Standard name | |
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Description | Multifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 10 | 4 | 22 | 14 | 16 | 1 | 3 | 7 | 2 | 1 | 2 | 6 | 6 | 6 | 3 | – | 10 | 3 | 14 | 5 | 7 | 21 | 30 | 23 |
Bud | 5 | 4 | 9 | 4 | 8 | 5 | 13 | 15 | 11 | 19 | 8 | 11 | 4 | 2 | 5 | – | 0 | 0 | 3 | 2 | 2 | 6 | 10 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 3 | 1 | 5 | 6 | 6 | 8 |
Bud Site | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 4 | 3 | 2 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 6 | 0 | 6 | 6 | 4 | 3 | 1 | 1 | 1 | 2 | 0 | 0 | 5 | 3 | 3 | – | 14 | 10 | 0 | 0 | 0 | 1 | 2 | 0 |
Cytoplasm | 102 | 51 | 125 | 122 | 154 | 80 | 80 | 67 | 54 | 53 | 30 | 33 | 233 | 193 | 229 | – | 156 | 116 | 44 | 30 | 66 | 35 | 36 | 27 |
Endoplasmic Reticulum | 21 | 4 | 5 | 8 | 15 | 5 | 10 | 3 | 7 | 2 | 2 | 6 | 23 | 11 | 15 | – | 7 | 3 | 12 | 1 | 5 | 32 | 20 | 27 |
Endosome | 39 | 8 | 19 | 14 | 50 | 55 | 37 | 39 | 22 | 14 | 5 | 17 | 21 | 14 | 19 | – | 4 | 9 | 84 | 36 | 44 | 41 | 71 | 62 |
Golgi | 13 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 3 | 5 | – | 24 | 6 | 26 | 6 | 6 | 11 | 30 | 18 |
Mitochondria | 23 | 26 | 4 | 17 | 20 | 61 | 124 | 70 | 176 | 232 | 153 | 184 | 22 | 11 | 16 | – | 2 | 1 | 41 | 43 | 112 | 57 | 88 | 74 |
Nucleus | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 5 | 5 | 1 | 2 | 1 | 0 | 1 | – | 2 | 1 | 0 | 0 | 2 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 3 | 0 | 1 | 2 | 2 | 2 |
Nucleolus | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 3 | 2 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
SpindlePole | 2 | 1 | 4 | 0 | 6 | 3 | 6 | 12 | 5 | 10 | 6 | 13 | 0 | 0 | 0 | – | 4 | 2 | 7 | 3 | 3 | 3 | 15 | 10 |
Vac/Vac Membrane | 10 | 11 | 33 | 18 | 47 | 58 | 41 | 50 | 46 | 47 | 22 | 22 | 26 | 37 | 32 | – | 25 | 24 | 13 | 23 | 27 | 25 | 24 | 18 |
Unique Cell Count | 199 | 95 | 204 | 184 | 286 | 230 | 271 | 215 | 270 | 321 | 195 | 243 | 326 | 256 | 301 | 219 | 153 | 264 | 160 | 295 | 254 | 356 | 293 | |
Labelled Cell Count | 235 | 110 | 228 | 205 | 322 | 277 | 319 | 270 | 333 | 392 | 235 | 301 | 348 | 280 | 328 | 248 | 175 | 264 | 160 | 295 | 254 | 356 | 293 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 6.3 | 7.5 | 7.6 | 7.4 | 6.5 | 6.0 | 6.4 | 5.6 | 5.1 | 4.8 | 4.9 | 8.2 | 7.9 | 7.7 | – | 13.9 | 13.3 | 7.5 | 7.6 | 7.7 |
Std Deviation (1e-4) | 1.0 | 1.2 | 1.4 | 1.3 | 1.1 | 1.4 | 1.4 | 1.7 | 1.4 | 1.5 | 1.2 | 1.0 | 1.3 | 1.2 | 1.3 | – | 2.8 | 2.6 | 1.3 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.01 | -0.02 | -0.2 | -0.32 | -0.24 | -0.44 | -0.57 | -0.63 | -0.61 | 0.12 | 0.07 | 0.03 | – | 0.88 | 0.83 | 0.0 | 0.02 | 0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -1.1 | -2.1 | -4.8 | -4.7 | -3.0 | -4.9 | -5.7 | 0 | -3.6 | -4.5 | -3.8 | -5.0 | – | -2.4 | -3.2 |
Bud | -1.2 | -1.0 | -1.3 | 0.2 | 1.1 | -0.2 | 0.7 | 0 | 0.1 | -2.3 | 0 | -1.8 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1.7 | 1.6 |
Cytoplasm | 1.0 | -1.6 | -5.5 | -6.9 | -6.2 | -9.2 | -10.6 | -9.4 | -10.5 | 2.4 | 3.3 | 3.6 | – | 2.2 | 2.9 |
Endoplasmic Reticulum | 1.0 | 1.5 | 0 | 0.8 | 0 | 0.1 | 0 | 0 | 0 | 2.3 | 1.1 | 1.4 | – | 0.5 | 0 |
Endosome | -0.6 | 2.6 | 4.0 | 1.5 | 2.6 | -0.4 | -2.3 | 0 | -0.9 | -1.2 | -1.6 | -1.3 | – | -3.4 | -1.2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 4.6 | 0 |
Mitochondria | 3.2 | 2.5 | 7.2 | 10.6 | 8.2 | 14.0 | 15.8 | 0 | 15.7 | 2.5 | 1.4 | 1.9 | – | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1.9 | 0 | 0.8 | 0 | 1.9 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | -1.9 | 0.1 | 2.3 | -0.3 | 1.8 | 0.2 | -0.5 | -1.4 | -2.3 | -2.9 | -0.5 | -1.8 | – | -1.4 | -0.1 |