Standard name
Description cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
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ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.77 0.57 0.23 0.22 0.35 0.57 0.49 0.49 0.48 0.56 0.49 0.59 0.11 0.22 0.2 0.18 0.09 0.12 0.68 0.46 0.16 0.16 0.15 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.07 0 0 0 0 0 0 0 0.1 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0 0.13 0.05 0.07 0.12 0.09 0.18 0.21 0.22 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0.07 0.39 0.31 0.14 0.09 0.13 0.14 0.13 0.11 0.11 0.06 0.5 0.55 0.57 0.36 0.32 0.31 0.14 0.38 0.6 0.54 0.59 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0
Vac/Vac Membrane 0.18 0.4 0.49 0.47 0.51 0.36 0.35 0.37 0.33 0.26 0.32 0.27 0.42 0.3 0.29 0.5 0.61 0.57 0.08 0.07 0.14 0.11 0.1 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 1 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud 0 0 0 2 0 1 3 4 3 7 8 5 0 0 0 0 0 0 5 3 5 3 15 18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 3
Bud Site 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 1 2 1 2 6 3 6 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 116 52 42 52 130 196 332 259 252 406 237 366 24 79 90 22 13 14 121 52 35 9 48 33
Endoplasmic Reticulum 0 4 1 0 1 0 2 2 3 5 0 7 0 0 2 4 10 4 0 0 0 0 1 0
Endosome 0 1 3 7 28 7 9 7 5 1 2 1 21 9 15 3 2 3 3 1 3 1 2 3
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 3 5 2 1 0 3 0 1 1
Mitochondria 19 3 3 32 17 25 83 49 94 154 108 96 1 0 0 0 2 2 2 0 1 0 2 1
Nucleus 3 6 70 75 53 32 91 72 68 78 55 37 104 200 252 44 46 35 24 44 135 31 183 165
Nuclear Periphery 0 0 0 1 1 1 1 1 3 1 2 2 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 1 5 3 0 1 0 0 0 0 0 1 1 0 0 0 1 0 3 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 1 5 3 14 10
Vac/Vac Membrane 27 37 88 113 190 123 236 192 174 191 156 168 88 109 129 62 86 65 15 8 31 6 31 24
Unique Cell Count 150 92 181 240 375 342 678 526 524 729 488 617 210 361 442 123 142 114 179 116 227 59 314 275
Labelled Cell Count 166 103 208 282 420 387 765 590 604 853 571 691 240 397 489 140 165 125 179 116 227 59 314 275


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 6.8 6.4 5.9 6.8 7.4 7.7 8.0 7.9 8.0 8.1 8.6 7.8 8.2 8.6 14.2 15.1 14.8 6.5 7.9 8.5
Std Deviation (1e-4) 0.8 1.5 1.4 1.5 1.7 1.5 1.8 2.1 2.1 2.3 2.6 2.8 1.1 1.5 1.6 3.0 3.0 2.9 0.9 1.4 1.5
Intensity Change (Log2) – – – -0.1 0.1 0.22 0.27 0.34 0.3 0.32 0.35 0.44 0.29 0.36 0.44 1.16 1.25 1.21 0.04 0.32 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.4 2.7 7.5 6.2 6.1 5.9 7.8 5.9 8.5 -3.1 -0.3 -0.8 -1.1 -3.3 -2.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.8 0 0 0 0 0 0 0 3.4 0 1.2 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 1.7 2.7 4.2 3.4 5.5 6.2 6.3 5.0 0 0 0 0 0 0
Nucleus -1.6 -6.5 -8.0 -7.7 -7.2 -7.5 -9.1 -8.1 -11.3 2.2 3.7 4.2 -0.5 -1.2 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 0.5 -2.8 -3.4 -2.9 -3.7 -5.9 -4.0 -5.4 -1.3 -4.2 -4.6 0.3 2.1 1.4