Standard name
Description U2-snRNP associated splicing factor; forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0.06 0 0 0.05 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.08 0 0.05 0.1 0.1 0.06 0.05 0.06 0.05 0.08 0 0.12 0.17 0 0.08 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.52 0.38 0.46 0.7 0.73 0.62 0.71 0.76 0.76 0 0 0 0.08 0.09 0.08 0 0 0 0 0 0
Nucleus 0.96 0.87 0.77 0.82 0.72 0.66 0.64 0.67 0.61 0.63 0.63 0.96 0.86 0.81 0.89 0.86 0.84 0.9 0.83 0.75 0.81 0.73 0.73
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.06 0.05 0.05 0.11 0.11 0.16 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Bud 0 0 1 1 2 12 7 11 9 11 10 0 0 0 0 2 2 0 0 0 1 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 1 2
Bud Site 1 0 0 3 6 30 22 18 33 16 25 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 4 17 46 51 42 38 45 31 55 9 67 113 13 27 29 1 3 2 0 4 5
Endoplasmic Reticulum 0 0 0 1 0 1 0 0 0 0 0 0 2 3 6 13 13 0 0 0 1 1 3
Endosome 1 0 0 1 0 0 1 1 1 0 0 2 0 1 2 1 2 0 0 2 2 4 4
Golgi 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 1
Mitochondria 7 0 90 124 212 353 532 484 494 434 500 3 4 2 26 30 24 1 0 4 1 5 9
Nucleus 166 55 133 266 333 334 465 524 420 359 418 414 488 541 308 298 260 161 79 143 195 252 284
Nuclear Periphery 0 0 2 5 0 1 2 3 5 5 4 0 0 0 3 0 0 0 0 0 1 0 1
Nucleolus 3 2 2 2 0 5 19 16 7 5 8 1 0 2 2 2 1 4 4 6 3 6 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0 0 0 1 0 1 0
SpindlePole 0 0 4 2 4 4 5 4 8 0 3 10 4 7 1 2 1 0 0 4 2 3 3
Vac/Vac Membrane 2 5 1 3 10 10 15 13 14 14 6 2 4 12 20 17 18 8 5 20 27 54 54
Unique Cell Count 173 63 173 325 463 503 726 780 694 572 659 431 568 670 346 348 310 179 96 192 243 348 389
Labelled Cell Count 184 67 237 425 614 801 1110 1112 1036 875 1029 442 574 682 383 392 350 179 96 192 243 348 389


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 6.3 3.9 3.8 3.8 3.4 3.1 3.4 3.1 3.1 3.2 6.0 6.0 6.0 6.3 6.2 6.1 6.4 6.8 6.9
Std Deviation (1e-4) 0.7 1.8 0.7 1.0 1.3 1.0 1.3 1.2 0.8 1.0 1.0 0.9 1.3 1.2 0.8 1.1 1.1 1.0 1.3 1.4
Intensity Change (Log2) -0.03 -0.05 -0.19 -0.3 -0.21 -0.33 -0.34 -0.28 0.62 0.64 0.64 0.7 0.67 0.65 0.72 0.8 0.82


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.5 3.2 3.2 1.9 1.5 2.1 1.7 2.8 0 3.7 4.9 0.9 2.5 2.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 -15.8 -17.8 -19.5 -11.5 -11.1 -11.0
Nucleus 1.3 -1.3 -2.6 -3.2 -2.5 -4.0 -3.4 -3.3 7.3 2.8 1.1 3.6 2.5 1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 2.8 2.5 2.8


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsh155-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available