Standard name
Human Ortholog
Description Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.06 0.09 0 0 0 0 0 0 0 0.11 0.08 0.07 0 0.05 0.11 0 0 0 0.05 0.07 0.05
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.07 0.05 0 0 0 0 0 0 0 0.05 0.07 0.06 0.14 0.13 0.14 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.07 0 0.06 0.05 0 0 0 0 0.06 0.06 0.11 0.14 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0.07 0.05 0 0.05 0 0 0 0 0.05 0.08 0.11 0.14 0.15 0.12 0.09 0.05 0.05 0.08 0.1 0.08
Golgi 0.07 0.22 0.54 0.56 0.16 0.09 0.14 0 0 0 0 0.66 0.58 0.53 0.24 0.34 0.38 0.1 0.24 0.09 0.17 0.18 0.18
Mitochondria 0.86 0.78 0.72 0.35 0.62 0.8 0.72 0.81 0.74 0.93 0.82 0.26 0.16 0.1 0.21 0.14 0.08 0.64 0.5 0.7 0.54 0.48 0.53
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.05 0 0 0 0.05 0.06 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0.05 0.08 0.11 0.15 0.15 0.09 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0.06 0.06 0.05
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.13 0.1 0.21 0.2 0.12 0.08 0 0.12 0.06 0.08 0.23 0.26 0.25 0.35 0.18 0.12 0 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 5 15 5 5 3 0 1 1 1 38 30 28 2 5 11 5 4 1 11 31 28
Bud 4 0 0 2 4 8 5 1 0 4 3 3 3 4 1 6 3 0 1 0 3 5 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 3 2
Bud Site 0 0 0 0 0 4 0 1 0 0 0 0 0 0 0 0 1
Cell Periphery 0 2 6 9 4 2 0 0 1 2 0 19 26 26 16 14 14 0 0 0 0 0 2
Cytoplasm 6 4 1 9 11 8 9 5 6 2 3 13 23 26 12 15 18 0 1 0 0 0 0
Endoplasmic Reticulum 4 3 2 8 4 1 0 1 3 1 1 9 12 21 2 6 3 1 1 0 0 4 5
Endosome 11 3 3 13 8 8 8 3 2 0 3 18 32 47 15 16 12 25 6 2 20 44 41
Golgi 17 23 44 98 27 22 22 4 5 4 4 236 222 220 27 37 39 26 30 4 42 81 91
Mitochondria 225 81 59 61 101 201 113 81 116 150 102 94 62 40 23 15 8 173 62 34 134 222 275
Nucleus 2 0 0 0 0 0 1 2 4 1 1 1 1 0 3 0 0 0 0 0 1 1 1
Nuclear Periphery 12 2 1 2 8 14 9 5 4 9 5 0 7 4 0 2 1 7 1 1 4 7 7
Nucleolus 18 4 0 5 8 20 18 15 23 15 17 2 2 1 2 3 0 1 0 0 0 2 1
Peroxisomes 0 0 1 1 2 0 0 0 1 0 0 3 2 4 4 5 2 12 3 2 14 29 26
SpindlePole 3 0 0 0 0 3 3 2 3 0 1 3 8 1 8 3 3 1 0 0 3 7 7
Vac/Vac Membrane 50 14 8 37 33 31 13 4 19 9 10 82 101 104 39 20 12 5 8 1 9 15 16
Unique Cell Count 261 104 82 174 164 252 157 100 157 162 124 355 383 419 111 110 103 269 126 50 250 463 522
Labelled Cell Count 353 138 130 260 215 327 204 124 188 198 151 521 531 526 154 147 127 269 126 50 250 463 522


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 10.0 9.3 10.7 9.7 9.4 9.3 8.5 8.4 8.3 8.7 11.2 10.6 10.3 10.1 9.9 9.8 10.5 10.1 10.4
Std Deviation (1e-4) 1.3 2.2 2.0 1.7 1.7 1.9 1.7 1.8 1.9 1.8 2.0 1.7 1.5 1.5 2.0 2.6 2.5 1.9 1.8 1.8
Intensity Change (Log2)


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2505 3.7565 3.801 3.1048 3.3753 3.7051 3.8474 4.6844 4.3624 3.8201 3.9341 4.0436 3.9793 4.09 4.0317 4.184 4.2125 4.1247
Actin 0.0321 0.02 0.0237 0.0678 0.0536 0.0084 0.032 0.0188 0.0405 0.0226 0.0393 0.0162 0.0315 0.0334 0.0886 0.0127 0.0781 0.0267
Bud 0.0009 0.0025 0.0011 0.0005 0.0003 0.0002 0.0022 0.0038 0.0045 0.0054 0.0021 0.0072 0.0007 0.0026 0.0025 0.0035 0.0157 0.0045
Bud Neck 0.002 0.0008 0.0093 0.0062 0.0007 0.0006 0.0014 0.0008 0.0029 0.0008 0.002 0.0024 0.0009 0.0007 0.0013 0.0012 0.0007 0.0009
Bud Periphery 0.0019 0.0028 0.0017 0.0014 0.0004 0.0004 0.0037 0.0042 0.006 0.0106 0.0045 0.0087 0.0014 0.0032 0.003 0.0065 0.027 0.002
Bud Site 0.0065 0.0176 0.0087 0.0007 0.0014 0.0002 0.0107 0.0314 0.0144 0.0127 0.0038 0.0046 0.0018 0.018 0.0086 0.0066 0.0096 0.0009
Cell Periphery 0.0006 0.0008 0.0008 0.0003 0.0001 0.0001 0.0011 0.0009 0.0014 0.0013 0.0005 0.001 0.0004 0.0008 0.001 0.0006 0.001 0.0004
Cytoplasm 0.0063 0.0139 0.0197 0.0013 0.0081 0.0061 0.0189 0.0198 0.0234 0.0519 0.0224 0.0279 0.0029 0.0246 0.0094 0.0016 0.0041 0.0068
Cytoplasmic Foci 0.0337 0.0349 0.0313 0.0222 0.0359 0.0065 0.0648 0.0431 0.0416 0.0174 0.0511 0.0397 0.0397 0.0321 0.0234 0.0321 0.0185 0.0232
Eisosomes 0.0007 0.0003 0.0006 0.0007 0.0003 0.0002 0.0012 0.0005 0.0009 0.0006 0.0003 0.0003 0.0007 0.0004 0.0016 0.0004 0.0003 0.0003
Endoplasmic Reticulum 0.004 0.0028 0.0024 0.0013 0.0047 0.0057 0.0059 0.0072 0.0061 0.0014 0.0038 0.0012 0.0026 0.002 0.002 0.0005 0.0019 0.0038
Endosome 0.0915 0.1257 0.0769 0.047 0.1116 0.0439 0.1298 0.1006 0.0852 0.0486 0.161 0.0955 0.1102 0.0848 0.0681 0.0323 0.1234 0.0789
Golgi 0.0732 0.1215 0.0779 0.0594 0.1272 0.0763 0.0696 0.1241 0.0892 0.046 0.1812 0.1008 0.1567 0.1532 0.1492 0.0899 0.1313 0.1538
Lipid Particles 0.0653 0.0109 0.0124 0.0081 0.0441 0.0023 0.0464 0.0164 0.0093 0.0066 0.0139 0.0108 0.0294 0.011 0.005 0.0022 0.0011 0.0066
Mitochondria 0.5427 0.5933 0.6826 0.7592 0.434 0.8419 0.4576 0.574 0.6225 0.7436 0.484 0.6498 0.5393 0.5969 0.5932 0.7896 0.5747 0.672
None 0.0205 0.0008 0.0107 0.0004 0.0026 0.0003 0.0204 0.0085 0.0034 0.0005 0.0012 0.005 0.0247 0.0053 0.005 0.0003 0.0003 0.0053
Nuclear Periphery 0.0258 0.0018 0.0014 0.001 0.0906 0.0008 0.0071 0.0012 0.006 0.0007 0.0008 0.0005 0.0104 0.0009 0.0049 0.0001 0.0006 0.0016
Nucleolus 0.0088 0.0009 0.0003 0.0002 0.0011 0 0.0025 0.0007 0.0006 0.0003 0.0001 0.0011 0.0002 0.0004 0.0011 0.0002 0.0001 0.0003
Nucleus 0.0129 0.0009 0.0006 0.0005 0.0133 0.0004 0.008 0.001 0.0008 0.0006 0.0004 0.0015 0.0005 0.0011 0.0119 0.0002 0.0004 0.0004
Peroxisomes 0.0367 0.017 0.0277 0.0098 0.035 0.0015 0.0696 0.0284 0.0209 0.01 0.0177 0.011 0.025 0.0115 0.0071 0.0127 0.0029 0.0045
Punctate Nuclear 0.0169 0.0004 0.0017 0.0002 0.0293 0.0003 0.0221 0.0004 0.0106 0.0002 0.0007 0.0015 0.0004 0.0004 0.0008 0.0008 0.0001 0.0002
Vacuole 0.0093 0.0179 0.0049 0.0052 0.0018 0.0015 0.0168 0.01 0.0056 0.0137 0.0037 0.0074 0.0054 0.0106 0.0084 0.0027 0.0046 0.0028
Vacuole Periphery 0.008 0.0126 0.0036 0.0064 0.0038 0.0024 0.0083 0.0041 0.0042 0.0047 0.0056 0.0058 0.0151 0.0058 0.004 0.0034 0.0038 0.0043

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 29.9776 14.4297 14.5316 25.482 26.1606 31.1133 41.1644 40.7955 38.184 37.3307
Translational Efficiency 1.1198 1.8546 1.9411 1.0111 1.0453 1.5327 1.0082 0.9665 1.2674 1.1205

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency