Standard name | |
---|---|
Human Ortholog | |
Description | Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 6 | 2 | 4 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 1 | 2 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 2 | 0 | 0 | 0 | 1 | 1 | 6 | 6 | 11 | 24 | 17 | 16 | 0 | 2 | 2 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 2 | 4 | 7 | 11 | 9 | 14 | 29 | 6 | 13 | 4 | 13 | 31 | 53 | 85 | 15 | 11 | 10 | 0 | 1 | 0 | 1 | 4 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 5 | 7 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1 | 30 | 14 | 27 | 37 | 12 | 11 | 5 | 6 | 4 | 4 | 13 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 3 |
Mitochondria | 5 | 4 | 3 | 22 | 29 | 41 | 94 | 57 | 53 | 130 | 110 | 102 | 3 | 4 | 1 | 11 | 17 | 14 | 0 | 0 | 1 | 0 | 5 | 11 |
Nucleus | 256 | 217 | 163 | 28 | 56 | 73 | 144 | 165 | 94 | 166 | 150 | 140 | 154 | 76 | 55 | 124 | 88 | 65 | 212 | 203 | 120 | 174 | 177 | 139 |
Nuclear Periphery | 3 | 0 | 5 | 0 | 1 | 4 | 19 | 25 | 14 | 15 | 17 | 14 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 6 |
Nucleolus | 53 | 28 | 52 | 140 | 270 | 214 | 346 | 399 | 194 | 310 | 205 | 192 | 179 | 309 | 432 | 132 | 98 | 87 | 78 | 33 | 92 | 54 | 144 | 179 |
Peroxisomes | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 4 | 4 | 0 | 0 | 0 | 1 | 4 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 1 | 15 | 15 | 20 | 39 | 15 | 24 | 14 | 10 | 2 | 23 | 72 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 |
Vac/Vac Membrane | 1 | 4 | 6 | 2 | 8 | 9 | 6 | 9 | 10 | 19 | 6 | 8 | 7 | 4 | 6 | 8 | 10 | 10 | 4 | 7 | 13 | 20 | 65 | 115 |
Unique Cell Count | 293 | 237 | 215 | 169 | 344 | 313 | 539 | 631 | 323 | 546 | 403 | 382 | 348 | 429 | 574 | 268 | 199 | 165 | 303 | 249 | 234 | 256 | 407 | 478 |
Labelled Cell Count | 325 | 255 | 233 | 202 | 423 | 381 | 685 | 777 | 416 | 717 | 532 | 508 | 382 | 481 | 673 | 294 | 230 | 193 | 303 | 249 | 234 | 256 | 407 | 478 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.0 | 15.7 | 15.1 | 14.3 | 11.8 | 9.1 | 8.5 | 8.1 | 7.7 | 6.8 | 6.3 | 6.0 | 18.7 | 18.1 | 15.5 | 37.5 | 32.1 | 33.0 | 21.6 | 21.0 | 20.5 |
Std Deviation (1e-4) | 3.4 | 3.9 | 5.1 | 5.1 | 4.6 | 3.7 | 3.1 | 2.5 | 3.1 | 2.3 | 1.9 | 2.5 | 8.8 | 9.2 | 7.9 | 16.1 | 15.1 | 15.3 | 8.0 | 8.5 | 7.3 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.35 | -0.73 | -0.83 | -0.9 | -0.97 | -1.15 | -1.26 | -1.34 | 0.31 | 0.26 | 0.04 | 1.31 | 1.09 | 1.13 | 0.52 | 0.48 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 3.1 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.0 | 0.7 | 0.6 | 1.8 | 0 | 0 | 0 | 1.1 | 3.4 | 4.4 | 5.1 | 2.1 | 2.0 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 4.5 | 3.1 | 3.3 | 3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 4.6 | 3.5 | 4.8 | 5.9 | 3.8 | 5.6 | 7.3 | 7.9 | 7.8 | 0 | 0 | 0 | 1.8 | 3.4 | 3.3 |
Nucleus | -11.5 | -14.0 | -11.9 | -12.4 | -12.9 | -10.6 | -11.4 | -9.1 | -9.2 | -7.3 | -14.4 | -18.5 | -6.6 | -6.6 | -7.2 |
Nuclear Periphery | 0 | 0 | 0 | 0.8 | 1.1 | 1.2 | 0.3 | 1.2 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 11.4 | 12.6 | 10.0 | 9.9 | 9.9 | 8.2 | 8.1 | 6.4 | 6.2 | 6.4 | 11.5 | 13.1 | 5.6 | 5.3 | 5.7 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 3.1 | 3.3 | 2.9 | 3.7 | 3.2 | 3.1 | 0 | 0 | 0 | 3.5 | 5.4 | 0 | 0 | 0 |
Vacuole | 0 | -0.3 | 0.1 | 0 | 0 | 0.2 | 0.5 | 0 | -0.5 | 0 | 0 | 0 | 0.1 | 1.2 | 1.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 12.0489 | 13.5039 | 12.3687 | 11.8727 | 9.4642 | 12.8995 | 11.598 | 16.1711 | 15.6914 | 13.8925 | 12.9838 | 14.5549 | 12.3759 | 13.8354 | 13.0445 | 12.64 | 12.7537 | 12.4752 |
Actin | 0.0053 | 0 | 0.0125 | 0.0378 | 0.1086 | 0 | 0.0134 | 0.0067 | 0.008 | 0.0256 | 0.0015 | 0.0013 | 0.0005 | 0 | 0.0031 | 0.0013 | 0.0001 | 0.0001 |
Bud | 0.0004 | 0.0001 | 0.0002 | 0.0007 | 0.0032 | 0.0001 | 0.0014 | 0.0002 | 0.0002 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0 | 0.0001 |
Bud Neck | 0.0031 | 0.0002 | 0.0014 | 0.0002 | 0.0055 | 0.0004 | 0.0009 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0008 |
Bud Periphery | 0.0012 | 0.0002 | 0.0003 | 0.0009 | 0.0048 | 0.0003 | 0.0016 | 0.0002 | 0.0004 | 0.0003 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0006 |
Bud Site | 0.0025 | 0.0001 | 0.0022 | 0.0009 | 0.0172 | 0.0001 | 0.0041 | 0.0008 | 0.0006 | 0.0011 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0012 | 0.0002 | 0 | 0.0001 |
Cell Periphery | 0.0011 | 0.0002 | 0.0002 | 0.0002 | 0.0014 | 0.0003 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0002 | 0.0006 |
Cytoplasm | 0.0157 | 0.0002 | 0.0018 | 0.0002 | 0.0141 | 0.0003 | 0.0083 | 0.0038 | 0.0013 | 0.0113 | 0.0002 | 0.0003 | 0.0014 | 0.0002 | 0.0007 | 0.0006 | 0 | 0.0001 |
Cytoplasmic Foci | 0.017 | 0.0001 | 0.0089 | 0.0024 | 0.0198 | 0.0008 | 0.0388 | 0.0032 | 0.0101 | 0.0141 | 0.0011 | 0.0001 | 0.0031 | 0 | 0.0063 | 0.0005 | 0.0004 | 0.0022 |
Eisosomes | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0079 | 0 | 0.002 | 0.0002 | 0.0084 | 0.0001 | 0.004 | 0.0006 | 0.0008 | 0.0025 | 0.0011 | 0.0004 | 0.0006 | 0 | 0.0003 | 0.002 | 0 | 0.0001 |
Endosome | 0.02 | 0.0002 | 0.0053 | 0.0013 | 0.0339 | 0.0002 | 0.0449 | 0.0073 | 0.0079 | 0.0143 | 0.0041 | 0.0013 | 0.0062 | 0.0001 | 0.0066 | 0.0077 | 0.0003 | 0.0003 |
Golgi | 0.0065 | 0 | 0.0034 | 0.0031 | 0.0084 | 0 | 0.0103 | 0.0018 | 0.0053 | 0.0046 | 0.0078 | 0.0002 | 0.0015 | 0 | 0.0037 | 0.0008 | 0.0003 | 0.0001 |
Lipid Particles | 0.0117 | 0.0001 | 0.0143 | 0.0012 | 0.0053 | 0.0003 | 0.0244 | 0.0012 | 0.0044 | 0.0068 | 0.0715 | 0.0007 | 0.004 | 0 | 0.0067 | 0.0015 | 0.0265 | 0.0031 |
Mitochondria | 0.0046 | 0.0004 | 0.0052 | 0.002 | 0.0114 | 0.0003 | 0.0234 | 0.002 | 0.014 | 0.0045 | 0.0769 | 0.0169 | 0.0128 | 0.0006 | 0.0113 | 0.0019 | 0.0298 | 0.0058 |
None | 0.0128 | 0.0002 | 0.0012 | 0.0001 | 0.0022 | 0.0002 | 0.008 | 0.0002 | 0.0006 | 0.0012 | 0.0004 | 0.0002 | 0.0017 | 0 | 0.0019 | 0.0002 | 0 | 0.0001 |
Nuclear Periphery | 0.0302 | 0.005 | 0.0137 | 0.0026 | 0.0644 | 0.0037 | 0.0545 | 0.0148 | 0.0099 | 0.0053 | 0.0265 | 0.0126 | 0.0136 | 0.0051 | 0.0164 | 0.0422 | 0.0134 | 0.0077 |
Nucleolus | 0.4838 | 0.4399 | 0.5677 | 0.5519 | 0.2986 | 0.5554 | 0.432 | 0.3716 | 0.6274 | 0.4474 | 0.27 | 0.4881 | 0.5304 | 0.4041 | 0.6489 | 0.5228 | 0.3596 | 0.5001 |
Nucleus | 0.337 | 0.5495 | 0.341 | 0.3904 | 0.3627 | 0.4217 | 0.2504 | 0.5699 | 0.2919 | 0.4391 | 0.5153 | 0.4663 | 0.4063 | 0.5809 | 0.2743 | 0.4115 | 0.5522 | 0.4612 |
Peroxisomes | 0.0025 | 0 | 0.0019 | 0.0021 | 0.0037 | 0 | 0.0034 | 0.0002 | 0.0019 | 0.0166 | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0029 | 0.0001 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.0267 | 0.0017 | 0.0143 | 0.0007 | 0.0098 | 0.0127 | 0.0591 | 0.0016 | 0.0092 | 0.0026 | 0.0006 | 0.0004 | 0.0107 | 0.0001 | 0.0039 | 0.0021 | 0.0008 | 0.0108 |
Vacuole | 0.0034 | 0.0011 | 0.0011 | 0.0005 | 0.0081 | 0.0012 | 0.0069 | 0.0095 | 0.0022 | 0.0007 | 0.0025 | 0.003 | 0.0025 | 0.0039 | 0.003 | 0.001 | 0.002 | 0.0019 |
Vacuole Periphery | 0.006 | 0.0009 | 0.0014 | 0.0005 | 0.0081 | 0.0018 | 0.0092 | 0.0036 | 0.0031 | 0.0011 | 0.0176 | 0.0067 | 0.0037 | 0.0044 | 0.007 | 0.0028 | 0.0135 | 0.0039 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 92.4056 | 112.3102 | 65.8257 | 55.4954 | 77.3144 | 57.3234 | 61.7156 | 41.6539 | 38.1227 | 66.5035 |
Translational Efficiency | 2.9632 | 2.5922 | 2.7016 | 2.8253 | 2.4999 | 2.8096 | 2.1013 | 2.4621 | 2.184 | 2.1341 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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