Standard name
Description Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.06 0.06 0.07 0.09 0.09 0.06 0.08 0.08 0.13 0.18 0.21 0.11 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0.15 0.07 0.06 0.1 0.51 0.41 0.41 0.47 0.49 0.59 0.5 0 0 0 0.09 0.07 0.06 0 0 0 0 0 0
Nucleus 0.95 0.89 0.9 0.9 0.82 0.68 0.69 0.66 0.63 0.64 0.54 0.56 0.81 0.8 0.77 0.82 0.78 0.81 0.86 0.81 0.7 0.65 0.62 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0.05 0 0 0.05 0 0 0 0 0 0 0 0.05 0.05 0.05 0.12 0.11 0.13 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0 0.05 0.06 0 0 0 0.06 0.07 0.05 0.05 0.06 0.06 0.09 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 0
Bud 0 2 0 0 1 7 11 14 6 18 7 9 0 0 0 0 1 2 4 7 3 2 2 3
Bud Neck 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 1 2 1 2 6
Bud Site 1 0 0 0 1 13 18 18 11 23 22 15 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 16 21 23 27 42 54 68 56 36 51 50 23 47 43 17 17 12 1 3 0 1 1 3
Endoplasmic Reticulum 0 4 0 0 0 0 1 0 0 0 0 0 2 1 1 4 4 2 0 0 1 2 3 1
Endosome 0 2 3 1 11 2 3 6 11 11 21 19 2 1 1 2 2 5 2 6 14 4 5 2
Golgi 0 0 3 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2 1 1 5 0 1 0
Mitochondria 36 73 30 23 45 335 311 300 291 292 372 311 4 2 1 13 11 10 0 10 3 7 9 5
Nucleus 261 436 414 340 355 446 521 482 392 384 346 348 141 205 160 122 131 131 245 427 350 129 191 218
Nuclear Periphery 0 0 3 3 1 1 13 3 5 0 3 4 0 0 0 0 1 0 0 1 6 0 2 1
Nucleolus 8 7 18 7 13 34 36 32 21 30 20 17 4 3 0 2 2 8 14 24 62 21 41 36
Peroxisomes 0 0 0 0 1 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 6 3 1 4 3 11 19 4 17 10 11 1 4 0 3 0 4 2 11 8 5 15 13
Vac/Vac Membrane 1 11 4 1 17 13 13 11 29 24 31 38 5 1 2 9 12 8 13 29 31 18 30 49
Unique Cell Count 276 492 459 376 433 657 754 726 618 602 635 617 175 256 208 149 168 162 285 530 498 200 311 347
Labelled Cell Count 312 557 499 399 476 896 993 954 826 836 883 822 182 264 211 173 183 184 285 530 498 200 311 347


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.0 5.5 5.2 5.1 3.9 4.1 4.3 4.3 4.0 4.0 3.9 5.9 5.9 6.2 6.4 6.4 6.6 5.3 5.5 5.9
Std Deviation (1e-4) 0.7 0.8 1.7 1.6 2.0 1.7 1.6 1.5 3.3 1.0 3.0 1.1 1.0 1.2 1.5 1.5 1.3 1.3 1.1 0.9 1.1
Intensity Change (Log2) -0.08 -0.11 -0.5 -0.42 -0.36 -0.38 -0.47 -0.46 -0.5 0.08 0.08 0.17 0.22 0.21 0.25 -0.06 -0.0 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.1 1.3 1.8 3.0 2.8 1.0 2.3 2.3 3.8 6.0 6.5 3.0 2.6 1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 2.3 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 2.1 0 0 0 0 0 0 0 -2.1 -3.6 -3.4 0.9 0 -0.2
Nucleus 0.1 -3.6 -8.7 -8.5 -9.3 -10.0 -9.9 -12.7 -12.1 -3.3 -3.8 -4.6 -2.7 -4.0 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.7 -0.7 0 0 0 0 0 0 0 -1.0 -2.1 -2.9 0 -1.7 0.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.0 1.5 1.2 1.0 3.6 3.1 3.7 4.4 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ubp8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available