Standard name
Description Translation initiation factor eIF1A; essential protein that forms a complex with Sui1p (eIF1) and the 40S ribosomal subunit and scans for the start codon; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 – 0 0 0 0.05 0.05 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 – 0 0 0 0.13 0.17 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.94 – 0.88 0.92 0.88 – 0.92 0.9 0.7 0.78 0.64 – – – 0.89 0.92 0.9 0.96 0.93 0.88 0.82 0.85 0.89
Endoplasmic Reticulum 0 0 – 0 0.07 0.07 – 0 0.07 0.08 0.05 0.06 – – – 0 0 0 0 0 0.05 0.09 0.05 0.06
Endosome 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0.06 0 0 0 0 0 0
Mitochondria 0 0 – 0.06 0 0.08 – 0.08 0.05 0.22 0.17 0.28 – – – 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0.06 0 0 – 0 0 0.05 0 0 – – – 0 0 0 0 0 0 0.08 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 – 1 0 1 3 5 – – – 4 1 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 – 0 0 0 1 2 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 1 4 – 0 2 1 – 3 1 1 8 16 – – – 8 1 4 0 0 0 0 0 0
Cytoplasm 59 115 – 14 82 52 – 89 36 58 49 60 – – – 165 159 139 68 120 35 10 23 40
Endoplasmic Reticulum 0 5 – 0 6 4 – 4 3 7 3 6 – – – 4 4 6 0 2 1 1 1 2
Endosome 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 2 1 – 3 0 1 0 1 – – – 6 2 9 0 0 0 0 0 0
Mitochondria 0 0 – 1 1 5 – 8 2 18 11 26 – – – 6 6 3 0 0 1 0 0 0
Nucleus 0 0 – 0 1 0 – 0 0 0 0 0 – – – 1 1 1 1 1 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 – 0 1 0 2 1 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 1 – 0 1 1 1 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 1 1 0 – 0 0 4 0 1 – – – 4 7 1 0 3 0 1 0 1
Unique Cell Count 59 122 16 89 59 97 40 83 63 94 186 172 154 71 130 40 13 27 45
Labelled Cell Count 60 124 16 95 64 108 44 91 78 118 198 181 163 71 130 40 13 27 45


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 39.9 49.6 – 23.6 26.1 20.5 – 19.0 16.5 17.3 15.6 15.8 – – – 42.0 34.6 37.3 – – –
Std Deviation (1e-4) 7.3 8.8 – 9.5 7.3 6.6 – 4.6 4.9 4.6 5.4 4.9 – – – 17.1 13.5 12.5 – – –
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 23.8303 41.2423 35.2771 37.6945 27.9839 38.7234 28.4882 42.8307 34.2199 29.0612 25.3299 37.0393 22.5164 46.5863 37.2602 22.1639 25.043 39.4442
Actin 0.0075 0.0166 0.0098 0.0153 0.0025 0.0051 0.0471 0.0005 0.0359 0.0322 0.0058 0.0027 0.0644 0.0011 0.0065 0.0173 0.0018 0.0085
Bud 0.0037 0.0049 0.0059 0.0103 0.0067 0.0018 0.0045 0.0002 0.0063 0.0122 0.0047 0.0012 0.0042 0.0084 0.0008 0.0021 0.0026 0.0046
Bud Neck 0.0013 0.0091 0.0032 0.001 0.0007 0.0017 0.0013 0.0002 0.0007 0.0021 0.0007 0.0009 0.001 0.0005 0.0005 0.0001 0.001 0.0059
Bud Periphery 0.0106 0.0025 0.0042 0.0039 0.002 0.0016 0.0085 0 0.0169 0.0143 0.0034 0.0008 0.0082 0.0027 0.0002 0.0018 0.0042 0.0066
Bud Site 0.0123 0.0155 0.016 0.0006 0.0005 0.0002 0.0066 0.0002 0.005 0.0286 0.001 0.0001 0.0043 0.0038 0.0005 0.0008 0.0008 0.0007
Cell Periphery 0.0104 0.0017 0.0017 0.0014 0.0001 0.0005 0.0023 0.0001 0.0027 0.0038 0.0006 0.0002 0.0017 0.0015 0.0002 0.0005 0.0023 0.0006
Cytoplasm 0.5149 0.8117 0.7362 0.5432 0.4248 0.6316 0.6566 0.9889 0.8275 0.8074 0.6082 0.9041 0.3597 0.8991 0.7614 0.2165 0.4507 0.6497
Cytoplasmic Foci 0.0233 0.0089 0.0225 0.0082 0.0279 0.0129 0.0066 0.001 0.0051 0.0045 0.0069 0.0065 0.0301 0.0115 0.0512 0.0079 0.0081 0.0193
Eisosomes 0.0002 0.0001 0.0001 0.0004 0 0.0001 0.0004 0 0.0004 0.0003 0 0.0001 0.0008 0 0 0.0015 0.0001 0.0001
Endoplasmic Reticulum 0.0571 0.0286 0.0049 0.0082 0.0069 0.0513 0.0571 0.0027 0.007 0.0076 0.0259 0.006 0.1127 0.0437 0.0301 0.0062 0.0386 0.0749
Endosome 0.0716 0.0164 0.0403 0.0425 0.2463 0.089 0.0448 0.0011 0.0135 0.0226 0.1926 0.0243 0.0804 0.0051 0.0577 0.0776 0.1076 0.038
Golgi 0.0152 0.0113 0.0145 0.0165 0.0321 0.0294 0.0067 0.0003 0.0062 0.0065 0.0493 0.0044 0.0159 0.0014 0.02 0.0108 0.0079 0.0139
Lipid Particles 0.0109 0.0003 0.0103 0.0002 0.0004 0.0002 0.0019 0 0.001 0.0015 0.0006 0.0001 0.0444 0.0004 0.0417 0.0082 0.0075 0.0002
Mitochondria 0.0203 0.0293 0.0288 0.0203 0.0628 0.1141 0.0215 0.0001 0.0362 0.0121 0.0214 0.0333 0.03 0.0029 0.004 0.4367 0.0356 0.1553
None 0.0146 0.0005 0.0011 0.0009 0.0033 0.0008 0.0034 0.0001 0.0023 0.0025 0.001 0.0004 0.01 0.0007 0.0006 0.0021 0.0045 0.0009
Nuclear Periphery 0.0594 0.0021 0.003 0.0044 0.0701 0.0166 0.0707 0.0003 0.0046 0.0064 0.0113 0.0017 0.0644 0.0023 0.0022 0.01 0.022 0.0047
Nucleolus 0.0035 0.0021 0.0031 0.0002 0.0008 0.0001 0.0007 0 0.0003 0.001 0.0001 0 0.0015 0.0003 0.0001 0.0002 0.0012 0.0001
Nucleus 0.071 0.0021 0.0058 0.0042 0.0342 0.005 0.0193 0.0005 0.005 0.0073 0.0027 0.0015 0.0136 0.0016 0.0008 0.0017 0.0073 0.0022
Peroxisomes 0.0023 0.0004 0.0278 0.0003 0.0016 0.0003 0.0005 0 0.0005 0.0006 0.0002 0.0001 0.0203 0.0006 0.0139 0.0024 0.0005 0.0014
Punctate Nuclear 0.0062 0.0001 0.0027 0.0001 0.0037 0.0001 0.0018 0 0.001 0.0006 0.0002 0.0001 0.0041 0.0002 0.0017 0.0002 0.0003 0.0006
Vacuole 0.0566 0.0277 0.0427 0.2212 0.0316 0.0194 0.0226 0.0031 0.0161 0.0202 0.0399 0.007 0.0826 0.01 0.0039 0.1196 0.1579 0.0051
Vacuole Periphery 0.0273 0.0083 0.0151 0.0966 0.041 0.0184 0.0153 0.0005 0.0055 0.0056 0.0234 0.0044 0.0456 0.0021 0.002 0.0759 0.1375 0.0066

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 441.8394 412.0576 549.7424 441.4769 445.9051 486.9478 380.4726 364.2459 457.461 362.3869
Translational Efficiency 2.1977 2.3982 1.7011 2.7396 2.2076 1.9606 2.0078 2.0889 1.6965 1.9417

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘