Standard name
Description Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0.05 0 0 0 0 0.09 0.12 0.15 0.06 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.09 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0.08 0.13 0.17 0.09 0.16 0.24 0.27 0.27 0 0 0 0 0.09 0.08 0 0 0 0 0 0
Nucleus 0.87 0.92 0.76 0.17 0.16 0.23 0.27 0.26 0.29 0.3 0.37 0.37 0.44 0.18 0.1 0.46 0.44 0.39 0.7 0.82 0.52 0.68 0.44 0.29
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.18 0.12 0.24 0.83 0.78 0.68 0.64 0.63 0.6 0.57 0.51 0.5 0.51 0.72 0.75 0.49 0.49 0.53 0.26 0.13 0.4 0.21 0.36 0.38
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0 0.06 0.05 0 0 0 0 0.05 0.13 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0.06 0.08 0.16 0.24
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 4 6 2 4 4 3 0 0 0 0 0 0 3 0 0 1 2 6
Bud Neck 0 0 0 0 1 0 1 1 1 1 0 2 0 0 1 0 0 0 0 0 0 0 0 0
Bud Site 2 0 0 0 1 1 6 6 11 24 17 16 0 2 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4 2 4 7 11 9 14 29 6 13 4 13 31 53 85 15 11 10 0 1 0 1 4 1
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 1 0 0 0 3 5 7 0 0 0 0 0 0
Endosome 0 0 0 1 30 14 27 37 12 11 5 6 4 4 13 0 0 0 0 0 0 0 2 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 0 0 1 0 0 0 3
Mitochondria 5 4 3 22 29 41 94 57 53 130 110 102 3 4 1 11 17 14 0 0 1 0 5 11
Nucleus 256 217 163 28 56 73 144 165 94 166 150 140 154 76 55 124 88 65 212 203 120 174 177 139
Nuclear Periphery 3 0 5 0 1 4 19 25 14 15 17 14 0 0 1 0 0 0 0 0 1 1 2 6
Nucleolus 53 28 52 140 270 214 346 399 194 310 205 192 179 309 432 132 98 87 78 33 92 54 144 179
Peroxisomes 0 0 0 1 0 0 3 4 4 0 0 0 1 4 5 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 0 1 15 15 20 39 15 24 14 10 2 23 72 1 0 0 0 0 0 0 1 4
Vac/Vac Membrane 1 4 6 2 8 9 6 9 10 19 6 8 7 4 6 8 10 10 4 7 13 20 65 115
Unique Cell Count 293 237 215 169 344 313 539 631 323 546 403 382 348 429 574 268 199 165 303 249 234 256 407 478
Labelled Cell Count 325 255 233 202 423 381 685 777 416 717 532 508 382 481 673 294 230 193 303 249 234 256 407 478


Nucleolus, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.0 15.7 15.1 14.3 11.8 9.1 8.5 8.1 7.7 6.8 6.3 6.0 18.7 18.1 15.5 37.5 32.1 33.0 21.6 21.0 20.5
Std Deviation (1e-4) 3.4 3.9 5.1 5.1 4.6 3.7 3.1 2.5 3.1 2.3 1.9 2.5 8.8 9.2 7.9 16.1 15.1 15.3 8.0 8.5 7.3
Intensity Change (Log2) – – – -0.08 -0.35 -0.73 -0.83 -0.9 -0.97 -1.15 -1.26 -1.34 0.31 0.26 0.04 1.31 1.09 1.13 0.52 0.48 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 3.1 3.1 3.0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 1.0 0.7 0.6 1.8 0 0 0 1.1 3.4 4.4 5.1 2.1 2.0 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 4.5 3.1 3.3 3.6 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.6 3.5 4.8 5.9 3.8 5.6 7.3 7.9 7.8 0 0 0 1.8 3.4 3.3
Nucleus -11.5 -14.0 -11.9 -12.4 -12.9 -10.6 -11.4 -9.1 -9.2 -7.3 -14.4 -18.5 -6.6 -6.6 -7.2
Nuclear Periphery 0 0 0 0.8 1.1 1.2 0.3 1.2 0.9 0 0 0 0 0 0
Nucleolus 11.4 12.6 10.0 9.9 9.9 8.2 8.1 6.4 6.2 6.4 11.5 13.1 5.6 5.3 5.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 3.1 3.3 2.9 3.7 3.2 3.1 0 0 0 3.5 5.4 0 0 0
Vacuole 0 -0.3 0.1 0 0 0.2 0.5 0 -0.5 0 0 0 0.1 1.2 1.6