Standard name | |
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Human Ortholog | |
Description | Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 |
Bud | 0 | 2 | 0 | 0 | 1 | 7 | 11 | 14 | 6 | 18 | 7 | 9 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 7 | 3 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 6 |
Bud Site | 1 | 0 | 0 | 0 | 1 | 13 | 18 | 18 | 11 | 23 | 22 | 15 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 16 | 21 | 23 | 27 | 42 | 54 | 68 | 56 | 36 | 51 | 50 | 23 | 47 | 43 | 17 | 17 | 12 | 1 | 3 | 0 | 1 | 1 | 3 |
Endoplasmic Reticulum | 0 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 4 | 4 | 2 | 0 | 0 | 1 | 2 | 3 | 1 |
Endosome | 0 | 2 | 3 | 1 | 11 | 2 | 3 | 6 | 11 | 11 | 21 | 19 | 2 | 1 | 1 | 2 | 2 | 5 | 2 | 6 | 14 | 4 | 5 | 2 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 1 | 1 | 5 | 0 | 1 | 0 |
Mitochondria | 36 | 73 | 30 | 23 | 45 | 335 | 311 | 300 | 291 | 292 | 372 | 311 | 4 | 2 | 1 | 13 | 11 | 10 | 0 | 10 | 3 | 7 | 9 | 5 |
Nucleus | 261 | 436 | 414 | 340 | 355 | 446 | 521 | 482 | 392 | 384 | 346 | 348 | 141 | 205 | 160 | 122 | 131 | 131 | 245 | 427 | 350 | 129 | 191 | 218 |
Nuclear Periphery | 0 | 0 | 3 | 3 | 1 | 1 | 13 | 3 | 5 | 0 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 6 | 0 | 2 | 1 |
Nucleolus | 8 | 7 | 18 | 7 | 13 | 34 | 36 | 32 | 21 | 30 | 20 | 17 | 4 | 3 | 0 | 2 | 2 | 8 | 14 | 24 | 62 | 21 | 41 | 36 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 6 | 3 | 1 | 4 | 3 | 11 | 19 | 4 | 17 | 10 | 11 | 1 | 4 | 0 | 3 | 0 | 4 | 2 | 11 | 8 | 5 | 15 | 13 |
Vac/Vac Membrane | 1 | 11 | 4 | 1 | 17 | 13 | 13 | 11 | 29 | 24 | 31 | 38 | 5 | 1 | 2 | 9 | 12 | 8 | 13 | 29 | 31 | 18 | 30 | 49 |
Unique Cell Count | 276 | 492 | 459 | 376 | 433 | 657 | 754 | 726 | 618 | 602 | 635 | 617 | 175 | 256 | 208 | 149 | 168 | 162 | 285 | 530 | 498 | 200 | 311 | 347 |
Labelled Cell Count | 312 | 557 | 499 | 399 | 476 | 896 | 993 | 954 | 826 | 836 | 883 | 822 | 182 | 264 | 211 | 173 | 183 | 184 | 285 | 530 | 498 | 200 | 311 | 347 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.2 | 5.0 | 5.5 | 5.2 | 5.1 | 3.9 | 4.1 | 4.3 | 4.3 | 4.0 | 4.0 | 3.9 | 5.9 | 5.9 | 6.2 | 6.4 | 6.4 | 6.6 | 5.3 | 5.5 | 5.9 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.7 | 1.6 | 2.0 | 1.7 | 1.6 | 1.5 | 3.3 | 1.0 | 3.0 | 1.1 | 1.0 | 1.2 | 1.5 | 1.5 | 1.3 | 1.3 | 1.1 | 0.9 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.11 | -0.5 | -0.42 | -0.36 | -0.38 | -0.47 | -0.46 | -0.5 | 0.08 | 0.08 | 0.17 | 0.22 | 0.21 | 0.25 | -0.06 | -0.0 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.0 | 1.1 | 1.3 | 1.8 | 3.0 | 2.8 | 1.0 | 2.3 | 2.3 | 3.8 | 6.0 | 6.5 | 3.0 | 2.6 | 1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -0.2 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -3.6 | -3.4 | 0.9 | 0 | -0.2 |
Nucleus | 0.1 | -3.6 | -8.7 | -8.5 | -9.3 | -10.0 | -9.9 | -12.7 | -12.1 | -3.3 | -3.8 | -4.6 | -2.7 | -4.0 | -3.1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -1.7 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -2.1 | -2.9 | 0 | -1.7 | 0.6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.0 | 1.5 | 1.2 | 1.0 | 3.6 | 3.1 | 3.7 | 4.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasmic Foci | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Eisosomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Lipid Particles | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
None | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Punctate Nuclear | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.8575 | 9.0557 | 7.7535 | 13.4765 | 9.598 | 9.3457 | 9.8605 | 12.3597 | 11.4144 | 13.5471 |
Translational Efficiency | 1.1915 | 1.1186 | 1.6266 | 0.7326 | 1.2842 | 1.3651 | 1.0776 | 0.9854 | 0.9826 | 0.7231 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | – |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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