | 3.8 | 3.5 | 6.1 | 6.4 | -4.6 | -5.6 | -5.2 | – | 0 | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.3 | 2.4 | 2.5 | 0 | 0 | 0 | – | 0 | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Vacuole | 0 | 2.6 | 0 | 0 | 3.1 | 0 | 2.2 | 0 | 0 | 0 | 0 | 0 | – | 0 | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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