Standard name
Description Targeting subunit for Glc7p protein phosphatase; localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p; phosphorylation by Slt2p and Kss1p involved in regulating Bni4p in septum assembly
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.16 0.21 0.21 0.18 0.19 0.07 0.06 0.05 0 0 0 0 0.17 0.07 0.05 0.2 0.2 0.12 0.1 0.1 0.06 0.21 0.23 0.23
Bud 0.16 0.11 0.07 0.13 0.12 0.13 0.08 0.09 0.1 0.1 0.1 0.06 0.11 0.07 0.07 0.05 0.06 0 0.15 0.22 0.14 0.12 0.11 0.07
Bud Neck 0.05 0.06 0.11 0.06 0.06 0 0.05 0.06 0 0 0 0 0 0 0 0 0.06 0 0.41 0.33 0.37 0.28 0.26 0.26
Bud Site 0.09 0.11 0.08 0.1 0.12 0.11 0.05 0 0.05 0 0 0 0.11 0 0 0.05 0.1 0 – – – – – –
Cell Periphery 0.05 0.11 0.06 0.08 0 0 0 0 0 0 0 0 0.11 0.1 0.13 0.08 0.07 0.08 0 0 0 0.13 0.13 0.15
Cytoplasm 0.51 0.53 0.56 0.56 0.55 0.61 0.68 0.67 0.61 0.62 0.64 0.67 0.58 0.56 0.68 0.38 0.37 0.54 0.19 0.19 0.19 0.1 0.11 0.15
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.05 0 0
Endosome 0 0.05 0 0 0 0 0 0 0 0 0 0 0.07 0.19 0.14 0.05 0.07 0.06 0 0.06 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.07 0 0 0 0.1 0.13 0.08 0.33 0.37 0.38 0.24 0.05 0 0 0.05 0 0 0 0 0 0.06 0.05 0.05
Nucleus 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.14 0.1 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0 0.15 0.1 0.14 0.09 0.07 0.09 0 0 0 0.06 0 0.08 0 0.19 0.23 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0.08 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 36 34 41 27 33 13 15 15 1 2 3 2 45 27 20 38 42 28 29 22 16 57 78 75
Bud 35 17 14 19 21 25 20 29 22 25 21 13 30 27 28 10 12 9 43 46 36 32 37 22
Bud Neck 11 9 22 9 11 7 13 20 8 7 4 4 3 1 0 4 12 9 116 71 98 78 89 85
Bud Site 20 18 15 15 20 21 14 14 11 6 3 6 28 14 2 10 21 8 – – – – – –
Cell Periphery 12 17 11 11 4 7 1 3 5 3 1 4 30 40 56 15 14 19 5 4 7 37 45 48
Cytoplasm 113 85 107 82 94 119 179 211 139 155 128 142 152 224 282 72 77 121 53 39 48 27 36 50
Endoplasmic Reticulum 9 3 3 2 3 2 5 1 1 0 3 1 1 1 10 7 4 11 4 2 2 14 13 9
Endosome 7 8 0 0 0 3 2 3 0 0 2 0 18 76 60 10 14 13 8 11 3 1 1 3
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 5 2 1 2 2 1 0 1 0
Mitochondria 15 11 1 6 6 19 33 24 76 91 75 51 13 2 3 10 9 7 4 3 10 15 16 16
Nucleus 4 1 6 3 9 9 11 12 7 10 2 9 1 0 2 10 10 3 1 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0 3 0 1 0 0 2
Nucleolus 2 2 0 1 0 0 1 0 1 2 1 1 0 0 0 2 6 2 0 0 1 0 0 0
Peroxisomes 6 2 0 1 1 4 2 2 0 0 0 1 19 54 41 3 6 10 1 0 1 0 1 0
SpindlePole 12 3 28 15 24 17 18 29 8 9 5 12 11 31 10 37 48 30 5 3 11 0 0 1
Vac/Vac Membrane 4 2 3 0 0 1 0 4 0 3 1 0 1 3 2 14 10 18 4 3 14 8 9 5
Unique Cell Count 221 160 192 146 170 195 263 314 228 248 200 213 261 397 416 190 209 225 286 214 263 281 338 329
Labelled Cell Count 286 213 251 191 226 247 314 367 279 314 249 246 352 500 517 247 288 289 286 214 263 281 338 329


Bud Neck, Cell Periphery, Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 6.3 6.4 6.0 6.2 5.1 5.2 5.3 4.3 4.0 4.1 4.5 5.8 6.1 6.0 8.7 9.6 8.2 5.6 5.5 5.6
Std Deviation (1e-4) 0.8 1.0 1.3 1.8 1.5 1.8 1.5 1.6 1.3 1.2 1.5 1.7 1.1 1.2 1.4 1.6 6.8 1.5 1.4 1.7 1.6
Intensity Change (Log2) – – – -0.1 -0.04 -0.33 -0.31 -0.28 -0.59 -0.68 -0.66 -0.51 -0.15 -0.08 -0.1 0.44 0.57 0.35 -0.18 -0.23 -0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.6 -0.5 -4.2 -5.0 -5.8 0 0 0 0 -1.1 -5.2 -6.3 -0.3 -0.3 -2.4
Bud 1.8 1.6 1.8 0.1 0.8 0 0 0 0 1.5 -0.2 -0.3 -0.8 -0.6 -1.5
Bud Neck -1.7 -1.6 -2.9 -2.6 -2.0 0 0 0 0 -4.7 -6.6 -7.0 -3.6 -2.1 -2.9
Bud Site 0.8 1.3 1.0 -1.1 -1.6 0 0 0 0 1.0 -2.3 -5.1 -1.0 0.8 -1.9
Cell Periphery 0.7 -1.6 -1.0 -3.5 -3.2 0 0 0 0 2.1 1.8 2.8 0.8 0.4 1.1
Cytoplasm 0.1 -0.1 1.1 2.7 2.6 1.1 1.4 1.7 2.3 0.5 0.2 2.9 -3.5 -3.8 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1.9
Endosome 0 0 0 0 0 0 0 0 0 3.7 6.5 5.5 0 3.7 3.4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 4.8 3.6 0 0 0 0 2.7 0 0 2.8 0 0
Nucleus 0 1.0 0.8 0.6 0.4 0 0.5 0 0.6 0 0 0 1.0 0.8 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 3.8 5.4 4.5 0 0 0
SpindlePole -1.2 -0.1 -1.8 -2.7 -1.8 0 0 0 0 -3.9 -2.6 -5.8 1.3 2.1 -0.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 2.8 1.8 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.6565 3.9355 3.6559 3.1748 3.4314 3.4276 1.2796 2.3704 2.2282 1.9519 1.4818 2.1146 1.2254 2.1412 2.1748 1.4705 1.3823 2.5384
Actin 0.1207 0.0321 0.0298 0.0032 0.0698 0.0329 0.1424 0.0458 0.0644 0.1585 0.0113 0.0652 0.0906 0.0312 0.0335 0.0477 0.0757 0.0916
Bud 0.0057 0.0023 0.0017 0.0005 0.0007 0.0029 0.0094 0.0069 0.0046 0.0048 0.0015 0.0036 0.0358 0.0547 0.0252 0.0166 0.0712 0.0577
Bud Neck 0.1428 0.05 0.5292 0.9268 0.555 0.6988 0.1413 0.0374 0.5616 0.5328 0.6353 0.6858 0.0606 0.0256 0.3872 0.6967 0.3381 0.3335
Bud Periphery 0.0147 0.0032 0.0022 0.0011 0.0012 0.0081 0.0142 0.0079 0.0056 0.0219 0.0033 0.0092 0.0531 0.0371 0.0282 0.0271 0.1046 0.0791
Bud Site 0.4052 0.7512 0.3862 0.036 0.01 0.0227 0.4484 0.6953 0.2821 0.0969 0.015 0.0174 0.4039 0.6725 0.3003 0.1944 0.0573 0.0327
Cell Periphery 0.0208 0.0083 0.0027 0.0016 0.0048 0.0056 0.0067 0.0098 0.0016 0.0072 0.0011 0.0038 0.0143 0.005 0.0041 0.0049 0.0064 0.0069
Cytoplasm 0.0793 0.117 0.0204 0.0241 0.143 0.1506 0.0586 0.152 0.035 0.0625 0.1123 0.161 0.0384 0.0737 0.0195 0.0025 0.0202 0.1047
Cytoplasmic Foci 0.032 0.0025 0.0091 0.0038 0.028 0.011 0.0519 0.0068 0.0103 0.0461 0.0566 0.0136 0.0598 0.0083 0.0272 0.0023 0.0336 0.0187
Eisosomes 0.0022 0.0006 0.0002 0.0001 0.0007 0.0005 0.0007 0.0003 0.0003 0.0015 0.0002 0.0002 0.0013 0.0004 0.0008 0.0003 0.001 0.0005
Endoplasmic Reticulum 0.0041 0.0008 0.0001 0 0.0027 0.0007 0.0015 0.0008 0.0001 0.0008 0.0026 0.0004 0.0037 0.0014 0.0013 0.0001 0.0023 0.0034
Endosome 0.0124 0.0006 0.0005 0.0003 0.0321 0.0028 0.0187 0.0017 0.0016 0.0125 0.0279 0.0039 0.019 0.0007 0.0156 0.0002 0.0106 0.0093
Golgi 0.0115 0.0004 0.0012 0.0006 0.0202 0.0039 0.0106 0.0009 0.0024 0.0072 0.0074 0.0085 0.0108 0.0003 0.0087 0.0006 0.005 0.008
Lipid Particles 0.0112 0.0004 0.0017 0.0001 0.0243 0.0068 0.0188 0.001 0.0019 0.0173 0.0075 0.0053 0.0149 0.0014 0.0072 0.0004 0.008 0.0059
Mitochondria 0.0191 0.0001 0.0002 0 0.0034 0.001 0.028 0.0005 0.0101 0.0035 0.0514 0.0025 0.0201 0.0024 0.0287 0.0007 0.0144 0.0432
None 0.0847 0.0251 0.0007 0.0003 0.0589 0.0448 0.0107 0.0183 0.0008 0.0009 0.0478 0.0063 0.0801 0.0512 0.021 0.0011 0.1019 0.0712
Nuclear Periphery 0.0018 0.0001 0 0 0.0022 0.0001 0.0015 0.0003 0 0.0002 0.0005 0.0001 0.0041 0.0025 0.0028 0.0001 0.0027 0.0046
Nucleolus 0.0024 0.0005 0.0002 0.0005 0.0009 0.0002 0.0015 0.0022 0.0012 0.0031 0.0024 0.0003 0.0057 0.0025 0.0054 0.0008 0.0333 0.0086
Nucleus 0.0047 0.0004 0.0002 0.0003 0.0012 0.0004 0.0016 0.0015 0.0005 0.0007 0.0016 0.0005 0.0325 0.0201 0.0527 0.0007 0.0206 0.0851
Peroxisomes 0.0118 0.0028 0.0128 0.0002 0.0211 0.0044 0.0235 0.0019 0.0148 0.016 0.0048 0.0108 0.0226 0.0012 0.0088 0.0013 0.0201 0.0168
Punctate Nuclear 0.0082 0.0006 0.0006 0.0003 0.018 0.0011 0.0043 0.0007 0.0005 0.0011 0.0023 0.0008 0.0212 0.0058 0.0185 0.0006 0.0345 0.0127
Vacuole 0.0039 0.0009 0.0002 0.0003 0.0012 0.0005 0.0041 0.0074 0.0006 0.004 0.0066 0.0006 0.0061 0.0017 0.0022 0.0008 0.0305 0.0042
Vacuole Periphery 0.0007 0 0 0 0.0005 0.0001 0.0014 0.0005 0.0001 0.0004 0.0006 0.0001 0.0016 0.0003 0.0011 0.0001 0.0081 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.3895 8.4683 10.1088 18.1685 14.6853 31.2849 12.8435 11.1811 15.0829 27.1744
Translational Efficiency 1.6382 1.4054 1.3487 1.1611 2.0305 1.5628 0.9762 1.0591 1.1734 1.162

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘