Standard name
Description RNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.06 0 0.05 0 0 0 0 0 0.13 0.1 0.19 0.07 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.97 0.95 0.92 0.89 0.85 0.82 0.76 0.74 0.82 0.75 0.7 0.73 0.81 0.8 0.68 0.81 0.79 0.82 0.93 0.92 0.87 0.74 0.76 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.16 0.1 0.16 0.18 0.28 0.31 0.38 0.46 0.43 0.55 0.48 0.13 0.15 0.16 0.11 0.13 0.09 0.05 0 0.05 0.13 0.11 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.1 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Bud Neck 0 0 0 0 0 1 0 3 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 2 0 2 5 0 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1 4 11 11 10 14 15 5 5 9 7 24 18 49 22 20 18 0 0 0 0 1 0
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 3 0 2 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 1 0 0 1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Mitochondria 1 0 1 0 0 4 4 5 9 5 19 7 1 2 0 9 3 4 0 0 1 0 0 1
Nucleus 264 182 149 195 163 227 210 265 228 217 200 221 152 149 177 254 187 228 253 187 153 60 116 116
Nuclear Periphery 0 0 0 2 0 0 4 0 0 0 1 0 0 0 1 0 1 0 0 0 1 0 0 0
Nucleolus 49 31 17 35 35 78 85 136 128 126 159 146 25 27 41 33 31 26 12 7 9 10 16 27
Peroxisomes 1 0 1 2 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 1 1 0 0 0 0 0 0 2 8 0 0 1 0 0 0 0 0 0
Vac/Vac Membrane 0 3 0 4 4 3 1 3 0 3 1 1 3 2 6 9 8 11 4 5 6 5 15 34
Unique Cell Count 271 192 162 220 191 278 275 358 279 291 287 302 187 186 259 314 237 278 274 204 176 82 154 190
Labelled Cell Count 315 217 173 249 215 324 321 427 373 362 390 391 205 201 286 331 252 291 274 204 176 82 154 190


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 22.1 28.5 20.5 19.7 21.5 18.6 17.9 17.8 15.9 15.4 14.7 15.2 29.0 26.8 29.4 31.7 32.1 30.2 18.5 22.7 25.9
Std Deviation (1e-4) 4.8 8.6 6.4 7.0 9.9 6.2 7.2 6.3 5.6 5.3 6.4 5.7 16.7 13.4 19.9 16.2 15.9 14.0 8.1 11.5 14.3
Intensity Change (Log2) – – – -0.06 0.06 -0.14 -0.2 -0.21 -0.37 -0.41 -0.48 -0.44 0.5 0.38 0.52 0.63 0.65 0.55 -0.15 0.14 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.5 0.7 1.3 1.0 0 0 0 0 3.6 2.8 5.0 2.1 2.5 1.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.0 0 0 0 0 0 0 0
Nucleus -1.1 -1.9 -3.0 -4.1 -4.7 -2.9 -4.5 -5.5 -4.8 -2.9 -3.1 -5.6 -3.2 -3.5 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.5 2.1 4.3 4.9 6.4 7.6 7.2 9.4 8.1 0.8 1.1 1.5 0 0.8 -0.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0