Standard name
Description Essential component of the TIM23 complex; with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core Translocase of the Inner Mitochondrial membrane (TIM23 complex)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.13 0.13 0.14 0.17 0.18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.96 0.97 0.91 0.91 0.84 0.78 0.66 0.72 0.7 0.71 0.69 0.93 0.93 0.92 0.97 0.97 0.95 0.82 0.78 0.72 0.78 0.69 0.72
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.14 0.11 0.17 0.09 0.2 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.06 0.05 0.06 0.1 0.21 0.15 0.19 0.08 0.12 0.09 0.05 0.09 0 0.08 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 2 1 3 2 7 1 1 0 5 2 0 0 0 0 1 0 0 0 0
Bud 1 0 0 2 9 10 7 4 7 13 7 10 1 7 1 0 0 2 26 25 14 31 66 59
Bud Neck 0 1 0 0 2 0 0 0 0 0 0 0 0 0 1 0 0 0 0 3 2 1 1 2
Bud Site 1 0 0 0 3 1 1 1 0 3 1 1 0 0 1 0 0 0
Cell Periphery 4 0 0 1 2 4 5 7 4 11 5 6 1 2 0 2 3 2 0 1 0 0 0 0
Cytoplasm 224 188 92 290 420 346 337 309 315 379 236 328 201 534 517 190 140 163 192 157 76 177 269 234
Endoplasmic Reticulum 1 0 0 1 0 1 13 12 7 11 8 9 0 2 6 7 3 5 0 1 1 0 9 2
Endosome 0 2 1 1 5 0 1 0 0 2 2 0 0 1 1 0 3 2 0 0 0 2 1 0
Golgi 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Mitochondria 1 0 3 4 16 53 62 50 75 51 65 119 1 1 0 5 2 1 1 1 1 0 4 0
Nucleus 2 1 0 4 3 6 7 12 7 10 6 6 0 1 2 0 0 1 2 2 3 3 6 8
Nuclear Periphery 0 0 0 1 0 2 2 4 4 3 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 1 5 1 2 2 3 0 1 4 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
SpindlePole 3 1 0 0 3 2 2 3 0 3 2 1 0 5 7 0 0 0 3 1 0 1 3 1
Vac/Vac Membrane 8 3 0 20 23 25 41 96 64 101 28 59 19 29 48 5 11 6 3 3 2 4 17 11
Unique Cell Count 236 195 95 318 462 411 431 465 436 541 332 475 216 572 563 195 145 171 234 202 107 229 391 327
Labelled Cell Count 245 196 96 326 487 452 480 506 486 598 364 545 223 588 590 210 162 183 234 202 107 229 391 327


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.2 28.6 19.5 19.8 18.3 13.3 13.0 13.5 13.1 13.7 13.8 12.8 24.8 26.7 27.9 9.4 9.5 10.3 33.0 38.3 39.9
Std Deviation (1e-4) 20.3 24.1 15.5 17.2 14.5 9.8 10.2 10.0 9.7 9.2 10.2 9.8 19.7 23.9 25.0 7.8 12.5 9.7 28.6 30.7 35.4
Intensity Change (Log2) 0.02 -0.09 -0.55 -0.58 -0.53 -0.57 -0.51 -0.5 -0.61 0.35 0.45 0.52 -1.05 -1.03 -0.93 0.76 0.97 1.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.8 -1.9 -3.3 -4.2 -6.0 -5.1 -5.5 -5.2 -5.6 -1.3 -1.3 -1.7 0.3 -0.1 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 2.7 3.0 2.3 3.5 2.0 3.9 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 4.9 4.0 4.6 2.9 3.6 3.0 0 3.0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tim17-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available