Standard name
Description Dual-specificity ser/thr and tyrosine protein kinase; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent protein degradation (Rcn1p and Cdc6p), inhibition of protein kinase A, transcriptional regulation, inhibition of RNA pol III, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division; MCK1 has a paralog, YGK3, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.99 0.98 0.95 0.92 0.86 0.85 0.9 0.85 0.88 0.88 0.99 0.97 0.99 0.98 0.98 0.99 0.96 0.94 0.95 0.94 0.93 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.1 0.05 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0.08 0 0 0.05 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 2 4 1 3 6 0 5 0 0 0 0 0 0 0 0 0 1 1 1
Bud 1 0 2 0 0 0 4 3 1 1 1 5 0 0 0 0 0 0 0 2 0 1 0 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 1 0 3 7 13 14 9 12 15 7 8 2 1 1 19 10 9 0 0 0 0 0 0
Cytoplasm 461 142 313 318 492 520 499 427 398 411 226 353 281 327 344 305 328 276 430 150 292 307 431 396
Endoplasmic Reticulum 2 1 2 2 22 9 13 52 21 35 10 19 4 3 1 7 8 6 3 0 1 3 3 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 0 0 2 5 10
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 3 1 2
Mitochondria 1 1 0 0 0 3 10 1 8 6 10 4 0 0 0 2 1 2 2 0 2 0 0 2
Nucleus 2 1 4 8 10 6 14 13 12 9 10 7 2 1 2 4 0 0 1 1 5 3 6 4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 5
Vac/Vac Membrane 2 1 1 5 8 28 47 12 5 22 10 21 1 11 1 3 1 1 0 1 2 0 5 4
Unique Cell Count 466 145 316 325 520 563 583 501 440 486 258 402 285 337 347 311 333 279 450 160 309 328 466 448
Labelled Cell Count 472 147 322 336 539 581 605 518 460 506 274 422 290 343 350 340 349 296 450 160 309 328 466 448


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.6 23.4 15.7 14.3 14.5 13.0 11.8 12.9 11.6 12.2 10.4 12.3 16.8 17.2 16.7 25.3 25.2 25.2 17.2 16.8 18.2
Std Deviation (1e-4) 3.0 4.3 2.3 2.1 2.0 1.9 2.1 2.2 2.1 2.3 2.3 2.5 2.9 2.4 2.7 5.7 5.2 5.4 2.5 3.2 3.1
Intensity Change (Log2) -0.13 -0.11 -0.28 -0.41 -0.28 -0.44 -0.37 -0.6 -0.35 0.1 0.13 0.09 0.69 0.68 0.68 0.13 0.1 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 3.0 3.2 0 0 0 0 0 4.5 0 0
Cytoplasm -1.2 -3.3 -4.3 -6.5 -6.6 -4.9 -6.8 -5.7 -5.8 -0.5 -1.8 0.1 -1.0 -0.6 -0.2
Endoplasmic Reticulum 0 3.0 0 1.8 5.5 3.3 4.3 2.7 3.2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.1 0.7 0 1.2 1.3 1.4 0.6 2.0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.7 4.9 2.3 0 3.5 0 3.8 0 2.8 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mck1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available