Standard name | |
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Description | eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3); involved in the assembly of preinitiation complex and start codon selection; eIF3 is also involved in programmed stop codon readthrough |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 12 | 11 | 23 | 24 | 13 | 27 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 1 | 3 | 0 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 4 | 11 | 3 | 18 | 7 | 20 | 28 | 23 | 24 | 16 | 41 | 0 | 1 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 207 | 230 | 321 | 89 | 279 | 312 | 326 | 310 | 287 | 249 | 221 | 213 | 181 | 290 | 257 | 16 | – | 52 | 200 | 226 | 328 | 133 | 169 | 211 |
Endoplasmic Reticulum | 2 | 3 | 10 | 4 | 23 | 53 | 48 | 110 | 65 | 80 | 43 | 53 | 6 | 12 | 9 | 1 | – | 3 | 2 | 3 | 4 | 16 | 29 | 40 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 15 | 10 | 10 | 8 | 14 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 3 |
Mitochondria | 0 | 0 | 1 | 0 | 0 | 1 | 3 | 5 | 20 | 28 | 26 | 18 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 3 | 7 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 1 | 0 | 1 | 2 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 2 | 1 | 6 | 2 | 1 | 15 | 1 | 9 | 7 | 15 | 3 | 3 | 0 | 0 | – | 2 | 2 | 3 | 2 | 3 | 4 | 4 |
Unique Cell Count | 210 | 232 | 330 | 96 | 308 | 370 | 389 | 451 | 385 | 372 | 300 | 330 | 191 | 303 | 266 | 16 | 52 | 206 | 238 | 342 | 164 | 216 | 284 | |
Labelled Cell Count | 212 | 237 | 346 | 98 | 327 | 378 | 415 | 498 | 429 | 425 | 336 | 385 | 192 | 307 | 270 | 17 | 57 | 206 | 238 | 342 | 164 | 216 | 284 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 58.7 | 61.5 | 46.3 | 41.0 | 37.2 | 29.2 | 26.9 | 26.6 | 23.2 | 21.7 | 20.1 | 20.8 | 57.7 | 58.7 | 54.0 | 70.6 | – | 56.7 | 51.2 | 52.8 | 60.3 |
Std Deviation (1e-4) | 12.1 | 13.2 | 9.5 | 11.7 | 7.4 | 6.2 | 5.9 | 6.3 | 5.1 | 4.8 | 4.7 | 4.7 | 14.6 | 14.5 | 15.4 | 27.2 | – | 15.6 | 14.6 | 15.9 | 16.8 |
Intensity Change (Log2) | – | – | – | -0.17 | -0.32 | -0.66 | -0.78 | -0.8 | -1.0 | -1.09 | -1.2 | -1.15 | 0.32 | 0.34 | 0.22 | 0.61 | – | 0.29 | 0.14 | 0.19 | 0.38 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 3.2 | 0 | 4.5 | 4.7 | 3.8 | 5.3 | 0 | 0 | 0 | 0 | – | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Cell Periphery | 0 | 1.5 | -1.2 | 1.2 | 1.8 | 1.7 | 1.9 | 1.2 | 4.3 | 0 | -2.8 | -1.8 | 0 | – | 0 |
Cytoplasm | -2.1 | -3.6 | -5.8 | -6.0 | -10.0 | -8.5 | -10.3 | -8.5 | -10.7 | -1.5 | -1.1 | -0.5 | 0.7 | – | 1.2 |
Endoplasmic Reticulum | 0 | 2.5 | 5.2 | 4.6 | 8.2 | 6.0 | 7.3 | 5.1 | 5.7 | 0.1 | 0.6 | 0.2 | 0 | – | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Golgi | 0 | 0 | 0 | 0 | 2.9 | 2.5 | 2.5 | 0 | 3.4 | 0 | 0 | 0 | 0 | – | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.9 | 4.8 | 5.2 | 4.0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 |
Vacuole | 0 | 0 | 0 | 0 | 2.6 | 0 | 1.9 | 0 | 3.2 | 0 | 0 | 0 | 0 | – | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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