Standard name
Human Ortholog
Description Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.98 0.95 0.92 0.86 0.85 0.9 0.85 0.88 0.88 0.99 0.97 0.99 0.98 0.98 0.99 0.96 0.94 0.95 0.94 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.1 0.05 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.08 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 2 4 1 3 6 0 5 0 0 0 0 0 0 0 0 0 1 1 1
Bud 1 0 2 0 0 0 4 3 1 1 1 5 0 0 0 0 0 0 0 2 0 1 0 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 1 0 3 7 13 14 9 12 15 7 8 2 1 1 19 10 9 0 0 0 0 0 0
Cytoplasm 461 142 313 318 492 520 499 427 398 411 226 353 281 327 344 305 328 276 430 150 292 307 431 396
Endoplasmic Reticulum 2 1 2 2 22 9 13 52 21 35 10 19 4 3 1 7 8 6 3 0 1 3 3 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 2 5 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 3 1 2
Mitochondria 1 1 0 0 0 3 10 1 8 6 10 4 0 0 0 2 1 2 2 0 2 0 0 2
Nucleus 2 1 4 8 10 6 14 13 12 9 10 7 2 1 2 4 0 0 1 1 5 3 6 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5
Vac/Vac Membrane 2 1 1 5 8 28 47 12 5 22 10 21 1 11 1 3 1 1 0 1 2 0 5 4
Unique Cell Count 466 145 316 325 520 563 583 501 440 486 258 402 285 337 347 311 333 279 450 160 309 328 466 448
Labelled Cell Count 472 147 322 336 539 581 605 518 460 506 274 422 290 343 350 340 349 296 450 160 309 328 466 448


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.6 23.4 15.7 14.3 14.5 13.0 11.8 12.9 11.6 12.2 10.4 12.3 16.8 17.2 16.7 25.3 25.2 25.2 17.2 16.8 18.2
Std Deviation (1e-4) 3.0 4.3 2.3 2.1 2.0 1.9 2.1 2.2 2.1 2.3 2.3 2.5 2.9 2.4 2.7 5.7 5.2 5.4 2.5 3.2 3.1
Intensity Change (Log2) -0.13 -0.11 -0.28 -0.41 -0.28 -0.44 -0.37 -0.6 -0.35 0.1 0.13 0.09 0.69 0.68 0.68 0.13 0.1 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.0 3.2 0 0 0 0 0 4.5 0 0
Cytoplasm -1.2 -3.3 -4.3 -6.5 -6.6 -4.9 -6.8 -5.7 -5.8 -0.5 -1.8 0.1 -1.0 -0.6 -0.2
Endoplasmic Reticulum 0 3.0 0 1.8 5.5 3.3 4.3 2.7 3.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.1 0.7 0 1.2 1.3 1.4 0.6 2.0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.7 4.9 2.3 0 3.5 0 3.8 0 2.8 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.0402 22.0077 19.3455 18.6511 18.4623 19.9629 15.7478 18.771 16.8542 15.0025 15.3849 17.2478 17.416 20.5625 18.8442 19.3788 17.442 19.1132
Actin 0.0047 0.0004 0.0105 0.0029 0.0112 0.0006 0.014 0.0002 0.0104 0.0258 0.0013 0.0057 0.0075 0.0002 0.004 0.0001 0.0014 0.0027
Bud 0.0005 0.0002 0.0002 0.0013 0.0003 0.0001 0.0012 0.0001 0.0004 0.0007 0.0001 0.0003 0.0007 0 0.0005 0.0001 0.0004 0.0001
Bud Neck 0.0011 0.0003 0.0008 0.0007 0.004 0.0013 0.0009 0.0003 0.001 0.0027 0.0008 0.0057 0.0048 0.0002 0.0005 0.0009 0.0011 0.0036
Bud Periphery 0.0005 0.0001 0.0002 0.0005 0.0002 0.0001 0.0007 0 0.0004 0.0009 0 0.0003 0.0005 0 0.0005 0 0.0002 0
Bud Site 0.0021 0.0003 0.0005 0.0041 0.0006 0.0001 0.0021 0.0001 0.0008 0.0026 0.0003 0.0003 0.0064 0.0001 0.0013 0.0003 0.0003 0.0002
Cell Periphery 0.0002 0 0.0001 0 0.0001 0 0.0003 0 0.0001 0.0004 0.0001 0 0.0002 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.8718 0.9698 0.9041 0.9 0.8647 0.9396 0.8576 0.9689 0.8834 0.8583 0.872 0.9049 0.8737 0.9883 0.9068 0.934 0.8968 0.9362
Cytoplasmic Foci 0.0227 0.0018 0.0128 0.0125 0.0363 0.0047 0.013 0.0005 0.0121 0.011 0.0098 0.0021 0.0231 0.0005 0.0103 0.004 0.0037 0.0031
Eisosomes 0 0 0.0001 0 0 0 0.0003 0 0.0001 0.0002 0 0.0001 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.005 0.0007 0.0022 0.0012 0.0033 0.0017 0.0074 0.0009 0.006 0.0024 0.0064 0.0025 0.0035 0.0005 0.0037 0.0004 0.0033 0.0015
Endosome 0.0314 0.0024 0.0101 0.012 0.0095 0.0055 0.0167 0.0002 0.0139 0.0101 0.0292 0.0051 0.0235 0.0001 0.0148 0.0086 0.0082 0.0007
Golgi 0.0062 0.0004 0.003 0.0073 0.0175 0.0028 0.0046 0 0.0029 0.0138 0.001 0.0011 0.0056 0 0.0047 0.001 0.0018 0.0003
Lipid Particles 0.0071 0 0.0087 0.009 0.0041 0.0021 0.0058 0 0.0047 0.0115 0.0039 0.0025 0.006 0 0.004 0.0103 0.0041 0.0001
Mitochondria 0.0042 0.0006 0.0064 0.0013 0.0012 0.0031 0.0101 0.0001 0.0017 0.0066 0.0008 0.001 0.0021 0 0.0072 0.0002 0.0131 0.0001
None 0.0027 0.0004 0.0015 0.0006 0.0007 0.0006 0.0044 0.0004 0.001 0.0008 0.0111 0.0026 0.0021 0.0003 0.0006 0.0004 0.0046 0.0005
Nuclear Periphery 0.0086 0.0018 0.0047 0.0037 0.0037 0.0033 0.0079 0.0022 0.0059 0.0057 0.0113 0.0117 0.004 0.0008 0.0059 0.0029 0.0112 0.0029
Nucleolus 0.001 0 0.0003 0.0001 0.0001 0.0001 0.0003 0 0.0002 0.0001 0.0004 0.0004 0.0003 0 0.0002 0.0009 0.0007 0
Nucleus 0.02 0.0193 0.023 0.0383 0.0262 0.0316 0.0415 0.0254 0.0469 0.0406 0.0405 0.049 0.0282 0.0086 0.0271 0.0313 0.0423 0.047
Peroxisomes 0.0019 0.0001 0.0079 0.0018 0.0104 0.0008 0.0041 0 0.0023 0.0036 0.0003 0.0003 0.003 0 0.0022 0.0009 0.0007 0.0003
Punctate Nuclear 0.0035 0.0004 0.0012 0.0013 0.0019 0.0013 0.0033 0.0004 0.0028 0.001 0.0034 0.003 0.0017 0.0002 0.0007 0.0006 0.0014 0.0005
Vacuole 0.0041 0.0007 0.0011 0.0009 0.0037 0.0005 0.0029 0.0002 0.0027 0.001 0.0059 0.0007 0.0024 0.0001 0.0039 0.0029 0.0031 0.0002
Vacuole Periphery 0.0009 0.0002 0.0007 0.0003 0.0003 0.0002 0.0008 0 0.0006 0.0004 0.0014 0.0007 0.0005 0 0.0008 0.0002 0.0013 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 84.6139 104.9336 120.0423 114.3787 84.3402 71.3687 118.9185 105.6236 119.1469 110.6162
Translational Efficiency 1.1898 1.2448 0.9793 1.0158 1.2933 1.5411 1.1268 1.3019 1.0709 1.0496

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mck1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available