Standard name
Description Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.21 0.2 0.2 0.16 0.09 0.11 0.09 0.08 0.08 0.11 0.29 0.28 0.36 0.14 0.13 0.14 0.05 0.07 0.05 0.19 0.21 0.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.53 0.29 0.14 0.46 0.35 0.63 0.71 0.65 0.66 0.76 0.8 0.72 0 0 0 0.07 0.08 0.06 0 0 0 0 0 0
Nucleus 0.58 0.76 0.64 0.42 0.45 0.46 0.51 0.55 0.54 0.51 0.5 0.49 0.66 0.61 0.56 0.59 0.55 0.55 0.65 0.69 0.54 0.57 0.37 0.32
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Vac/Vac Membrane 0.15 0.17 0.11 0.17 0.19 0.1 0.05 0.05 0.06 0.05 0 0.05 0.08 0.15 0.14 0.23 0.34 0.33 0.22 0.18 0.28 0.14 0.3 0.31
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
Bud 0 0 1 0 3 1 13 7 10 14 9 9 0 0 0 1 0 0 0 0 1 2 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0 0 1 4
Bud Site 0 0 0 0 0 2 9 9 4 24 9 8 0 0 0 0 1 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 4 37 39 41 49 45 59 36 43 24 35 91 90 177 34 19 12 5 4 10 16 77 65
Endoplasmic Reticulum 1 0 3 1 0 0 0 0 0 0 0 0 3 1 3 12 5 4 0 0 0 0 0 1
Endosome 0 0 1 3 7 3 0 1 2 1 0 0 3 4 2 12 4 7 0 1 3 0 2 5
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 1 0 1 0
Mitochondria 39 12 24 91 71 196 367 342 255 408 233 230 2 3 2 17 11 5 1 1 8 0 11 13
Nucleus 43 32 113 82 92 141 262 288 210 273 147 158 209 197 277 144 79 48 60 51 115 48 135 107
Nuclear Periphery 0 0 0 1 0 3 0 6 0 2 3 0 0 0 0 3 0 0 0 0 0 0 0 0
Nucleolus 0 0 1 1 0 1 3 4 3 3 3 4 0 0 0 0 1 0 0 0 0 0 0 3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 3 2 2 0 1 1 1 0 0 0 0 1 0 5 2 15 17
Vac/Vac Membrane 11 7 19 33 38 31 27 25 24 28 10 17 25 49 67 57 49 29 20 13 59 11 111 103
Unique Cell Count 74 42 177 197 204 309 514 525 386 540 293 320 315 324 491 243 143 87 94 74 213 85 370 335
Labelled Cell Count 97 55 200 252 253 427 726 744 546 799 438 463 336 345 528 283 170 105 94 74 213 85 370 335


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 4.3 4.3 4.0 4.5 3.7 3.4 3.4 3.5 3.2 3.2 3.2 5.5 5.4 5.3 6.8 6.6 6.4 5.4 5.6 6.0
Std Deviation (1e-4) 0.5 0.7 1.0 1.5 2.5 1.8 1.2 1.1 1.9 1.3 1.7 1.8 0.9 1.0 0.9 1.8 1.4 1.1 1.3 1.3 1.7
Intensity Change (Log2) -0.1 0.06 -0.24 -0.36 -0.35 -0.29 -0.42 -0.46 -0.44 0.33 0.33 0.3 0.64 0.6 0.57 0.33 0.37 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.3 5.1 4.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available