Standard name | |
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Human Ortholog | |
Description | Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 3 | 6 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Bud | 1 | 0 | 2 | 0 | 0 | 0 | 4 | 3 | 1 | 1 | 1 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 7 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 1 | 0 | 3 | 7 | 13 | 14 | 9 | 12 | 15 | 7 | 8 | 2 | 1 | 1 | 19 | 10 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 461 | 142 | 313 | 318 | 492 | 520 | 499 | 427 | 398 | 411 | 226 | 353 | 281 | 327 | 344 | 305 | 328 | 276 | 430 | 150 | 292 | 307 | 431 | 396 |
Endoplasmic Reticulum | 2 | 1 | 2 | 2 | 22 | 9 | 13 | 52 | 21 | 35 | 10 | 19 | 4 | 3 | 1 | 7 | 8 | 6 | 3 | 0 | 1 | 3 | 3 | 3 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 5 | 10 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 3 | 1 | 2 |
Mitochondria | 1 | 1 | 0 | 0 | 0 | 3 | 10 | 1 | 8 | 6 | 10 | 4 | 0 | 0 | 0 | 2 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 2 |
Nucleus | 2 | 1 | 4 | 8 | 10 | 6 | 14 | 13 | 12 | 9 | 10 | 7 | 2 | 1 | 2 | 4 | 0 | 0 | 1 | 1 | 5 | 3 | 6 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 5 |
Vac/Vac Membrane | 2 | 1 | 1 | 5 | 8 | 28 | 47 | 12 | 5 | 22 | 10 | 21 | 1 | 11 | 1 | 3 | 1 | 1 | 0 | 1 | 2 | 0 | 5 | 4 |
Unique Cell Count | 466 | 145 | 316 | 325 | 520 | 563 | 583 | 501 | 440 | 486 | 258 | 402 | 285 | 337 | 347 | 311 | 333 | 279 | 450 | 160 | 309 | 328 | 466 | 448 |
Labelled Cell Count | 472 | 147 | 322 | 336 | 539 | 581 | 605 | 518 | 460 | 506 | 274 | 422 | 290 | 343 | 350 | 340 | 349 | 296 | 450 | 160 | 309 | 328 | 466 | 448 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 16.6 | 23.4 | 15.7 | 14.3 | 14.5 | 13.0 | 11.8 | 12.9 | 11.6 | 12.2 | 10.4 | 12.3 | 16.8 | 17.2 | 16.7 | 25.3 | 25.2 | 25.2 | 17.2 | 16.8 | 18.2 |
Std Deviation (1e-4) | 3.0 | 4.3 | 2.3 | 2.1 | 2.0 | 1.9 | 2.1 | 2.2 | 2.1 | 2.3 | 2.3 | 2.5 | 2.9 | 2.4 | 2.7 | 5.7 | 5.2 | 5.4 | 2.5 | 3.2 | 3.1 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.11 | -0.28 | -0.41 | -0.28 | -0.44 | -0.37 | -0.6 | -0.35 | 0.1 | 0.13 | 0.09 | 0.69 | 0.68 | 0.68 | 0.13 | 0.1 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 4.5 | 0 | 0 |
Cytoplasm | -1.2 | -3.3 | -4.3 | -6.5 | -6.6 | -4.9 | -6.8 | -5.7 | -5.8 | -0.5 | -1.8 | 0.1 | -1.0 | -0.6 | -0.2 |
Endoplasmic Reticulum | 0 | 3.0 | 0 | 1.8 | 5.5 | 3.3 | 4.3 | 2.7 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.1 | 0.7 | 0 | 1.2 | 1.3 | 1.4 | 0.6 | 2.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.7 | 4.9 | 2.3 | 0 | 3.5 | 0 | 3.8 | 0 | 2.8 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.0402 | 22.0077 | 19.3455 | 18.6511 | 18.4623 | 19.9629 | 15.7478 | 18.771 | 16.8542 | 15.0025 | 15.3849 | 17.2478 | 17.416 | 20.5625 | 18.8442 | 19.3788 | 17.442 | 19.1132 |
Actin | 0.0047 | 0.0004 | 0.0105 | 0.0029 | 0.0112 | 0.0006 | 0.014 | 0.0002 | 0.0104 | 0.0258 | 0.0013 | 0.0057 | 0.0075 | 0.0002 | 0.004 | 0.0001 | 0.0014 | 0.0027 |
Bud | 0.0005 | 0.0002 | 0.0002 | 0.0013 | 0.0003 | 0.0001 | 0.0012 | 0.0001 | 0.0004 | 0.0007 | 0.0001 | 0.0003 | 0.0007 | 0 | 0.0005 | 0.0001 | 0.0004 | 0.0001 |
Bud Neck | 0.0011 | 0.0003 | 0.0008 | 0.0007 | 0.004 | 0.0013 | 0.0009 | 0.0003 | 0.001 | 0.0027 | 0.0008 | 0.0057 | 0.0048 | 0.0002 | 0.0005 | 0.0009 | 0.0011 | 0.0036 |
Bud Periphery | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0007 | 0 | 0.0004 | 0.0009 | 0 | 0.0003 | 0.0005 | 0 | 0.0005 | 0 | 0.0002 | 0 |
Bud Site | 0.0021 | 0.0003 | 0.0005 | 0.0041 | 0.0006 | 0.0001 | 0.0021 | 0.0001 | 0.0008 | 0.0026 | 0.0003 | 0.0003 | 0.0064 | 0.0001 | 0.0013 | 0.0003 | 0.0003 | 0.0002 |
Cell Periphery | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.8718 | 0.9698 | 0.9041 | 0.9 | 0.8647 | 0.9396 | 0.8576 | 0.9689 | 0.8834 | 0.8583 | 0.872 | 0.9049 | 0.8737 | 0.9883 | 0.9068 | 0.934 | 0.8968 | 0.9362 |
Cytoplasmic Foci | 0.0227 | 0.0018 | 0.0128 | 0.0125 | 0.0363 | 0.0047 | 0.013 | 0.0005 | 0.0121 | 0.011 | 0.0098 | 0.0021 | 0.0231 | 0.0005 | 0.0103 | 0.004 | 0.0037 | 0.0031 |
Eisosomes | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.005 | 0.0007 | 0.0022 | 0.0012 | 0.0033 | 0.0017 | 0.0074 | 0.0009 | 0.006 | 0.0024 | 0.0064 | 0.0025 | 0.0035 | 0.0005 | 0.0037 | 0.0004 | 0.0033 | 0.0015 |
Endosome | 0.0314 | 0.0024 | 0.0101 | 0.012 | 0.0095 | 0.0055 | 0.0167 | 0.0002 | 0.0139 | 0.0101 | 0.0292 | 0.0051 | 0.0235 | 0.0001 | 0.0148 | 0.0086 | 0.0082 | 0.0007 |
Golgi | 0.0062 | 0.0004 | 0.003 | 0.0073 | 0.0175 | 0.0028 | 0.0046 | 0 | 0.0029 | 0.0138 | 0.001 | 0.0011 | 0.0056 | 0 | 0.0047 | 0.001 | 0.0018 | 0.0003 |
Lipid Particles | 0.0071 | 0 | 0.0087 | 0.009 | 0.0041 | 0.0021 | 0.0058 | 0 | 0.0047 | 0.0115 | 0.0039 | 0.0025 | 0.006 | 0 | 0.004 | 0.0103 | 0.0041 | 0.0001 |
Mitochondria | 0.0042 | 0.0006 | 0.0064 | 0.0013 | 0.0012 | 0.0031 | 0.0101 | 0.0001 | 0.0017 | 0.0066 | 0.0008 | 0.001 | 0.0021 | 0 | 0.0072 | 0.0002 | 0.0131 | 0.0001 |
None | 0.0027 | 0.0004 | 0.0015 | 0.0006 | 0.0007 | 0.0006 | 0.0044 | 0.0004 | 0.001 | 0.0008 | 0.0111 | 0.0026 | 0.0021 | 0.0003 | 0.0006 | 0.0004 | 0.0046 | 0.0005 |
Nuclear Periphery | 0.0086 | 0.0018 | 0.0047 | 0.0037 | 0.0037 | 0.0033 | 0.0079 | 0.0022 | 0.0059 | 0.0057 | 0.0113 | 0.0117 | 0.004 | 0.0008 | 0.0059 | 0.0029 | 0.0112 | 0.0029 |
Nucleolus | 0.001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0004 | 0.0004 | 0.0003 | 0 | 0.0002 | 0.0009 | 0.0007 | 0 |
Nucleus | 0.02 | 0.0193 | 0.023 | 0.0383 | 0.0262 | 0.0316 | 0.0415 | 0.0254 | 0.0469 | 0.0406 | 0.0405 | 0.049 | 0.0282 | 0.0086 | 0.0271 | 0.0313 | 0.0423 | 0.047 |
Peroxisomes | 0.0019 | 0.0001 | 0.0079 | 0.0018 | 0.0104 | 0.0008 | 0.0041 | 0 | 0.0023 | 0.0036 | 0.0003 | 0.0003 | 0.003 | 0 | 0.0022 | 0.0009 | 0.0007 | 0.0003 |
Punctate Nuclear | 0.0035 | 0.0004 | 0.0012 | 0.0013 | 0.0019 | 0.0013 | 0.0033 | 0.0004 | 0.0028 | 0.001 | 0.0034 | 0.003 | 0.0017 | 0.0002 | 0.0007 | 0.0006 | 0.0014 | 0.0005 |
Vacuole | 0.0041 | 0.0007 | 0.0011 | 0.0009 | 0.0037 | 0.0005 | 0.0029 | 0.0002 | 0.0027 | 0.001 | 0.0059 | 0.0007 | 0.0024 | 0.0001 | 0.0039 | 0.0029 | 0.0031 | 0.0002 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0002 | 0.0008 | 0 | 0.0006 | 0.0004 | 0.0014 | 0.0007 | 0.0005 | 0 | 0.0008 | 0.0002 | 0.0013 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 84.6139 | 104.9336 | 120.0423 | 114.3787 | 84.3402 | 71.3687 | 118.9185 | 105.6236 | 119.1469 | 110.6162 |
Translational Efficiency | 1.1898 | 1.2448 | 0.9793 | 1.0158 | 1.2933 | 1.5411 | 1.1268 | 1.3019 | 1.0709 | 1.0496 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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