Standard name
Human Ortholog
Description Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.05 0 0 0 0 0.09 0.12 0.15 0.06 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0.08 0.13 0.17 0.09 0.16 0.24 0.27 0.27 0 0 0 0 0.09 0.08 0 0 0 0 0 0
Nucleus 0.87 0.92 0.76 0.17 0.16 0.23 0.27 0.26 0.29 0.3 0.37 0.37 0.44 0.18 0.1 0.46 0.44 0.39 0.7 0.82 0.52 0.68 0.44 0.29
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.12 0.24 0.83 0.78 0.68 0.64 0.63 0.6 0.57 0.51 0.5 0.51 0.72 0.75 0.49 0.49 0.53 0.26 0.13 0.4 0.21 0.36 0.38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0.05 0.13 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.06 0.08 0.16 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 4 6 2 4 4 3 0 0 0 0 0 0 3 0 0 1 2 6
Bud Neck 0 0 0 0 1 0 1 1 1 1 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 2 0 0 0 1 1 6 6 11 24 17 16 0 2 2 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 2 4 7 11 9 14 29 6 13 4 13 31 53 85 15 11 10 0 1 0 1 4 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 3 5 7 0 0 0 0 0 0
Endosome 0 0 0 1 30 14 27 37 12 11 5 6 4 4 13 0 0 0 0 0 0 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 1 0 0 0 3
Mitochondria 5 4 3 22 29 41 94 57 53 130 110 102 3 4 1 11 17 14 0 0 1 0 5 11
Nucleus 256 217 163 28 56 73 144 165 94 166 150 140 154 76 55 124 88 65 212 203 120 174 177 139
Nuclear Periphery 3 0 5 0 1 4 19 25 14 15 17 14 0 0 1 0 0 0 0 0 1 1 2 6
Nucleolus 53 28 52 140 270 214 346 399 194 310 205 192 179 309 432 132 98 87 78 33 92 54 144 179
Peroxisomes 0 0 0 1 0 0 3 4 4 0 0 0 1 4 5 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 15 15 20 39 15 24 14 10 2 23 72 1 0 0 0 0 0 0 1 4
Vac/Vac Membrane 1 4 6 2 8 9 6 9 10 19 6 8 7 4 6 8 10 10 4 7 13 20 65 115
Unique Cell Count 293 237 215 169 344 313 539 631 323 546 403 382 348 429 574 268 199 165 303 249 234 256 407 478
Labelled Cell Count 325 255 233 202 423 381 685 777 416 717 532 508 382 481 673 294 230 193 303 249 234 256 407 478


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.0 15.7 15.1 14.3 11.8 9.1 8.5 8.1 7.7 6.8 6.3 6.0 18.7 18.1 15.5 37.5 32.1 33.0 21.6 21.0 20.5
Std Deviation (1e-4) 3.4 3.9 5.1 5.1 4.6 3.7 3.1 2.5 3.1 2.3 1.9 2.5 8.8 9.2 7.9 16.1 15.1 15.3 8.0 8.5 7.3
Intensity Change (Log2) -0.08 -0.35 -0.73 -0.83 -0.9 -0.97 -1.15 -1.26 -1.34 0.31 0.26 0.04 1.31 1.09 1.13 0.52 0.48 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.1 3.1 3.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.0 0.7 0.6 1.8 0 0 0 1.1 3.4 4.4 5.1 2.1 2.0 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.5 3.1 3.3 3.6 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.6 3.5 4.8 5.9 3.8 5.6 7.3 7.9 7.8 0 0 0 1.8 3.4 3.3
Nucleus -11.5 -14.0 -11.9 -12.4 -12.9 -10.6 -11.4 -9.1 -9.2 -7.3 -14.4 -18.5 -6.6 -6.6 -7.2
Nuclear Periphery 0 0 0 0.8 1.1 1.2 0.3 1.2 0.9 0 0 0 0 0 0
Nucleolus 11.4 12.6 10.0 9.9 9.9 8.2 8.1 6.4 6.2 6.4 11.5 13.1 5.6 5.3 5.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.1 3.3 2.9 3.7 3.2 3.1 0 0 0 3.5 5.4 0 0 0
Vacuole 0 -0.3 0.1 0 0 0.2 0.5 0 -0.5 0 0 0 0.1 1.2 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.0489 13.5039 12.3687 11.8727 9.4642 12.8995 11.598 16.1711 15.6914 13.8925 12.9838 14.5549 12.3759 13.8354 13.0445 12.64 12.7537 12.4752
Actin 0.0053 0 0.0125 0.0378 0.1086 0 0.0134 0.0067 0.008 0.0256 0.0015 0.0013 0.0005 0 0.0031 0.0013 0.0001 0.0001
Bud 0.0004 0.0001 0.0002 0.0007 0.0032 0.0001 0.0014 0.0002 0.0002 0.0004 0.0002 0.0002 0.0001 0.0001 0.0004 0.0001 0 0.0001
Bud Neck 0.0031 0.0002 0.0014 0.0002 0.0055 0.0004 0.0009 0.0002 0.0003 0.0002 0.0002 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0008
Bud Periphery 0.0012 0.0002 0.0003 0.0009 0.0048 0.0003 0.0016 0.0002 0.0004 0.0003 0.0006 0.0004 0.0003 0.0001 0.0006 0.0003 0.0001 0.0006
Bud Site 0.0025 0.0001 0.0022 0.0009 0.0172 0.0001 0.0041 0.0008 0.0006 0.0011 0.0003 0.0001 0.0003 0.0001 0.0012 0.0002 0 0.0001
Cell Periphery 0.0011 0.0002 0.0002 0.0002 0.0014 0.0003 0.0006 0.0004 0.0002 0.0001 0.0006 0.0005 0.0002 0.0002 0.0003 0.0002 0.0002 0.0006
Cytoplasm 0.0157 0.0002 0.0018 0.0002 0.0141 0.0003 0.0083 0.0038 0.0013 0.0113 0.0002 0.0003 0.0014 0.0002 0.0007 0.0006 0 0.0001
Cytoplasmic Foci 0.017 0.0001 0.0089 0.0024 0.0198 0.0008 0.0388 0.0032 0.0101 0.0141 0.0011 0.0001 0.0031 0 0.0063 0.0005 0.0004 0.0022
Eisosomes 0.0006 0 0.0001 0.0001 0.0003 0 0.0003 0 0.0001 0.0001 0.0002 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0079 0 0.002 0.0002 0.0084 0.0001 0.004 0.0006 0.0008 0.0025 0.0011 0.0004 0.0006 0 0.0003 0.002 0 0.0001
Endosome 0.02 0.0002 0.0053 0.0013 0.0339 0.0002 0.0449 0.0073 0.0079 0.0143 0.0041 0.0013 0.0062 0.0001 0.0066 0.0077 0.0003 0.0003
Golgi 0.0065 0 0.0034 0.0031 0.0084 0 0.0103 0.0018 0.0053 0.0046 0.0078 0.0002 0.0015 0 0.0037 0.0008 0.0003 0.0001
Lipid Particles 0.0117 0.0001 0.0143 0.0012 0.0053 0.0003 0.0244 0.0012 0.0044 0.0068 0.0715 0.0007 0.004 0 0.0067 0.0015 0.0265 0.0031
Mitochondria 0.0046 0.0004 0.0052 0.002 0.0114 0.0003 0.0234 0.002 0.014 0.0045 0.0769 0.0169 0.0128 0.0006 0.0113 0.0019 0.0298 0.0058
None 0.0128 0.0002 0.0012 0.0001 0.0022 0.0002 0.008 0.0002 0.0006 0.0012 0.0004 0.0002 0.0017 0 0.0019 0.0002 0 0.0001
Nuclear Periphery 0.0302 0.005 0.0137 0.0026 0.0644 0.0037 0.0545 0.0148 0.0099 0.0053 0.0265 0.0126 0.0136 0.0051 0.0164 0.0422 0.0134 0.0077
Nucleolus 0.4838 0.4399 0.5677 0.5519 0.2986 0.5554 0.432 0.3716 0.6274 0.4474 0.27 0.4881 0.5304 0.4041 0.6489 0.5228 0.3596 0.5001
Nucleus 0.337 0.5495 0.341 0.3904 0.3627 0.4217 0.2504 0.5699 0.2919 0.4391 0.5153 0.4663 0.4063 0.5809 0.2743 0.4115 0.5522 0.4612
Peroxisomes 0.0025 0 0.0019 0.0021 0.0037 0 0.0034 0.0002 0.0019 0.0166 0.0009 0.0001 0.0002 0 0.0029 0.0001 0.0005 0.0001
Punctate Nuclear 0.0267 0.0017 0.0143 0.0007 0.0098 0.0127 0.0591 0.0016 0.0092 0.0026 0.0006 0.0004 0.0107 0.0001 0.0039 0.0021 0.0008 0.0108
Vacuole 0.0034 0.0011 0.0011 0.0005 0.0081 0.0012 0.0069 0.0095 0.0022 0.0007 0.0025 0.003 0.0025 0.0039 0.003 0.001 0.002 0.0019
Vacuole Periphery 0.006 0.0009 0.0014 0.0005 0.0081 0.0018 0.0092 0.0036 0.0031 0.0011 0.0176 0.0067 0.0037 0.0044 0.007 0.0028 0.0135 0.0039

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 92.4056 112.3102 65.8257 55.4954 77.3144 57.3234 61.7156 41.6539 38.1227 66.5035
Translational Efficiency 2.9632 2.5922 2.7016 2.8253 2.4999 2.8096 2.1013 2.4621 2.184 2.1341

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress
Localization
Cell Percentages nucleus (74%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Nop13

Nop13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available