Standard name
Description Multifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0.11 0.08 0.06 0 0 0 0 0 0 0 0 0 0 – 0.05 0 0.05 0 0 0.09 0.09 0.08
Bud 0 0 0 0 0 0 0.05 0.07 0 0.06 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.51 0.54 0.61 0.66 0.54 0.35 0.3 0.31 0.2 0.17 0.15 0.14 0.71 0.75 0.76 – 0.71 0.76 0.17 0.19 0.23 0.14 0.1 0.09
Endoplasmic Reticulum 0.11 0 0 0 0.05 0 0 0 0 0 0 0 0.07 0 0.05 – 0 0 0.05 0 0 0.13 0.06 0.09
Endosome 0.2 0.08 0.09 0.08 0.17 0.24 0.14 0.18 0.08 0 0 0.07 0.06 0.05 0.06 – 0 0.06 0.32 0.23 0.15 0.16 0.2 0.21
Golgi 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.11 0 0.1 0 0 0.05 0.09 0.06
Mitochondria 0.12 0.27 0 0.09 0.07 0.27 0.46 0.33 0.65 0.72 0.78 0.76 0.07 0 0.05 – 0 0 0.16 0.27 0.38 0.23 0.25 0.25
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0.06 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.12 0.16 0.1 0.16 0.25 0.15 0.23 0.17 0.15 0.11 0.09 0.08 0.14 0.11 – 0.11 0.16 0.05 0.15 0.09 0.1 0.07 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 4 22 14 16 1 3 7 2 1 2 6 6 6 3 – 10 3 14 5 7 21 30 23
Bud 5 4 9 4 8 5 13 15 11 19 8 11 4 2 5 – 0 0 3 2 2 6 10 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – 0 0 3 1 5 6 6 8
Bud Site 1 0 0 0 0 0 2 0 2 4 3 2 0 0 0 – 0 0 – – – – – –
Cell Periphery 6 0 6 6 4 3 1 1 1 2 0 0 5 3 3 – 14 10 0 0 0 1 2 0
Cytoplasm 102 51 125 122 154 80 80 67 54 53 30 33 233 193 229 – 156 116 44 30 66 35 36 27
Endoplasmic Reticulum 21 4 5 8 15 5 10 3 7 2 2 6 23 11 15 – 7 3 12 1 5 32 20 27
Endosome 39 8 19 14 50 55 37 39 22 14 5 17 21 14 19 – 4 9 84 36 44 41 71 62
Golgi 13 0 1 1 2 0 1 0 0 0 0 2 3 3 5 – 24 6 26 6 6 11 30 18
Mitochondria 23 26 4 17 20 61 124 70 176 232 153 184 22 11 16 – 2 1 41 43 112 57 88 74
Nucleus 1 0 0 1 0 1 1 4 5 5 1 2 1 0 1 – 2 1 0 0 2 0 1 1
Nuclear Periphery 0 0 0 0 0 2 0 0 1 2 0 0 2 0 0 – 0 0 3 0 1 2 2 2
Nucleolus 2 1 0 0 0 1 0 1 1 1 3 2 1 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 2 0 1 0 0 0 0 1 0 0 – 0 0 0 0 0 1 2 1
SpindlePole 2 1 4 0 6 3 6 12 5 10 6 13 0 0 0 – 4 2 7 3 3 3 15 10
Vac/Vac Membrane 10 11 33 18 47 58 41 50 46 47 22 22 26 37 32 – 25 24 13 23 27 25 24 18
Unique Cell Count 199 95 204 184 286 230 271 215 270 321 195 243 326 256 301 219 153 264 160 295 254 356 293
Labelled Cell Count 235 110 228 205 322 277 319 270 333 392 235 301 348 280 328 248 175 264 160 295 254 356 293


Cytoplasm, Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 6.3 7.5 7.6 7.4 6.5 6.0 6.4 5.6 5.1 4.8 4.9 8.2 7.9 7.7 – 13.9 13.3 7.5 7.6 7.7
Std Deviation (1e-4) 1.0 1.2 1.4 1.3 1.1 1.4 1.4 1.7 1.4 1.5 1.2 1.0 1.3 1.2 1.3 – 2.8 2.6 1.3 1.6 1.4
Intensity Change (Log2) – – – 0.01 -0.02 -0.2 -0.32 -0.24 -0.44 -0.57 -0.63 -0.61 0.12 0.07 0.03 – 0.88 0.83 0.0 0.02 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.1 -2.1 -4.8 -4.7 -3.0 -4.9 -5.7 0 -3.6 -4.5 -3.8 -5.0 – -2.4 -3.2
Bud -1.2 -1.0 -1.3 0.2 1.1 -0.2 0.7 0 0.1 -2.3 0 -1.8 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0.2 0 0 0 0 0 0 0 0 0 0 0 – 1.7 1.6
Cytoplasm 1.0 -1.6 -5.5 -6.9 -6.2 -9.2 -10.6 -9.4 -10.5 2.4 3.3 3.6 – 2.2 2.9
Endoplasmic Reticulum 1.0 1.5 0 0.8 0 0.1 0 0 0 2.3 1.1 1.4 – 0.5 0
Endosome -0.6 2.6 4.0 1.5 2.6 -0.4 -2.3 0 -0.9 -1.2 -1.6 -1.3 – -3.4 -1.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 4.6 0
Mitochondria 3.2 2.5 7.2 10.6 8.2 14.0 15.8 0 15.7 2.5 1.4 1.9 – 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 1.9 0 0.8 0 1.9 0 0 0 – 0 0
Vacuole -1.9 0.1 2.3 -0.3 1.8 0.2 -0.5 -1.4 -2.3 -2.9 -0.5 -1.8 – -1.4 -0.1