Standard name
Description Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.06 0.06 0 0.06 0.07 0 0 0 0 0.06 0.09 0.12 0.06 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.05 0.11 0.15 0.22 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.98 0.94 0.91 0.93 0.88 0.9 0.83 0.78 0.86 0.81 0.84 0.93 0.87 0.83 0.88 0.92 0.85 0.94 0.96 0.93 0.85 0.71 0.63
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.11 0.1 0.1 0.28 0.26 0.27 0.39 0 0 0 0 0 0.06 0 0 0 0 0.08 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0.09 0.17 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 2 5 1 1 6 1 4 0 0 0 0 0 2
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 5 6 5 9 5 11 9 3 4 4 1 27 49 85 28 6 14 0 0 1 0 2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 2 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 1 3 5 2 1 1 1 0 0 1 5 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 2 3 2
Mitochondria 2 1 0 0 0 3 9 6 23 19 34 9 1 2 1 3 2 9 2 1 0 0 7 10
Nucleus 269 249 108 72 134 102 175 104 168 113 126 126 446 489 589 412 243 276 260 245 106 305 418 355
Nuclear Periphery 0 0 0 0 1 0 3 1 2 0 1 1 0 1 0 1 3 4 0 0 0 0 3 10
Nucleolus 8 3 1 1 2 13 19 13 61 34 42 58 5 12 14 8 7 20 4 0 0 13 44 65
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 0 0 0 0 0 1 3 19 1 0 1 0 0 0 0 2 2
Vac/Vac Membrane 2 0 1 1 2 0 0 5 1 0 0 1 5 6 4 23 6 8 5 3 2 32 97 108
Unique Cell Count 281 253 115 79 144 116 195 126 215 131 155 150 482 559 706 469 263 324 276 255 114 357 589 567
Labelled Cell Count 284 258 116 79 148 127 223 140 260 176 208 201 486 565 721 483 272 338 276 255 114 357 589 567


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 12.8 9.9 10.6 10.1 7.6 8.1 8.7 7.0 6.5 6.5 6.8 10.5 11.2 11.3 15.6 13.5 14.6 11.3 11.2 11.5
Std Deviation (1e-4) 1.9 3.0 2.4 3.7 2.5 1.7 2.2 3.5 1.6 1.7 1.8 1.7 3.0 3.2 3.7 4.7 4.0 4.6 2.3 2.4 2.3
Intensity Change (Log2) 0.11 0.03 -0.37 -0.29 -0.18 -0.49 -0.6 -0.61 -0.53 0.09 0.18 0.2 0.66 0.45 0.56 0.19 0.18 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.4 -0.3 0.2 0.6 0 0 0 0 0.2 1.3 2.2 0.3 0 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.6 4.3 5.4 0 0 0 0 0 0 0
Nucleus -0.7 -0.3 -1.6 -1.3 -2.7 -3.7 -2.0 -3.0 -2.5 -0.5 -2.0 -2.9 -1.9 -0.5 -2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 3.3 3.1 3.1 6.1 5.6 5.8 7.3 0 0 0 0 0 2.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rfc3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available