Standard name
Description Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.08 0.08 0.12 0.07 0.12 0.07 0 0 0 0.05 0 – 0 0 0 0 0 0
Bud Neck 0 0 0.16 0.11 0.17 0.09 0.1 0.12 0.12 0.08 0.09 0.11 0.13 0.13 0.08 0.1 0 – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.05 0.05 0 0.09 0.06 0.07 0 0 0 0 0 0 – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 0.05 0.1 0.12 0.1 0.14 0.15 0.19 0.21 0.18 0.15 0.15 0.16 0.13 0.19 0.23 0.1 0.22 – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0
Endosome 0 0.28 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.1 0.3 – 0.15 0.14 0.15 0.14 0.17 0.17
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Mitochondria 0.57 0.11 0 0 0 0.14 0.31 0.2 0.38 0.35 0.58 0.35 0 0 0 0.19 0.22 – 0 0 0.06 0 0 0
Nucleus 0 0.17 0.15 0.07 0 0 0 0 0 0 0.05 0 0.12 0.1 0.05 0.05 0.08 – 0 0.07 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Nucleolus 0.11 0.1 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0.05 0.14 0.16 – 0.2 0.3 0.2 0.17 0.11 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
SpindlePole 0.45 0.44 0.71 0.77 0.75 0.7 0.53 0.62 0.52 0.57 0.4 0.53 0.72 0.69 0.67 0.52 0.53 – 0.45 0.31 0.42 0.51 0.56 0.54
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 – 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 1 0 0 0 0 0 0 1 0 0 0 0 0 – 0 0 1 0 1 0
Bud 1 1 1 3 17 19 45 51 58 35 61 34 1 2 0 1 0 – 0 3 0 1 1 4
Bud Neck 1 2 31 38 84 44 57 75 61 43 42 56 37 53 32 2 1 – 2 3 2 3 9 16
Bud Site 1 0 0 3 18 23 30 19 47 30 34 20 0 0 0 0 1 – – – – – – –
Cell Periphery 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0
Cytoplasm 9 19 23 35 69 69 107 128 90 75 72 78 36 78 94 2 14 – 2 7 5 9 10 13
Endoplasmic Reticulum 2 0 1 1 1 0 0 1 0 0 0 0 1 0 0 1 1 – 0 0 1 1 0 1
Endosome 2 53 4 9 14 17 7 5 5 14 8 7 10 22 44 2 19 – 37 34 36 37 74 85
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 – 0 1 0 0 3 1
Mitochondria 111 20 4 14 17 67 172 122 189 178 287 172 1 1 1 4 14 – 6 4 14 8 10 13
Nucleus 7 32 28 23 11 14 22 20 14 15 26 17 32 40 20 1 5 – 5 15 7 3 4 2
Nuclear Periphery 2 0 0 0 0 1 0 0 1 0 0 1 0 0 0 0 0 – 1 0 1 0 0 2
Nucleolus 22 19 1 8 7 14 22 20 30 26 22 9 12 15 20 3 10 – 48 72 48 43 47 53
Peroxisomes 0 4 0 2 1 3 1 4 6 5 7 6 0 1 4 0 0 – 1 1 0 0 1 1
SpindlePole 88 84 133 264 369 326 296 379 261 290 197 263 198 289 271 11 34 – 111 75 100 133 247 273
Vac/Vac Membrane 1 4 0 1 2 1 6 1 4 3 2 6 1 1 3 0 3 – 11 10 10 7 12 15
Unique Cell Count 195 189 188 344 489 466 555 613 501 513 493 497 276 418 405 21 64 246 242 241 263 441 506
Labelled Cell Count 247 239 227 401 612 598 765 826 766 714 758 670 329 502 490 27 102 246 242 241 263 441 506


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 4.7 5.1 4.5 4.7 3.8 3.6 4.0 3.3 3.5 3.2 3.6 5.1 5.1 5.3 5.7 6.8 – 4.6 5.1 5.3
Std Deviation (1e-4) 0.5 1.5 1.5 1.1 1.5 1.3 1.4 1.4 1.0 0.9 0.9 1.1 1.1 1.1 1.7 1.4 2.9 – 1.1 1.3 1.7
Intensity Change (Log2) – – – -0.18 -0.12 -0.45 -0.51 -0.34 -0.64 -0.53 -0.69 -0.5 -0.01 -0.0 0.04 0.16 0.4 – -0.15 -0.01 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.595 0.7761 0.5197 0.2876 0.89 0.642 0.69 0.5164 0.6358 0.1962 0.0334 0.4261 -0.7844 -0.7816 -0.9233 -0.4016 -1.0832 -0.8121
Actin 0.0249 0.0001 0.0004 0.0236 0.0473 0.0042 0.0138 0.0001 0.0103 0.0001 0.0012 0.002 0.0228 0.0001 0.0011 0.0002 0.0099 0.0032
Bud 0.003 0.0001 0.0003 0.0002 0.0006 0.0001 0.0002 0.0001 0.0006 0.0001 0.0001 0.0011 0.0002 0.0005 0.0001 0.0001 0.0002 0.0001
Bud Neck 0.0069 0.0053 0.0047 0.0076 0.0153 0.0061 0.0135 0.0105 0.0058 0.0141 0.0042 0.0035 0.0046 0.0037 0.0043 0.0082 0.0031 0.0038
Bud Periphery 0.0013 0.0003 0.0004 0.0003 0.0012 0.0002 0.0005 0.0002 0.0019 0.0003 0.0005 0.0011 0.0004 0.0007 0.0003 0.0003 0.0006 0.0004
Bud Site 0.0053 0.004 0.0027 0.0018 0.003 0.0008 0.0034 0.0027 0.0045 0.0014 0.0005 0.0007 0.0026 0.0063 0.0021 0.0018 0.0009 0.0007
Cell Periphery 0.0004 0.0006 0.0008 0.0007 0.0004 0.0002 0.0004 0.0004 0.0004 0.0005 0.0002 0.0001 0.0003 0.0004 0.0004 0.0005 0.0003 0.0001
Cytoplasm 0.0055 0.005 0.0018 0.0006 0.0057 0.0023 0.0024 0.0023 0.0043 0.0003 0.0007 0.0021 0.002 0.0034 0.001 0.0005 0.0012 0.001
Cytoplasmic Foci 0.065 0.0322 0.0343 0.016 0.0648 0.0281 0.0401 0.0138 0.0213 0.0146 0.032 0.0164 0.0528 0.0198 0.0301 0.0161 0.0316 0.0196
Eisosomes 0.0005 0.0001 0.0001 0.0001 0.0008 0.0001 0.0003 0.0001 0.0003 0 0.0001 0.0001 0.0002 0 0.0001 0.0002 0.0001 0
Endoplasmic Reticulum 0.0015 0.0004 0.0002 0.0002 0.0045 0.0004 0.0013 0.0003 0.0009 0 0.0006 0.0002 0.0009 0.0003 0.0003 0.0001 0.0003 0.0006
Endosome 0.0222 0.0075 0.0064 0.0074 0.0535 0.0096 0.0275 0.0069 0.0127 0.0039 0.0088 0.0063 0.0198 0.0056 0.0092 0.0048 0.0078 0.0109
Golgi 0.0075 0.0026 0.0027 0.0026 0.0153 0.0041 0.0101 0.0014 0.0045 0.0017 0.004 0.0036 0.0067 0.002 0.0034 0.0019 0.0071 0.0032
Lipid Particles 0.1092 0.1139 0.0926 0.0928 0.1145 0.0992 0.1043 0.0391 0.0699 0.0495 0.0862 0.0603 0.1126 0.0592 0.1149 0.0574 0.1508 0.0557
Mitochondria 0.0077 0.0006 0.0039 0.0009 0.0087 0.001 0.0063 0.0005 0.009 0.0007 0.0029 0.0098 0.0012 0.0006 0.0013 0.001 0.0034 0.0006
None 0.0277 0.0288 0.0078 0.0019 0.0382 0.0025 0.0116 0.0275 0.0042 0.0008 0.0391 0.0057 0.0166 0.0292 0.0068 0.0034 0.0485 0.0103
Nuclear Periphery 0.0081 0.004 0.002 0.0026 0.0224 0.0055 0.0148 0.0023 0.0044 0.0027 0.0091 0.0062 0.0058 0.0085 0.004 0.0007 0.0029 0.0064
Nucleolus 0.2515 0.3976 0.5386 0.5018 0.2029 0.2692 0.3204 0.4715 0.508 0.6235 0.248 0.2596 0.324 0.4227 0.4882 0.5516 0.3314 0.2184
Nucleus 0.0548 0.0721 0.065 0.0693 0.0437 0.0289 0.0586 0.0995 0.0769 0.0674 0.0208 0.0363 0.0467 0.0972 0.0664 0.0568 0.0308 0.038
Peroxisomes 0.0191 0.0068 0.0051 0.0104 0.0476 0.0097 0.0252 0.0027 0.0077 0.0034 0.008 0.01 0.0113 0.0035 0.0128 0.0029 0.0426 0.0032
Punctate Nuclear 0.3731 0.3126 0.2232 0.2522 0.303 0.5265 0.3403 0.3119 0.2477 0.2112 0.5307 0.5732 0.3659 0.3325 0.2479 0.2893 0.3251 0.6221
Vacuole 0.0036 0.0047 0.0063 0.0066 0.0043 0.0013 0.0036 0.0061 0.0038 0.0034 0.0013 0.0011 0.0022 0.0035 0.0048 0.0022 0.0011 0.0012
Vacuole Periphery 0.0011 0.0004 0.0006 0.0005 0.0023 0.0003 0.0014 0.0003 0.0009 0.0004 0.001 0.0007 0.0004 0.0004 0.0006 0.0002 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 22.6107 19.7635 16.6652 15.0773 9.7719 28.1222 23.8431 17.4719 18.5603 18.6459
Translational Efficiency 0.4606 0.5958 0.4383 0.4407 0.7803 0.3883 0.3777 0.4416 0.4045 0.5431

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘