Standard name
Description eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3); involved in the assembly of preinitiation complex and start codon selection; eIF3 is also involved in programmed stop codon readthrough
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.06 0 0.08 0 0 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 0 0 0 0 0.06 0 0.05 0.06 0.06 0.06 0.05 0.12 0 0 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.93 0.91 0.84 0.84 0.69 0.75 0.67 0.74 0.65 0.95 0.96 0.97 1.0 – 1.0 0.97 0.95 0.96 0.82 0.79 0.75
Endoplasmic Reticulum 0 0 0 0 0.07 0.14 0.12 0.24 0.17 0.22 0.14 0.16 0 0 0 0.06 – 0.06 0 0 0 0.1 0.13 0.14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.08 0.09 0.05 0 0 0 0 – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 12 11 23 24 13 27 0 0 0 0 – 0 0 0 0 0 0 1
Bud 0 0 0 0 0 1 0 1 0 0 0 0 0 1 0 0 – 0 0 1 3 0 2 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 – – – – – –
Cell Periphery 3 4 11 3 18 7 20 28 23 24 16 41 0 1 3 0 – 0 0 0 0 0 0 0
Cytoplasm 207 230 321 89 279 312 326 310 287 249 221 213 181 290 257 16 – 52 200 226 328 133 169 211
Endoplasmic Reticulum 2 3 10 4 23 53 48 110 65 80 43 53 6 12 9 1 – 3 2 3 4 16 29 40
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 1 0 0 0 2 15 10 10 8 14 0 0 0 0 – 0 0 0 0 1 0 3
Mitochondria 0 0 1 0 0 1 3 5 20 28 26 18 0 0 0 0 – 0 0 0 0 1 3 7
Nucleus 0 0 0 1 1 1 3 1 0 1 2 3 0 0 0 0 – 0 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 2 0 1 0 – 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 1 6 2 1 15 1 9 7 15 3 3 0 0 – 2 2 3 2 3 4 4
Unique Cell Count 210 232 330 96 308 370 389 451 385 372 300 330 191 303 266 16 52 206 238 342 164 216 284
Labelled Cell Count 212 237 346 98 327 378 415 498 429 425 336 385 192 307 270 17 57 206 238 342 164 216 284


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 58.7 61.5 46.3 41.0 37.2 29.2 26.9 26.6 23.2 21.7 20.1 20.8 57.7 58.7 54.0 70.6 – 56.7 51.2 52.8 60.3
Std Deviation (1e-4) 12.1 13.2 9.5 11.7 7.4 6.2 5.9 6.3 5.1 4.8 4.7 4.7 14.6 14.5 15.4 27.2 – 15.6 14.6 15.9 16.8
Intensity Change (Log2) – – – -0.17 -0.32 -0.66 -0.78 -0.8 -1.0 -1.09 -1.2 -1.15 0.32 0.34 0.22 0.61 – 0.29 0.14 0.19 0.38


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 3.2 0 4.5 4.7 3.8 5.3 0 0 0 0 – 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Cell Periphery 0 1.5 -1.2 1.2 1.8 1.7 1.9 1.2 4.3 0 -2.8 -1.8 0 – 0
Cytoplasm -2.1 -3.6 -5.8 -6.0 -10.0 -8.5 -10.3 -8.5 -10.7 -1.5 -1.1 -0.5 0.7 – 1.2
Endoplasmic Reticulum 0 2.5 5.2 4.6 8.2 6.0 7.3 5.1 5.7 0.1 0.6 0.2 0 – 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Golgi 0 0 0 0 2.9 2.5 2.5 0 3.4 0 0 0 0 – 0
Mitochondria 0 0 0 0 0 3.9 4.8 5.2 4.0 0 0 0 0 – 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0
Vacuole 0 0 0 0 2.6 0 1.9 0 3.2 0 0 0 0 – 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nip1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available